FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320076
Sequences flagged as poor quality0
Sequence length248
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTTCATTGTCGTGATCTCAGGACTTGACC33301.0403779102463164No Hit
GTCGTGACTGGGAAAACCCTGGCCAGTGGTCGTGATTCGTTGTGCTGACC17000.5311238580837051No Hit
GTCGTGACTGGGAAAACCCTGGGGTACGGTCTGATTATAGACCGTTGACC6660.20807558204926332No Hit
GTCGTGACTGGGAAAACCCTGGATGAAGGTCGTGATACAGGCGCATGACC6100.1905797373123883No Hit
GTCGTGACTGGGAAAACCCTGGAAGTTTGTCGTGATGCCTGGGTATGACC5760.1799572601507142No Hit
GTCGTGACTGGGAAAACCCTGGGTCTAAGTCGTGATAGAAAGAGCTGACC5490.17152176358114948No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGACC5380.16808507979354903No Hit
GTCGTGACTGGGAAAACCCTGGACGAACGTCGTGATACCATCTAGTGACC5280.16496082180482136No Hit
GTCGTGACTGGGAAAACCCTGGCGTCGTGTCGTGATGCTGTCCCCTGACC5260.16433597020707583No Hit
GTCGTGACTGGGAAAACCCTGGCGAGTCGTCGTGATAATGATTCCTGACC5220.16308626701158474No Hit
GTCGTGACTGGGAAAACCCTGGCATATTGTCGTGATGTCTTCGCTTGACC4900.1530886414476562No Hit
GTCGTGACTGGGAAAACCCTGGAGTCCCGTCGTGATCACATAGGCTGACC4870.15215136405103788No Hit
GTCGTGACTGGGAAAACCCTGGCAGCTGGTCGTGATTCCCTCTGCTGACC4830.15090166085554682No Hit
GTCGTGACTGGGAAAACCCTGGGTTACAGTCGTGATTCGGGTGACTGACC4710.1471525512690736No Hit
GTCGTGACTGGGAAAACCCTGGAACCTAGTCGTGATGTGAATTCCTGACC4690.14652769967132806No Hit
GTCGTGACTGGGAAAACCCTGGCAGTTGTCGTGATTTGCGTCTGCTGACC4680.14621527387245528No Hit
GTCGTGACTGGGAAAACCCTGGCTAGGTGTCGTGTCCCAGCCCTGTGACC4640.14496557067696422No Hit
GTCGTGACTGGGAAAACCCTGGGACAATGTCGTGATACTAGCGGGTGACC4520.141216461090491No Hit
GTCGTGACTGGGAAAACCCTGGGGGGTTGTCGTGAGAAACACCCTTGACC4430.1384046289006361No Hit
GTCGTGACTGGGAAAACCCTGGTCCTCGGTCGTGATGGAAAGTGGTGACC4390.13715492570514504No Hit
GTCGTGACTGGGAAAACCCTGGTGCGTAGTCGTGATTGGGTTCTGTGACC4380.13684249990627226No Hit
GTCGTGACTGGGAAAACCCTGGACTCCCGTCGTGATACCTATCCGTGACC4360.13621764830852673No Hit
GTCGTGACTGGGAAAACCCTGGAATCTAGTCGTGATTTATACGAGTGACC4310.1346555193141629No Hit
GTCGTGACTGGGAAAACCCTGGGCCGCAGTCGTGATACCCGTGGCTGACC4310.1346555193141629No Hit
GTCGTGACTGGGAAAACCCTGGTTTGGTGTCGTGATAATTGCAAATGACC4290.13403066771641736No Hit
GTCGTGACTGGGAAAACCCTGGCTGAGCGTCGTGATGAAGGCGAGTGACC4280.13371824191754458No Hit
GTCGTGACTGGGAAAACCCTGGTTTTTTGTCGTGATTTGATCAGGTGACC4250.13278096452092628No Hit
GTCGTGACTGGGAAAACCCTGGGTTGTCGTCGTGATTGAAACCCTTGACC4210.13153126132543522No Hit
GTCGTGACTGGGAAAACCCTGGCCTAACAGTCGTGATGCCAAGGGTGACC4110.12840700333670754No Hit
GTCGTGACTGGGAAAACCCTGGGGCCCCGTCGTGATATTTGAATATGACC4100.12809457753783476No Hit
GTCGTGACTGGGAAAACCCTGGCTCGAAGTCGTGATTCAATGGCGTGACC4100.12809457753783476No Hit
GTCGTGACTGGGAAAACCCTGGTCGGGAGTCGTGATAGTCGCTGATGACC4080.12746972594008923No Hit
GTCGTGACTGGGAAAACCCTGGGACCTCGTCGTGATTGCACGGGCTGACC4070.12715730014121646No Hit
GTCGTGACTGGGAAAACCCTGGACTGCATGTCGTGATGGAAACATTGACC3980.12434546795136155No Hit
GTCGTGACTGGGAAAACCCTGGAATTAAGTCGGATGAAGGCGATATGACC3920.12247091315812494No Hit
GTCGTGACTGGGAAAACCCTGGCCCGCAGTCGTGATTGTAGTTGCTGACC3860.12059635836488833No Hit
GTCGTGACTGGGAAAACCCTGGCCCAAGGTCGTGATCCCGTCTCTTGACC3850.12028393256601558No Hit
GTCGTGACTGGGAAAACCCTGGGTTCGGGTCGTGATATTAACAGCTGACC3820.11934665516939727No Hit
GTCGTGACTGGGAAAACCCTGGATAGTGGTCGTGATGCTAGCCGTTGACC3720.11622239718066958No Hit
GTCGTGACTGGGAAAACCCTGGAATAAGGTCGTGATCAATGTTGATGACC3720.11622239718066958No Hit
GTCGTGACTGGGAAAACCCTGGGAAATGGTCGTGATTCTATGAAATGACC3710.11590997138179683No Hit
GTCGTGACTGGGAAAACCCTGGTGTCCAGTCGTGATCTCAAGACATGACC3670.11466026818630576No Hit
GTCGTGACTGGGAAAACCCTGGCAGTCGGTCGTGATTTTGCTGTATGACC3630.11341056499081469No Hit
GTCGTGACTGGGAAAACCCTGGAGCCGGGTCGTGATTTAGCTAGATGACC3580.11184843599645083No Hit
GTCGTGACTGGGAAAACCCTGGTCGGCCGTCGTGATAAATGTGAATGACC3580.11184843599645083No Hit
GTCGTGACTGGGAAAACCCTGGAATATACCGAGGCCTCTCCAGTCTGACC3580.11184843599645083No Hit
GTCGTGACTGGGAAAACCCTGGGCCAGGGTCGTGATAACCCTTTCTGACC3570.11153601019757808No Hit
GTCGTGACTGGGAAAACCCTGGTTGAATGTCGTGATGTGATATTTTGACC3560.1112235843987053No Hit
GTCGTGACTGGGAAAACCCTGGCCTCGCGTCGTGATATGGCCTCCTGACC3520.10997388120321423No Hit
GTCGTGACTGGGAAAACCCTGGCCCTCTGTCGTGATACCTACGGATGACC3450.10778690061110487No Hit
GTCGTGACTGGGAAAACCCTGGATTGCGGTCGTGATCTTCTCGTTTGACC3400.10622477161674101No Hit
GTCGTGACTGGGAAAACCCTGGTCACACGTCGTGATCCGGAGGATTGACC3390.10591234581786826No Hit
GTCGTGACTGGGAAAACCCTGGTTCTTTGTCGTGATTTTAGCCCTTGACC3380.10559992001899549No Hit
GTCGTGACTGGGAAAACCCTGGCGCCGCGTCGTGATGCAGGGCGCTGACC3370.10528749422012273No Hit
GTCGTGACTGGGAAAACCCTGGCAATATGTCGTGATTTGAGCTTTTGACC3260.10185081043252227No Hit
GTCGTGACTGGGAAAACCCTGGCTAAGGGTCGTGATCCGATGGAGTGACC3240.10122595883477674No Hit
GTCGTGACTGGGAAAACCCTGGCAAGCAGTCGTGATTAGAAGCGCTGACC3230.10091353303590397No Hit
GTCGTGACTGGGAAAACCCTGGGATCTTGTCGTGATACAAGTAAATGACC3230.10091353303590397No Hit
GTCGTGACTGGGAAAACCCTGGATAGACGTCGTGATGGTCATGGCTGACC3220.1006011072370312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACT100.0015277619242.17933
CGTGACT314300.0240.407093
TCGTGAC315250.0240.025682
GTGACTG315650.0239.230264
TGACTGG315650.0239.191935
GACTGGG317400.0237.873116
TGGGAAA317200.0237.641669
ACTGGGA320850.0234.975957
CTGGGAA328900.0229.1888
GACCGGG850.0142.347146
GTCGTGA609950.0124.0354461
TGCGAAA302.5270288E-4120.995079
TCGGACT551.9120671E-7110.133172
TCGTGGC1100.0110.133172
CGGGGAA1000.0108.895589
CGTGGCT1450.0108.563153
ACCGGGA1250.0106.475677
TGACCGG1400.095.067565
TCGAGAC1051.0913936E-1192.302092
CGAGACT1200.090.8172453
Produced by FastQC (version 0.11.5)