FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474371
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGAACAAGGTCGTGATAAGTGCAGAGATCA16900.11462515201397748No Hit
GTCGTGACTGGGAAAACCCTGGCGAACCGTCGTGATCCTGTCACTGATCA16020.10865650504520233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT1373950.0241.471393
TGACTGG1379050.0241.1925
TCGTGAC1380650.0241.001142
GTGACTG1381000.0240.89234
GACTGGG1386950.0239.888586
TCGTGGC34550.0239.503362
CGTGGCT45500.0232.604343
ACTGGGA1449050.0227.564127
TGGGAAA1420900.0227.319969
CTGGGAA1487950.0224.103588
GCGTGGC14650.0195.997122
GTCGTGG44950.0191.978011
ACTGGGG17200.0174.49217
GTGACTT6750.0173.967394
CGTTGCA351.2936653E-8172.989493
GCGTGAC1900.0172.166692
CGTTGCT1450.0167.024323
CGTTACT800.0166.50243
TGGCTGG65650.0163.905385
GGTGACT11850.0163.500613
Produced by FastQC (version 0.11.5)