FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967174
Sequences flagged as poor quality0
Sequence length248
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA1867800.0241.361657
TGACTGG1882850.0240.980015
GTGACTG1884400.0240.797164
TCGTGAC1887900.0240.352482
TGGGAAA1825600.0240.026879
GACTGGG1892650.0239.631736
CGTGACT1899100.0238.360813
CTGGGAA1920700.0235.911278
CGTGGCT34250.0207.604683
TGGGAAC52150.0192.385889
GTGACTT8500.0188.03464
TGCGAAA1350.0170.330649
TCGTGGC38450.0156.657712
GTGACAG6400.0155.137474
TCGGGAC6350.0148.871872
TCGTGCC1950.0142.950262
CGTGACA6600.0141.320893
ACTGGGG20300.0141.29817
CTGCGAA1650.0139.364978
TGACAGG7500.0138.795825
Produced by FastQC (version 0.11.5)