FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3111467
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCGCACTGTCGTGATCTTTACGCTGATCA102270.3286874005091489No Hit
GTCGTGACTGGGAAAACCCTGGACTAGCGTCGTGATATGTGCGGCGATCA78470.2521961505617768No Hit
GTCGTGACTGGGAAAACCCTGGGTGGAGGTCGTGATGCAGAGTACGATCA78190.25129625350357243No Hit
GTCGTGACTGGGAAAACCCTGGCCGGCTGTCGTGATTGCCGCGGCGATCA76890.24711816001905212No Hit
GTCGTGACTGGGAAAACCCTGGTAATTGGTCGTGATACGTATCTTGATCA73740.23699431811425287No Hit
GTCGTGACTGGGAAAACCCTGGGAAACCGTCGTGATGAATCGGCTGATCA71880.23101643051332377No Hit
GTCGTGACTGGGAAAACCCTGGCGTTCGGTCGTGATCCGATGGCTGATCA66290.21305062852988638No Hit
GTCGTGACTGGGAAAACCCTGGGTAACCGTCGTGATTCTAATAGTGATCA63730.20482299828344636No Hit
GTCGTGACTGGGAAAACCCTGGGCGGTAGTCGTGATCTGAACGCTGATCA63210.2031517608896382No Hit
GTCGTGACTGGGAAAACCCTGGGGAGCTGTCGTGATGTTCTGGTAGATCA63010.2025089772766351No Hit
GTCGTGACTGGGAAAACCCTGGCAGCTTGTCGTGATTCCAACCAGGATCA61390.1973024300113098No Hit
GTCGTGACTGGGAAAACCCTGGCCATCAGTCGTGATGCTGTTTTAGATCA60760.19527766163034993No Hit
GTCGTGACTGGGAAAACCCTGGTCGTTAGTCGTGATGCCGAGTCTGATCA60390.19408851194629415No Hit
GTCGTGACTGGGAAAACCCTGGGTTACAGTCGTGATGCCTGTGTGGATCA59140.1900711143650246No Hit
GTCGTGACTGGGAAAACCCTGGACATCTGTCGTGATTGTCTACCTGATCA58280.18730714482911115No Hit
GTCGTGACTGGGAAAACCCTGGCAGCTAGTCGTGATTAGAGGTACGATCA58030.18650366531285725No Hit
GTCGTGACTGGGAAAACCCTGGGCTAGGGTCGTGATGATAACCCCGATCA57830.18586088169985412No Hit
GTCGTGACTGGGAAAACCCTGGCGGTTGGTCGTGATCATGAGGGCGATCA56410.18129711804753193No Hit
GTCGTGACTGGGAAAACCCTGGCCAAATGTCGTGATCCAGACTATGATCA56030.180075829182826No Hit
GTCGTGACTGGGAAAACCCTGGCTACGGGTCGTGATCGAGGGGTCGATCA54990.1767333543952097No Hit
GTCGTGACTGGGAAAACCCTGGGGGTCCGTCGTGATCTAGAGGATGATCA54910.17647624095000847No Hit
GTCGTGACTGGGAAAACCCTGGGCGTTCGTCGTGATCATAAGTATGATCA54820.17618698832415708No Hit
GTCGTGACTGGGAAAACCCTGGAATATCGTCGTGATTATATGGCTGATCA54380.17477286437555017No Hit
GTCGTGACTGGGAAAACCCTGGTGTTGCGTCGTGATTAGGAGGGAGATCA52710.16940562120697406No Hit
GTCGTGACTGGGAAAACCCTGGGCTTTGGTCGTGATTTGAACTGAGATCA52160.16763796627121547No Hit
GTCGTGACTGGGAAAACCCTGGTTAGATGTCGTGATTATCGCACTGATCA51010.1639419604964475No Hit
GTCGTGACTGGGAAAACCCTGGCTACCCGTCGTGATTGACTGGGGGATCA50830.1633634552447447No Hit
GTCGTGACTGGGAAAACCCTGGCATGATGTCGTGATTCGGCGTATGATCA47670.15320747415929528No Hit
GTCGTGACTGGGAAAACCCTGGTGTGTAGTCGTGATCAAGGCGGGGATCA47350.15217902037849027No Hit
GTCGTGACTGGGAAAACCCTGGGCCATTGTCGTGATCCTGATAAAGATCA46970.15095773151378433No Hit
GTCGTGACTGGGAAAACCCTGGCACCTGGTCGTGATCTTTCTAAAGATCA46860.15060420052663262No Hit
GTCGTGACTGGGAAAACCCTGGTCTGTCGTCGTGATACAAATTGAGATCA45910.14755097836486777No Hit
GTCGTGACTGGGAAAACCCTGGATTGGTGTCGTGATAGGGTACCCGATCA45710.14690819475186465No Hit
GTCGTGACTGGGAAAACCCTGGGGACTGGTCGTGATAGCCTGGGAGATCA45540.146361828680812No Hit
GTCGTGACTGGGAAAACCCTGGCGAGAAGTCGTGATCCCAGGGCTGATCA45320.14565476670650854No Hit
GTCGTGACTGGGAAAACCCTGGCTTTAAGTCGTGATGAAGACCCAGATCA45260.1454619316226076No Hit
GTCGTGACTGGGAAAACCCTGGGCGCAGGTCGTGATACCATGTATGATCA45230.14536551408065715No Hit
GTCGTGACTGGGAAAACCCTGGACTTCTGTCGTGATTACGTGTTGGATCA44760.14385497259009977No Hit
GTCGTGACTGGGAAAACCCTGGCATCGAGTCGTGATGGAGGCGGTGATCA44540.14314791061579635No Hit
GTCGTGACTGGGAAAACCCTGGATTTCGGTCGTGATAAGGACTGGGATCA44530.14311577143514617No Hit
GTCGTGACTGGGAAAACCCTGGACCAGCGTCGTGATCGCGGAGACGATCA44340.14250512700279322No Hit
GTCGTGACTGGGAAAACCCTGGGTGTGCGTCGTGATCATGAAAAAGATCA44300.14237657028019257No Hit
GTCGTGACTGGGAAAACCCTGGCGATAAGTCGTGATTTAAGCAAAGATCA43760.14064105452508416No Hit
GTCGTGACTGGGAAAACCCTGGCCAAAAGTCGTGATTATTAAGATGATCA43620.14019110599598197No Hit
GTCGTGACTGGGAAAACCCTGGCACCGAGTCGTGATAAAGATCCCGATCA43090.13848772942152365No Hit
GTCGTGACTGGGAAAACCCTGGAGCCACGTCGTGATGACCGAGTAGATCA43030.13829489433762274No Hit
GTCGTGACTGGGAAAACCCTGGCCAGGGGTCGTGATGCATAGATTGATCA42980.13813419843437194No Hit
GTCGTGACTGGGAAAACCCTGGAGCCCTGTCGTGATAATTTGCAGGATCA42880.13781280662787038No Hit
GTCGTGACTGGGAAAACCCTGGTAGCGTGTCGTGATGGCGATACCGATCA42540.13672007448576506No Hit
GTCGTGACTGGGAAAACCCTGGTGCTCCGTCGTGATTCATGAGGGGATCA42520.13665579612446477No Hit
GTCGTGACTGGGAAAACCCTGGCACAGTGTCGTGATGTGAGTGGTGATCA42490.1365593785825143No Hit
GTCGTGACTGGGAAAACCCTGGTACATAGTCGTGATTTGGATTATGATCA42150.13546664644040898No Hit
GTCGTGACTGGGAAAACCCTGGTATCATGTCGTGATGAGGATACTGATCA42060.13517739381455757No Hit
GTCGTGACTGGGAAAACCCTGGTAGTATGTCGTGATGAGCAGTGTGATCA41980.13492028036935633No Hit
GTCGTGACTGGGAAAACCCTGGGACTGCGTCGTGATTCAAGGGGGGATCA41700.13402038331115193No Hit
GTCGTGACTGGGAAAACCCTGGGAGCTAGTCGTGATGCGTAGACGGATCA41580.13363471314335007No Hit
GTCGTGACTGGGAAAACCCTGGAAGCGCGTCGTGATATCAAGAGAGATCA41420.13312048625294756No Hit
GTCGTGACTGGGAAAACCCTGGTGCCGTGTCGTATTGAGTATCTCGATCA41060.13196347574954193No Hit
GTCGTGACTGGGAAAACCCTGGAGCAGTGTCGTGATGACTGACTCGATCA40940.13157780558174006No Hit
GTCGTGACTGGGAAAACCCTGGGTTCTTGTCGTGATGTACTTCCAGATCA40790.1310957178719877No Hit
GTCGTGACTGGGAAAACCCTGGGGACTCGTCTGAGATGTGGGTACGATCA40680.130742186884836No Hit
GTCGTGACTGGGAAAACCCTGGGTCCGTGTCGTGATCTGTACGAGGATCA40570.13038865589768425No Hit
GTCGTGACTGGGAAAACCCTGGCCCGCGGTCGTGATAACATGTTGGATCA39970.1284603050586749No Hit
GTCGTGACTGGGAAAACCCTGGGAGTCAGTCGTGATCATGTATTGGATCA39930.12833174833607428No Hit
GTCGTGACTGGGAAAACCCTGGGCATCGGTCGATGTGCAGACACAGATCA39850.128074634890873No Hit
GTCGTGACTGGGAAAACCCTGGCAGTGTGTCGTGATAGTAACGTGGATCA39690.12756040800047053No Hit
GTCGTGACTGGGAAAACCCTGGTATGAACCATGAGTATATGTCCTGATCA39420.1266926501229163No Hit
GTCGTGACTGGGAAAACCCTGGTTTCGTGTCGTGATTGGTAAGTGGATCA39320.12637125831641474No Hit
GTCGTGACTGGGAAAACCCTGGGGGAATGTCGTGATCCCAAGTACGATCA39270.12621056241316395No Hit
GTCGTGACTGGGAAAACCCTGGTAGTGAGTCGTGATGAATAGAAGGATCA39000.12534280453560973No Hit
GTCGTGACTGGGAAAACCCTGGAGATGCGTCGTGATATAAAGCACGATCA38730.1244750466580555No Hit
GTCGTGACTGGGAAAACCCTGGAGTGTTGTCGTGATTGTCGGTTAGATCA38630.12415365485155395No Hit
GTCGTGACTGGGAAAACCCTGGTTACCCGTCGTGATTGCAAGTATGATCA38580.12399295894830317No Hit
GTCGTGACTGGGAAAACCCTGGATCAAGGTCGTGATGGAAATGATGATCA38400.12341445369660035No Hit
GTCGTGACTGGGAAAACCCTGGGCTTCTGTCGTGATTTTTATAATGATCA38220.12283594844489754No Hit
GTCGTGACTGGGAAAACCCTGGTCCCACGTCGTGATACGCGTCATGATCA37750.12132540695434019No Hit
GTCGTGACTGGGAAAACCCTGGGTTATAGTCGTGATCAAAAGTAGGATCA37640.12097187596718847No Hit
GTCGTGACTGGGAAAACCCTGGTATCGCGTCGTGATACGTGTCGCGATCA36970.118818550863628No Hit
GTCGTGACTGGGAAAACCCTGGCCTTCTGTCGTGATAGGAATAGAGATCA36580.1175651228182719No Hit
GTCGTGACTGGGAAAACCCTGGATACACGTCGTGATGCAATGGGAGATCA36530.11740442691502112No Hit
GTCGTGACTGGGAAAACCCTGGATCGCGGTCGTGATCACTGATGAGATCA36350.1168259216633183No Hit
GTCGTGACTGGGAAAACCCTGGCCCGTCGTCGTGATGCCGAGCTAGATCA36190.11631169477291581No Hit
GTCGTGACTGGGAAAACCCTGGTCGCAAGTCGTGATCCTGAGAGGGATCA36050.11586174624381362No Hit
GTCGTGACTGGGAAAACCCTGGCCGATAGTCGTGATACAAATCGAGATCA36020.11576532870186314No Hit
GTCGTGACTGGGAAAACCCTGGTGCAGTGTCGTGATACAAGAGTGGATCA36010.11573318952121299No Hit
GTCGTGACTGGGAAAACCCTGGTCGTAAGTCGTGATATGGGCATAGATCA35940.11550821525666188No Hit
GTCGTGACTGGGAAAACCCTGGCCGAGCGTCGTGATATCTGCCTTGATCA35790.11502612754690955No Hit
GTCGTGACTGGGAAAACCCTGGTTACCCGTCGTGATTGAAATGAAGATCA35680.11467259655975781No Hit
GTCGTGACTGGGAAAACCCTGGCCGTTCGTCGTGATATGCACGGGGATCA35650.11457617901780735No Hit
GTCGTGACTGGGAAAACCCTGGCATCCTGTCGTGATCAACCTCGGGATCA35610.11444762229520673No Hit
GTCGTGACTGGGAAAACCCTGGCAAATAGTCGTGATAGACCCCAGGATCA35530.11419050885000548No Hit
GTCGTGACTGGGAAAACCCTGGCCAACAGTCTGGCCGGTGGTCGTGATCA35010.11251927145619735No Hit
GTCGTGACTGGGAAAACCCTGGACTTTAGTCGTGATTCCGAGTATGATCA34910.11219787964969578No Hit
GTCGTGACTGGGAAAACCCTGGGCACCAGTCGTGATTGCACCACCGATCA34890.11213360128839546No Hit
GTCGTGACTGGGAAAACCCTGGACCATCGTCTGATTTCCGCAGGGGATCA34720.11158723521734282No Hit
GTCGTGACTGGGAAAACCCTGGCAAGTTGTCGTGATTTGATTGGCGATCA34630.1112979825914914No Hit
GTCGTGACTGGGAAAACCCTGGAGGCTCGTCGTGATTACAATGGAGATCA34420.11062305979783811No Hit
GTCGTGACTGGGAAAACCCTGGACCCATGTCGTGAATGGAGAGGCGATCA34210.10994813700418485No Hit
GTCGTGACTGGGAAAACCCTGGTGACCGGTCGTGATGGGGCCAGCGATCA34170.10981958028158421No Hit
GTCGTGACTGGGAAAACCCTGGTAAGTAGTCGTGATTCGCAGAGAGATCA34080.10953032765573283No Hit
GTCGTGACTGGGAAAACCCTGGTCGTACGTCGTGATCTCAATGGGGATCA34030.10936963175248203No Hit
GTCGTGACTGGGAAAACCCTGGTTATGAGTCGTGATAGCGAGTAAGATCA33650.10814834288777608No Hit
GTCGTGACTGGGAAAACCCTGGCTATAGGTCGTGATTCTTATGAAGATCA33460.10753769845542312No Hit
GTCGTGACTGGGAAAACCCTGGGCCCGTGTCGTGATAGTGCCAGCGATCA33410.10737700255217233No Hit
GTCGTGACTGGGAAAACCCTGGATAACAGTCGTGATTAGGTCTCAGATCA33400.10734486337152219No Hit
GTCGTGACTGGGAAAACCCTGGGTTTACGTCGTGATCCGTCGACAGATCA33330.10711988910697108No Hit
GTCGTGACTGGGAAAACCCTGGATGAAACATCCTGAGGACAGTGCGATCA33220.10676635811981937No Hit
GTCGTGACTGGGAAAACCCTGGTAACAGGTCGTGATGATTCTATCGATCA33120.1064449663133178No Hit
GTCGTGACTGGGAAAACCCTGGCGAGTGGTCGTGATCCATCGGGAGATCA33060.10625213122941687No Hit
GTCGTGACTGGGAAAACCCTGGTTGATTGTCGTGATGGGTGCCCTGATCA33040.10618785286811655No Hit
GTCGTGACTGGGAAAACCCTGGCCCCCAGTCGTGATGAGCGCACGGATCA32910.10577004351966451No Hit
GTCGTGACTGGGAAAACCCTGGGGGCTTGTCGTGATGCATAGAGAGATCA32820.10548079089381313No Hit
GTCGTGACTGGGAAAACCCTGGGAGGGTGTCGTGATCTGCTGCTTGATCA32660.10496656400341062No Hit
GTCGTGACTGGGAAAACCCTGGAGGGTTGTCGTGATTTCGATTTTGATCA32510.10448447629365827No Hit
GTCGTGACTGGGAAAACCCTGGCTAAGCGTCGTGATCACACCACCGATCA32330.10390597104195545No Hit
GTCGTGACTGGGAAAACCCTGGACGCCCGTCGTGATCGTAACTTGGATCA32060.10303821316440123No Hit
GTCGTGACTGGGAAAACCCTGGGCCCTGGTCGTGATACTAATGCCGATCA31790.102170455286847No Hit
GTCGTGACTGGGAAAACCCTGGTTCATAGTCGTGATCTTAGATCTGATCA31700.1018812026609956No Hit
GTCGTGACTGGGAAAACCCTGGACCGGGTCTGATTGGAGATTCACGATCA31470.101142001506042No Hit
GTCGTGACTGGGAAAACCCTGGCGAGTTGTCGTGATCCTCCCGACGATCA31460.10110986232539185No Hit
GTCGTGACTGGGAAAACCCTGGTAGCAAGTCGTGATAAACTGTATGATCA31340.10072419215758996No Hit
GTCGTGACTGGGAAAACCCTGGAGTCTAGTCGTGATTCCTAATTTGATCA31150.100113547725237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT2777650.0241.668983
GTGACTG2861200.0241.315724
TGACTGG2869250.0240.571845
GACTGGG2897000.0240.44486
TCGTGAC2840150.0240.019942
ACTGGGA2879900.0239.467157
CTGGGAA2940750.0233.19998
TGGGAAA2886750.0232.807749
GGTGACT87500.0221.402253
TGACGGG51950.0219.313925
GACGGGG59500.0218.503656
CGTGGCT38800.0215.456193
GCGTGAC21500.0214.275922
TCGTGGC35300.0209.499682
CGTGACG36900.0201.596793
CGTGACA40500.0197.737053
GTGGTGA128100.0192.024611
GTGACGG59450.0190.874394
ACGGGGA64400.0187.189717
TGGTGAC127450.0183.588612
Produced by FastQC (version 0.11.5)