FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729203
Sequences flagged as poor quality0
Sequence length248
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT1661650.0241.493563
TGACTGG1666250.0241.456655
GTGACTG1666550.0241.418494
TCGTGAC1668450.0241.122042
GACTGGG1672250.0240.380976
ACTGGGA1667450.0239.781577
CTGGGAA1710400.0234.156628
TGGGAAA1698650.0228.85819
CGTGGCT20850.0219.548633
TCGTGGC19500.0209.998252
GTGACTT8300.0167.740954
GCGTGGC5150.0157.616322
GGTGACT15200.0152.968733
ACTTGGA9600.0150.022667
ACTGGGG15250.0147.612877
TGACTTG10000.0139.19685
GTGGCTG34950.0136.47984
GTCGTGG30650.0135.631551
GTGGTGA18100.0135.260151
GTCGTGA3256700.0123.576511
Produced by FastQC (version 0.11.5)