FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2117613
Sequences flagged as poor quality0
Sequence length248
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTAGGGCGTCGTGATGATTACACCTGACC44300.2091978090425399No Hit
GTCGTGACTGGGAAAACCCTGGGTACTCGTCGTGATTAACAGGTGTGACC43620.20598664628522773No Hit
GTCGTGACTGGGAAAACCCTGGCCCCTTGTCGTGATATTGAGAAGTGACC43390.20490051770554865No Hit
GTCGTGACTGGGAAAACCCTGGCTCCTGGTCGTGAATAGCGCTATTGACC40270.190166947407293No Hit
GTCGTGACTGGGAAAACCCTGGGATCGTGTCGTGATTGGCACCCCTGACC39870.1882780281382859No Hit
GTCGTGACTGGGAAAACCCTGGAATGAAGTCGTGATGACGCTACATGACC39640.1871918995586068No Hit
GTCGTGACTGGGAAAACCCTGGGATGAGGTCGTGATGACTATCCGTGACC39500.1865307778144543No Hit
GTCGTGACTGGGAAAACCCTGGAGTCACGTCGTGATGAAAACTCTTGACC39310.1856335411616759No Hit
GTCGTGACTGGGAAAACCCTGGGCACGCGTCGTGATGGCATGGGCTGACC39140.18483075047234787No Hit
GTCGTGACTGGGAAAACCCTGGCAGTTGGTCGTGATTTAATGATGTGACC38950.1839335138195695No Hit
GTCGTGACTGGGAAAACCCTGGTTGTACGTCGTGATAATGCCCCGTGACC38940.1838862908378443No Hit
GTCGTGACTGGGAAAACCCTGGACATGGGTCGTGATGAGGACAGTTGACC38660.18256404734953932No Hit
GTCGTGACTGGGAAAACCCTGGACCGCCGTCGTGATTGATAGCCCTGACC38520.18190292560538682No Hit
GTCGTGACTGGGAAAACCCTGGCAAATCGTCGTGATGCCCATCCGTGACC38240.18058068211708184No Hit
GTCGTGACTGGGAAAACCCTGGACTTATGTCGTGATATAAATGGTTGACC38190.18034456720845593No Hit
GTCGTGACTGGGAAAACCCTGGACCGCTGTCGTGATTCGAATGCTTGACC37230.17581116096283883No Hit
GTCGTGACTGGGAAAACCCTGGACGATGGTCGTGATACCCCTGTCTGACC37200.1756694920176633No Hit
GTCGTGACTGGGAAAACCCTGGTCCCCGGTCGTGATTCCGATGAGTGACC37170.17552782307248774No Hit
GTCGTGACTGGGAAAACCCTGGTATAGCGTCGTGATTCCGATCCCTGACC37010.1747722553648849No Hit
GTCGTGACTGGGAAAACCCTGGACCTAGGTCGTGATGAGGATACCTGACC36230.17108886279032098No Hit
GTCGTGACTGGGAAAACCCTGGTCGCCTGTCGTGATGTGTAGAATTGACC35960.16981384228374116No Hit
GTCGTGACTGGGAAAACCCTGGAATCTGGTCGTGATGGCAAGGCATGACC35710.16863326774061171No Hit
GTCGTGACTGGGAAAACCCTGGACTTATGTCGTGATCTGCGCCAGTGACC35670.168444375813711No Hit
GTCGTGACTGGGAAAACCCTGGCTTCTGGTCGTGATTGCCCCCTCTGACC35290.16664990250815423No Hit
GTCGTGACTGGGAAAACCCTGGCGTTGGGTCGTGATTAGGGCACCTGACC35250.1664610105812535No Hit
GTCGTGACTGGGAAAACCCTGGATGACAGTCGTGATAAACATTATTGACC35090.16570544287365066No Hit
GTCGTGACTGGGAAAACCCTGGGACTAGGTCGTGATCTAAAGATATGACC34740.16405263851326943No Hit
GTCGTGACTGGGAAAACCCTGGGTACAAGTCGTGATTATAACCCGTGACC34080.16093592171940765No Hit
GTCGTGACTGGGAAAACCCTGGATGCGTGTCGTGATTTCAGGCGTTGACC33780.1595192322676523No Hit
GTCGTGACTGGGAAAACCCTGGAAAGATGTCGTGATTGGTCGATCTGACC33600.15866921859659908No Hit
GTCGTGACTGGGAAAACCCTGGATCTTCGTCGTGATCGTACAGTCTGACC33480.15810254281589697No Hit
GTCGTGACTGGGAAAACCCTGGATCAGAGTCGTGATGCACAGGGATGACC33280.15715808318139338No Hit
GTCGTGACTGGGAAAACCCTGGTGAGCCGTCGTGATATTGACACCTGACC31370.14813849367188434No Hit
GTCGTGACTGGGAAAACCCTGGATTCCGGTCGTGATCAACGGAACTGACC31170.14719403403738077No Hit
GTCGTGACTGGGAAAACCCTGGAACCCTGTCGTGATACGAAATTGTGACC31090.14681625018357936No Hit
GTCGTGACTGGGAAAACCCTGGGCGATAGTCGTGATATGGGTTTCTGACC30860.14573012160390025No Hit
GTCGTGACTGGGAAAACCCTGGGATTGGGTCGTGATGACATCCCTTGACC30850.14568289862217507No Hit
GTCGTGACTGGGAAAACCCTGGCGTATCGTCGTGATCCGTACGGGTGACC30830.14558845265872472No Hit
GTCGTGACTGGGAAAACCCTGGAACTTAGTCGTGACAGGCTTCCCTGACC30660.14478566196939666No Hit
GTCGTGACTGGGAAAACCCTGGTCTGTCGTCGTGATCCGCGTGATTGACC30380.14346341848109168No Hit
GTCGTGACTGGGAAAACCCTGGGCGGATGTCGTGATTTCGGCTAGTGACC30250.14284951971866436No Hit
GTCGTGACTGGGAAAACCCTGGGCACGTGTCGTGATAAGGGCCCCTGACC30180.1425189588465881No Hit
GTCGTGACTGGGAAAACCCTGGACCGTCGTCGTGATGGGAGCGAGTGACC29990.14162172219380972No Hit
GTCGTGACTGGGAAAACCCTGGGACTTTGTCGTGATGGCGACCCGTGACC29990.14162172219380972No Hit
GTCGTGACTGGGAAAACCCTGGAAACAGGTCGTGATGGTCTCAGCTGACC29970.14152727623035938No Hit
GTCGTGACTGGGAAAACCCTGGCGTGAGGTCGTGATGAGTAGTAGTGACC29930.14133838430345866No Hit
GTCGTGACTGGGAAAACCCTGGGCCAAGGTCGTGATGCACCCACATGACC29500.139307796089276No Hit
GTCGTGACTGGGAAAACCCTGGGGCCTAGTCGTGATTGTCCGCCCTGACC29440.13902445819892492No Hit
GTCGTGACTGGGAAAACCCTGGGCGCTTGTCGTGATTCATATTACTGACC29420.13893001223547458No Hit
GTCGTGACTGGGAAAACCCTGGCTCCATGTCGTGATGTTTCGGTATGACC29230.13803277558269617No Hit
GTCGTGACTGGGAAAACCCTGGGGGTCGGTCGTGATCACAAGGAGTGACC28910.13652164016749047No Hit
GTCGTGACTGGGAAAACCCTGGTGACAAGTCGTGATCAGTGGGTATGACC28850.1362383022771394No Hit
GTCGTGACTGGGAAAACCCTGGAGTTTCGTCGTGATCTGGGCTCTTGACC28770.135860518423338No Hit
GTCGTGACTGGGAAAACCCTGGAGAACCGTCGTGATCAGAGACCTTGACC28750.13576607245988762No Hit
GTCGTGACTGGGAAAACCCTGGTTTTAGGTCGTGATAAGGTCGCATGACC28640.13524661966091064No Hit
GTCGTGACTGGGAAAACCCTGGAAATATCGTCGTGATACTAACAATGACC28490.13453827493503298No Hit
GTCGTGACTGGGAAAACCCTGGACCAAGGTCGTGATAGGGCAACTTGACC28090.13264935566602584No Hit
GTCGTGACTGGGAAAACCCTGGTGCGTTGTCGTGATCGGATCTCTTGACC28070.1325549097025755No Hit
GTCGTGACTGGGAAAACCCTGGCCGATAGTCGTGATTGGGAATTATGACC28060.13250768672085034No Hit
GTCGTGACTGGGAAAACCCTGGAACGTTGTCGTGATGTTGGCGCTTGACC28020.1323187947939496No Hit
GTCGTGACTGGGAAAACCCTGGGTTTAAGTCGTGATTGGAAGTAGTGACC27920.13184656497669783No Hit
GTCGTGACTGGGAAAACCCTGGAACATAGTCGTGATCTAATGTTGTGACC27200.128446510292485No Hit
GTCGTGACTGGGAAAACCCTGGTGACAGGTCGTGATGTCAGGATCTGACC27110.1280215034569584No Hit
GTCGTGACTGGGAAAACCCTGGGCGTCCGTCGTGATGCCGAGTATTGACC26940.12721871276763036No Hit
GTCGTGACTGGGAAAACCCTGGACCAGGGTCGTGATCTCTGGGGTTGACC26930.12717148978590517No Hit
GTCGTGACTGGGAAAACCCTGGTTGCGTGTCGTGATATGTAGGGATGACC26730.1262270301514016No Hit
GTCGTGACTGGGAAAACCCTGGCCCCCTGTCGTGATCGCGCGAAGTGACC26510.12518812455344766No Hit
GTCGTGACTGGGAAAACCCTGGACGGAGGTCGTGATTGTTGGAGTTGACC26310.1242436649189441No Hit
GTCGTGACTGGGAAAACCCTGGCTGGTAGTCGTGATGAACAGAAGTGACC26210.12377143510169232No Hit
GTCGTGACTGGGAAAACCCTGGTCCCCTGTCTGATACTTTGACAATGACC26170.12358254317479161No Hit
GTCGTGACTGGGAAAACCCTGGGTGTCCGTCGTGATAGCAAGACATGACC26080.12315753633926502No Hit
GTCGTGACTGGGAAAACCCTGGCTGGATGTCGTGATGTTAACTCTTGACC25920.12240196863166217No Hit
GTCGTGACTGGGAAAACCCTGGCTATGCGTCGTGATATGTACAACTGACC25740.12155195496060896No Hit
GTCGTGACTGGGAAAACCCTGGTACTGTGTCGTGATGTAGTAAACTGACC25740.12155195496060896No Hit
GTCGTGACTGGGAAAACCCTGGATGGATGTCGTGATTAAAAGTAGTGACC25670.1212213940885327No Hit
GTCGTGACTGGGAAAACCCTGGCACATGGTCGTGATCGAGCAACATGACC25520.12051304936265503No Hit
GTCGTGACTGGGAAAACCCTGGTTCCGGGTCGTGATTCATGTTACTGACC25520.12051304936265503No Hit
GTCGTGACTGGGAAAACCCTGGAAAACAGTCGTGATACGGGGAACTGACC25440.1201352655088536No Hit
GTCGTGACTGGGAAAACCCTGGGGACGAGTCGTGATGTTTACCCCTGACC25370.11980470463677736No Hit
GTCGTGACTGGGAAAACCCTGGAAGGGGGTCGTGATAATCTGACATGACC24820.11720744064189254No Hit
GTCGTGACTGGGAAAACCCTGGAGATCGGTCGTGATTCAGGGTATTGACC24770.11697132573326666No Hit
GTCGTGACTGGGAAAACCCTGGCTAGTCGTCGTGATCGCGCGGAGTGACC24740.11682965678809112No Hit
GTCGTGACTGGGAAAACCCTGGTCGATCGTCGTGATCACTGGAAATGACC24700.11664076486119042No Hit
GTCGTGACTGGGAAAACCCTGGCCGATCGTCGTGATCAGCCGGGTTGACC24530.11583797417186238No Hit
GTCGTGACTGGGAAAACCCTGGGTGGCAGTCGTGATCCTAATGATTGACC24450.11546019031806094No Hit
GTCGTGACTGGGAAAACCCTGGCCATCCGTCGTGATAATTCTGGTTGACC24200.11427961577493148No Hit
GTCGTGACTGGGAAAACCCTGGAATGCGGTCGTGATCCCCCGTCTTGACC24190.11423239279320632No Hit
GTCGTGACTGGGAAAACCCTGGTCTGGTTGTCGTGATGCCAGGGGTGACC24070.11366571701250418No Hit
GTCGTGACTGGGAAAACCCTGGACATCTGTCGTGATAAAATGGGTTGACC23990.11328793315870274No Hit
GTCGTGACTGGGAAAACCCTGGTCTGCAGTCGTGATCAGAACAATTGACC23940.11305181825007685No Hit
GTCGTGACTGGGAAAACCCTGGTCCGGTGTCGTGATACCGAAGATTGACC23890.11281570334145097No Hit
GTCGTGACTGGGAAAACCCTGGTGCGCAGTCGTGATAAAATTTTGTGACC23840.11257958843282508No Hit
GTCGTGACTGGGAAAACCCTGGGTCACAGTCGTGATCTTTATGAATGACC23840.11257958843282508No Hit
GTCGTGACTGGGAAAACCCTGGTGTTGCGTCGTGATCATAATGTGTGACC23760.11220180457902364No Hit
GTCGTGACTGGGAAAACCCTGGCAACAAGTCGTGATCAGTATTCTTGACC23760.11220180457902364No Hit
GTCGTGACTGGGAAAACCCTGGGAGTTAGTCGTGATGGTAGAGGGTGACC23720.11201291265212293No Hit
GTCGTGACTGGGAAAACCCTGGAGTATGGTCGTGATCCGAAGTGTTGACC23680.11182402072522223No Hit
GTCGTGACTGGGAAAACCCTGGGTTAGAGTCGTGATCCCCGGCGTTGACC23670.11177679774349704No Hit
GTCGTGACTGGGAAAACCCTGGCTGCAAGTCGTGATCAGAAGGTGTGACC23640.11163512879832149No Hit
GTCGTGACTGGGAAAACCCTGGTTCGTGGTCGTGATCGTACGGATTGACC23590.11139901388969563No Hit
GTCGTGACTGGGAAAACCCTGGGAATGTGTCGTGATCCTCCGATATGACC23470.11083233810899348No Hit
GTCGTGACTGGGAAAACCCTGGCCTCTTGTCGTGATCGTGAGGGATGACC23470.11083233810899348No Hit
GTCGTGACTGGGAAAACCCTGGTCTTATGTCGTGATAACAGAAGCTGACC23430.11064344618209275No Hit
GTCGTGACTGGGAAAACCCTGGCAAATGGTCGTGATGCTTGTATCTGACC23380.11040733127346689No Hit
GTCGTGACTGGGAAAACCCTGGTTTACCGTCGTGATATAAGCTCCTGACC23360.1103128853100165No Hit
GTCGTGACTGGGAAAACCCTGGTATCCAGTCTGATTTGGATGATCTGACC23250.10979343251103955No Hit
GTCGTGACTGGGAAAACCCTGGTCACACGTCGTGATCGTTCGTAGTGACC23220.10965176356586401No Hit
GTCGTGACTGGGAAAACCCTGGGTGCCCGTCGTGATCATGGATAATGACC23140.1092739797120626No Hit
GTCGTGACTGGGAAAACCCTGGTGCACGGTCGTGATAGATTCACTTGACC23000.10861285796791009No Hit
GTCGTGACTGGGAAAACCCTGGCCCCTTGTCGTGATAGGTTGAGTTGACC22960.10842396604100939No Hit
GTCGTGACTGGGAAAACCCTGGTCGCGTGTCGTGATTCCTATTACTGACC22880.10804618218720795No Hit
GTCGTGACTGGGAAAACCCTGGCCTATAGTCGTGATTCATATGACTGACC22870.10799895920548279No Hit
GTCGTGACTGGGAAAACCCTGGGCCGAAGTCGTGATTGCTGGGGGTGACC22800.10766839833340654No Hit
GTCGTGACTGGGAAAACCCTGGTACTTAGTCGTGATTCAGGTTCCTGACC22780.10757395236995616No Hit
GTCGTGACTGGGAAAACCCTGGACTCAAGTCGTGATAACCGGTCATGACC22700.10719616851615475No Hit
GTCGTGACTGGGAAAACCCTGGAAGAACGTCGTGATGAAATAGGATGACC22570.10658226975372743No Hit
GTCGTGACTGGGAAAACCCTGGATTACTGTCGTGATCTTACTTTATGACC22420.10587392502784976No Hit
GTCGTGACTGGGAAAACCCTGGCCCGAGGTCGTGATTAGAATCCTTGACC22380.10568503310094904No Hit
GTCGTGACTGGGAAAACCCTGGGCGAGAGTCGTGATGTATCCACTTGACC22370.10563781011922385No Hit
GTCGTGACTGGGAAAACCCTGGTGACACGTCGTGATCTTCTAGATTGACC22300.10530724924714761No Hit
GTCGTGACTGGGAAAACCCTGGACATTAGTCGTGATTAGACCGTGTGACC22250.10507113433852172No Hit
GTCGTGACTGGGAAAACCCTGGCTGCTGGTCGTGATCACGGTGAGTGACC22240.10502391135679655No Hit
GTCGTGACTGGGAAAACCCTGGTGAGGAGTCGTGATATTTAAATGTGACC22130.10450445855781959No Hit
GTCGTGACTGGGAAAACCCTGGTTGCCCGTCGTGATCTTACCTCCTGACC22030.1040322287405678No Hit
GTCGTGACTGGGAAAACCCTGGGCATAAGTCGTGATCCACTACAGTGACC22000.10389055979539226No Hit
GTCGTGACTGGGAAAACCCTGGTAGTCAGGTCGTGATTCAGATGCTGACC21980.10379611383194191No Hit
GTCGTGACTGGGAAAACCCTGGTCCGACGTCGTGATCATAGAGATTGACC21910.10346555295986566No Hit
GTCGTGACTGGGAAAACCCTGGGCTCCCGTCGTGATATGAATTACTGACC21880.10332388401469011No Hit
GTCGTGACTGGGAAAACCCTGGATATCCGTCGTGATCCATATGCATGACC21750.10270998525226281No Hit
GTCGTGACTGGGAAAACCCTGGTTAATTGTCGTGATCTTGACTCATGACC21750.10270998525226281No Hit
GTCGTGACTGGGAAAACCCTGGTCGCGAGTCGTGATACTCACCACTGACC21740.10266276227053762No Hit
GTCGTGACTGGGAAAACCCTGGTGAAATGTCGTGATCAGTGCAAATGACC21720.10256831630708726No Hit
GTCGTGACTGGGAAAACCCTGGAGCGCTGTCGTGATAGTCATAGCTGACC21660.1022849784167362No Hit
GTCGTGACTGGGAAAACCCTGGGGGGATGTCGTGATATGTAAATCTGACC21560.10181274859948442No Hit
GTCGTGACTGGGAAAACCCTGGGACTCTGTCGTGATTAATACAGTTGACC21560.10181274859948442No Hit
GTCGTGACTGGGAAAACCCTGGCACTCCGTCGTGATTGCAAGATGTGACC21390.1010099579101564No Hit
GTCGTGACTGGGAAAACCCTGGTTGGTTGTCGTGATGCGGCCATTTGACC21360.10086828896498086No Hit
GTCGTGACTGGGAAAACCCTGGTAATCGGTCGTGATTGGCGGTAGTGACC21360.10086828896498086No Hit
GTCGTGACTGGGAAAACCCTGGGACTCCGTCGTGATAGCTTCTCCTGACC21300.10058495107462978No Hit
GTCGTGACTGGGAAAACCCTGGGATGAAGTCGTGATTACGATTAATGACC21270.10044328212945426No Hit
GTCGTGACTGGGAAAACCCTGGCCTCCGGTCGTGATGCCTATAAGTGACC21240.10030161318427872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG1954550.0240.989655
GTGACTG1959200.0240.661974
TCGTGAC1943750.0240.277832
ACTGGGA1973300.0239.948847
TGGGAAA1925450.0239.916219
GACTGGG1994950.0238.760396
CTGGGAA2037600.0231.46348
CGTGACT2022200.0227.377443
GACGGGG40950.0225.891256
TCGAGAC3500.0225.159682
CGTGACG23500.0221.222493
ACGGGGA40950.0211.057927
GTGACGG38200.0210.537224
CTGCGAA1650.0205.40018
TGACGGG39500.0203.885885
TCGGGAC22650.0199.122362
GTGGTGA79800.0193.014531
CGTGACA29200.0190.90393
GGTGACT66750.0180.820623
CGGGGAA47250.0179.573728
Produced by FastQC (version 0.11.5)