FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324847
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGATGCCGTCGTGATGCAATAGAGACTTG94670.40720959271728413No Hit
GTCGTGACTGGGAAAACCCTGGTAATTGGTCGTGATACCGTCTTAACTTG51710.22242323903465477No Hit
GTCGTGACTGGGAAAACCCTGGCTACCTGTCGTGATCAATTGGCTACTTG37020.15923628522651168No Hit
GTCGTGACTGGGAAAACCCTGGGCTCTTGTCGTGATCTGATTTATACTTG35680.15347246506974438No Hit
GTCGTGACTGGGAAAACCCTGGACGGTTGTCGTGATCAAAAGTGCACTTG35480.15261219340455523No Hit
GTCGTGACTGGGAAAACCCTGGTTCTGTGTCGTGATGACGACACTACTTG33380.14357934092006913No Hit
GTCGTGACTGGGAAAACCCTGGTATGCGGTCGTGATCAAAGTCTTACTTG33300.14323523225399348No Hit
GTCGTGACTGGGAAAACCCTGGCCGAGGGTCGTGATTATAACAAGACTTG33220.14289112358791783No Hit
GTCGTGACTGGGAAAACCCTGGAGGCCGGTCGTGATAACCAGAGTACTTG33050.14215989267250703No Hit
GTCGTGACTGGGAAAACCCTGGTTCGGTGGATAGTAGCTACCTAAACTTG32300.13893387392804774No Hit
GTCGTGACTGGGAAAACCCTGGCCTCGCGTCGTGATGTACAAATGACTTG31300.134632515602102No Hit
GTCGTGACTGGGAAAACCCTGGTCGATAGTCGTGATATTGTGATCACTTG30320.13041718444267514No Hit
GTCGTGACTGGGAAAACCCTGGCAACTCGTCGTGATCTTCAGTTGACTTG29950.12882568186207524No Hit
GTCGTGACTGGGAAAACCCTGGAATCACGTCGTGATGAGGGGTCGACTTG29470.12676102986562127No Hit
GTCGTGACTGGGAAAACCCTGGGTTAGTGTCGTGATTGACAGGGGACTTG29360.12628788044976724No Hit
GTCGTGACTGGGAAAACCCTGGGCCTCAGTCGTGATGATATTACTACTTG28940.12448130995287002No Hit
GTCGTGACTGGGAAAACCCTGGCGAATAGTCGTGATAACAAGATTACTTG28580.12293282095552954No Hit
GTCGTGACTGGGAAAACCCTGGAAAATTGTCGTGATGCGCGTGCCACTTG28570.1228898073722701No Hit
GTCGTGACTGGGAAAACCCTGGACCCTGGTCGTGATACCTTCAGGACTTG28540.12276076662249172No Hit
GTCGTGACTGGGAAAACCCTGGGATTGAGTCGTGATCAAAGCGTTACTTG28140.12104022329211342No Hit
GTCGTGACTGGGAAAACCCTGGGGCAGTGTCGTGATCAATAAAGAACTTG28090.12082515537581613No Hit
GTCGTGACTGGGAAAACCCTGGGTCCTAGTCGTGATCCTGACTGAACTTG27670.11901858487891892No Hit
GTCGTGACTGGGAAAACCCTGGAGAGAGGTCGTGATGTTAAGTTAACTTG27510.11833036754676758No Hit
GTCGTGACTGGGAAAACCCTGGGATTATGTCGTGATGGGCAGGGGACTTG27370.11772817738113518No Hit
GTCGTGACTGGGAAAACCCTGGCCTTCAGTCGTGATCTGGAGATGACTTG27330.11755612304809736No Hit
GTCGTGACTGGGAAAACCCTGGCTACCCGTCGTGATCAACTGGATACTTG26910.11574955255120015No Hit
GTCGTGACTGGGAAAACCCTGGCTAGCCGTCGTGATTACAGGATGACTTG26680.11476024013623261No Hit
GTCGTGACTGGGAAAACCCTGGCGGATGGTCGTGATCTACACACAACTTG26650.11463119938645425No Hit
GTCGTGACTGGGAAAACCCTGGTCCAGCGTCGTGATCGAAAGAAGACTTG26080.11217942514066517No Hit
GTCGTGACTGGGAAAACCCTGGATTCGAGTCGTGATTAAAACGACACTTG25890.11136216705873547No Hit
GTCGTGACTGGGAAAACCCTGGATTATTGGTCGTGATTGTCTGGAACTTG25830.11110408555917872No Hit
GTCGTGACTGGGAAAACCCTGGTCATCAGTCGTGATTACATATAGACTTG25750.11075997689310307No Hit
GTCGTGACTGGGAAAACCCTGGAAATCGGTCGTGATCGCACCGGAACTTG25640.11028682747724905No Hit
GTCGTGACTGGGAAAACCCTGGCTGGTCGTCGTGATATTAGGTGTACTTG25540.10985669164465446No Hit
GTCGTGACTGGGAAAACCCTGGGACAGCGTCGTGATAAGAGGATCACTTG25390.1092114878957626No Hit
GTCGTGACTGGGAAAACCCTGGAAAATTGTCGTGATACGAGTGGCACTTG24830.10680272723323299No Hit
GTCGTGACTGGGAAAACCCTGGTGCGAGGTCGTGATACCCTGGAGACTTG24640.10598546915130329No Hit
GTCGTGACTGGGAAAACCCTGGGCACTAGTCGTGATCTGAAGGGGACTTG24580.10572738765174655No Hit
GTCGTGACTGGGAAAACCCTGGCCCCTAGTCGTGATCTATTTGGTACTTG24540.10555533331870871No Hit
GTCGTGACTGGGAAAACCCTGGACGTACGTCGTGATGGATCGTTGACTTG24360.10478108882003848No Hit
GTCGTGACTGGGAAAACCCTGGCAATAAGTCGTGATACTAGGGGAACTTG23700.10194219232491429No Hit
GTCGTGACTGGGAAAACCCTGGATACAAGTCGTGATATGAGATGGACTTG23650.101727124408617No Hit
GTCGTGACTGGGAAAACCCTGAACAAGTCGTGATGTGGTGCGGAGACTTG23610.10155507007557918No Hit
GTCGTGACTGGGAAAACCCTGGAGTTTAGTCGTGATTACAAGACTACTTG23520.10116794782624404No Hit
GTCGTGACTGGGAAAACCCTGGAGCGCAGTCGTGATGTCGTCGCAACTTG23480.10099589349320622No Hit
GTCGTGACTGGGAAAACCCTGGAATGCAGTCGTGATGAGTATCCCACTTG23470.10095287990994675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC2083250.0241.787262
TGACTGG2133500.0241.204975
GTGACTG2139200.0240.720234
GACTGGG2169400.0240.643116
CGTGACT2055700.0240.637793
ACTGGGA2160650.0240.471867
TGGGAAA2131500.0236.804269
GACGGGG57000.0232.809276
CTGGGAA2247200.0230.209088
CGTGACA25300.0224.15823
TGACGGG51100.0222.992035
GCGTGAC17650.0220.493352
CGTGACG32400.0218.796393
GTGGTGA125750.0206.891051
TCGGGAC28350.0203.558412
GGTGACT101450.0201.508013
GTGACGG57200.0200.114234
TGGTGAC125750.0195.03972
TGAGTGG16450.0194.338425
GGACGGG11650.0191.25495
Produced by FastQC (version 0.11.5)