FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345206
Sequences flagged as poor quality0
Sequence length248
%GC65

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGACCCTGCCGTCGTGATGCCGCGAGATCAC34320.2551282108465172No Hit
GTCGTGACTGGGAAAACCCTGGATCACTGTCGTGATTTTTGCAGAATCAC31060.230894004338369No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTCGTGATAAAAAAAGATCAC14240.10585739284540807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC1272850.0242.086362
CGTGACT1256350.0242.02363
GTGACTG1275100.0241.49394
TGACTGG1274800.0241.36435
ACTGGGA1272500.0241.321677
GACTGGG1279200.0241.021946
TGGGAAA1255950.0240.599559
CTGGGAA1280050.0239.180738
GTGACAG16000.0233.187684
CGTGACA15950.0224.913793
GGTGACT18200.0222.412963
TGACAGG16800.0221.30485
GTGGTGA25100.0220.90051
GTGACGG10250.0217.454714
AGGGAAA17700.0211.994229
TGGTGAC24150.0211.409712
TCGTGGC6800.0210.442342
CGTGACG7050.0209.727663
TCGGGAC4850.0205.036942
ACAGGGA18300.0204.397357
Produced by FastQC (version 0.11.5)