FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914087
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTATGTGGTCGTGATTCTGATACCTTAGG17180.18794709912732596No Hit
GTCGTGACTGGGAAAACCCTGGATTAGCGTCGTGATGAATATAGTTTAGG16230.17755421529898138No Hit
GTCGTGACTGGGAAAACCCTGGAATCGTGTCGTGATTTAACTGGATTAGG16090.1760226324190148No Hit
GTCGTGACTGGGAAAACCCTGGACCCCTGTCGTGATAAGACGCCTTTAGG15860.17350646054478402No Hit
GTCGTGACTGGGAAAACCCTGGCATTCAGTCGTGATGCGTAGGGATTAGG15460.16913050945916525No Hit
GTCGTGACTGGGAAAACCCTGGCGGAGTGTCGTGATAACTGAGTGTTAGG14260.15600265620230896No Hit
GTCGTGACTGGGAAAACCCTGGGACATGGTCGTGATTCTTAGTATTTAGG14170.15501806720804476No Hit
GTCGTGACTGGGAAAACCCTGGCTACCGGTCGTGATCGGGCGGAGTTAGG14080.15403347821378052No Hit
GTCGTGACTGGGAAAACCCTGGTGAGCAGTCGTGATACTGTGGTGTTAGG13790.15086091367670693No Hit
GTCGTGACTGGGAAAACCCTGGTTCCGCGTCGTGATACCACGGGTTTAGG13730.1502045210138641No Hit
GTCGTGACTGGGAAAACCCTGGCCATTTGTCGTGATTGCAGTCTTTTAGG13560.14834474180247612No Hit
GTCGTGACTGGGAAAACCCTGGGAAGTCGTCGTGATACAAGCGGATTAGG12910.14123382128834566No Hit
GTCGTGACTGGGAAAACCCTGGCCTTACGTCGTGATCCTTAGCCCTTAGG12660.1384988518598339No Hit
GTCGTGACTGGGAAAACCCTGGTACAAGGTCGTGATATCCAGGTGTTAGG12570.1375142628655697No Hit
GTCGTGACTGGGAAAACCCTGGCACTTTGTCGTGATGCGGGGTATTTAGG12570.1375142628655697No Hit
GTCGTGACTGGGAAAACCCTGGAAATTGGTCGTGATACCTGGGTATTAGG12510.13685787020272686No Hit
GTCGTGACTGGGAAAACCCTGGCATTGTGTCGTGATAGGACTAAGTTAGG12460.13631087631702454No Hit
GTCGTGACTGGGAAAACCCTGGACGCCAGTCGTGATCGGGATTATTTAGG12060.13193492523140576No Hit
GTCGTGACTGGGAAAACCCTGGAAGTTGGTCGTGATTTGGATTTCTTAGG12050.1318255264542653No Hit
GTCGTGACTGGGAAAACCCTGGTCATTGGTCGTGATAAATAAATGTTAGG12020.13149733012284387No Hit
GTCGTGACTGGGAAAACCCTGGGGCAAGGTCGTGATGTTAATGGATTAGG11890.1300751460200178No Hit
GTCGTGACTGGGAAAACCCTGGCCATACGTCGTGATTCAAACGAGTTAGG11870.12985634846573685No Hit
GTCGTGACTGGGAAAACCCTGGTGTTCCGTCGTGATTTGAGGTATTTAGG11760.1286529619171917No Hit
GTCGTGACTGGGAAAACCCTGGTCCTACGTCGTGATGTGCACGCTTTAGG11550.12635558759724183No Hit
GTCGTGACTGGGAAAACCCTGGGTCCAAGTCGTGATAAAGGGGAGTTAGG11400.1247146059401348No Hit
GTCGTGACTGGGAAAACCCTGGTACTTAGTCGTGATGACGCATCTTTAGG11270.12329242183730871No Hit
GTCGTGACTGGGAAAACCCTGGTGATGAGGTCGTGATCTGTCCTGTTAGG11220.12274542795160635No Hit
GTCGTGACTGGGAAAACCCTGGTGTGACGTCGTGATCGAGGCTGATTAGG11150.12197963651162307No Hit
GTCGTGACTGGGAAAACCCTGGCATCGTGTCGTGATTTTAACATTTTAGG10940.11968226219167323No Hit
GTCGTGACTGGGAAAACCCTGGACCTACGTCGTGATGAAAGTGGGTTAGG10610.11607210254603775No Hit
GTCGTGACTGGGAAAACCCTGGGAACGCGTCGTGATGAAGTTGGATTAGG10310.11279013923182367No Hit
GTCGTGACTGGGAAAACCCTGGGGTGTAGTCGTGATCTTTCCAAATTAGG10180.11136795512899757No Hit
GTCGTGACTGGGAAAACCCTGGGGGGCCGTCGTGATCAAAAATTGTTAGG10160.11114915757471663No Hit
GTCGTGACTGGGAAAACCCTGGCGAACAGTCGTGATCAGAATGGTTTAGG10160.11114915757471663No Hit
GTCGTGACTGGGAAAACCCTGGACGATGGTCGTGATGATGGGACGTTAGG10150.11103975879757616No Hit
GTCGTGACTGGGAAAACCCTGGATACGAGTCGTGATCATGTTCCCTTAGG10090.11038336613473335No Hit
GTCGTGACTGGGAAAACCCTGGTGGGCGGTCGTGATACGAGAAAATTAGG9520.1041476358377266No Hit
GTCGTGACTGGGAAAACCCTGGTTTTCTGTCGTGATGAGTACACCTTAGG9440.10327244562060285No Hit
GTCGTGACTGGGAAAACCCTGGATGAATGTCGTGATGCATGTTATTTAGG9430.10316304684346238No Hit
GTCGTGACTGGGAAAACCCTGGTGGTCAGTCGTGATAAGGCCATCTTAGG9430.10316304684346238No Hit
GTCGTGACTGGGAAAACCCTGGCACGTAGTCGTGATAAGCAGTACTTAGG9300.10174086274063628No Hit
GTCGTGACTGGGAAAACCCTGGTTCGTAGTCGTGATAACAAGAGCTTAGG9280.10152206518635534No Hit
GTCGTGACTGGGAAAACCCTGGACCGTAGTCGTGATATTACTTCATTAGG9270.10141266640921488No Hit
GTCGTGACTGGGAAAACCCTGGTTGCTGGTCGTGATCAAGATTATTTAGG9180.10042807741495066No Hit
GTCGTGACTGGGAAAACCCTGGACAAGCGTCGTGATCTGAAGATTTTAGG9170.10031867863781019No Hit
GTCGTGACTGGGAAAACCCTGGACCTATGTCGTGATAACGCAGACTTAGG9160.10020927986066973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC816650.0241.356022
ACTGGGA839300.0241.149557
TGGGAAA818450.0240.132269
TGACTGG844100.0238.190225
GTGACTG845850.0238.115484
CGTGACT818050.0237.446213
GACTGGG857350.0237.255256
CTGGGAA859900.0234.696768
ACGGGGA19300.0228.316577
GACGGGG21700.0218.163316
GGTGACT33850.0218.04773
GTGGTGA43600.0213.68421
GTGACAG12350.0209.963854
TGGTGAC42050.0208.487212
CGTGACG12000.0206.035393
CGTGACA12450.0199.561783
GTGACGG20250.0198.660164
TGACGGG21000.0192.047445
GGGACTG16200.0182.504034
TGACAGG14050.0181.019995
Produced by FastQC (version 0.11.5)