FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144648
Sequences flagged as poor quality0
Sequence length248
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGACACGAGTCGTGATTAACCAGGGGCCAA36472.521293070073558No Hit
GTCGTGACTGGGAAAACCCTGGGGAGCAGTCGTGATTAAACCTTAGCCAA10760.7438747856866323No Hit
GTCGTGACTGGGAAAACCCTGGAGCCATGTCGTGATGGAGTAAGAGCCAA8020.5544494220452408No Hit
GTCGTGACTGGGAAAACCCTGGAGGGACGTCGTGATTTCACCACTGCCAA5840.4037387312648637No Hit
GTCGTGACTGGGAAAACCCTGGTTTACAGTCGTGATACTGATGGAGCCAA2100.14518002322880372No Hit
GTCGTGACTGGGAAAACCCTGGGAGATCGTCGTGATCGCGATCTCGCCAA1800.12444001991040318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACT201.6364174E-7242.569843
CGAGACT100.0015195474242.569843
CTTACCG151.584728E-5242.317874
CTTACGG100.0015242846242.317864
TCGTGAC124750.0241.557142
GTGACTG124650.0241.443074
TGACTGG124250.0241.07835
CGTGACT120750.0240.761873
GACTGGG125550.0240.523976
TGGGAAA125900.0231.7969
ACTGGGA133150.0225.068137
GTGACCT1750.0221.547784
CTGGGAA135050.0220.839458
TTACCGG550.0220.136525
TCGTGGC1700.0214.255022
CGTGACC1900.0210.652743
TGACGGG3100.0206.999335
GTGACGG3150.0203.854724
GACGGGG4250.0202.196666
CGTGACG2350.0196.120293
Produced by FastQC (version 0.11.5)