FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792095
Sequences flagged as poor quality0
Sequence length248
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTTGCCTGTCGTGATAGCAAGTTTTTAGG25730.3248347736067012No Hit
GTCGTGACTGGGAAAACCCTGGCCTATAGTCGTGATGTCAAGTCATTAGG24750.31246252027850197No Hit
GTCGTGACTGGGAAAACCCTGGACAAGGGTCGTGATAGTATACCCTTAGG24250.30615014613146146No Hit
GTCGTGACTGGGAAAACCCTGGTGTTTTGTCGTGATCAAATACTTTTAGG23270.2937778928032622No Hit
GTCGTGACTGGGAAAACCCTGGCCTAACGGTCGTGATAACAATGGTTAGG22870.2887279934856299No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTGCCTAAAAAAAAAGTTAGG20510.258933587511599No Hit
GTCGTGACTGGGAAAACCCTGGTATCCCGTCGTGATCAGAGTGATTTAGG19330.2440363845245835No Hit
GTCGTGACTGGGAAAACCCTGGCACCTCGTCGTGATACGTATGATTTAGG18630.23519906071872693No Hit
GTCGTGACTGGGAAAACCCTGGACATCCGTCGTGATGGACATCCGTTAGG18510.2336840909234372No Hit
GTCGTGACTGGGAAAACCCTGGAGGACGGTCGTGATCTGGATGGTTTAGG17050.21525195841407913No Hit
GTCGTGACTGGGAAAACCCTGGAGTACAGTCGTGATTGAACCCCGTTAGG15260.19265365896767433No Hit
GTCGTGACTGGGAAAACCCTGGGTCCACGTCGTGATAACAAGAGTTTAGG14840.18735126468416036No Hit
GTCGTGACTGGGAAAACCCTGGATGACGGTCGTGATGGTAGTTAGTTAGG14480.18280635529829126No Hit
GTCGTGACTGGGAAAACCCTGGCCTGGCGTCGTGATTAGAAGTATTTAGG13900.17548400128772432No Hit
GTCGTGACTGGGAAAACCCTGGGCACTAGTCGGATGAGAATGACATTAGG13560.1711915868677368No Hit
GTCGTGACTGGGAAAACCCTGGCCCCACGTCGTGATAGAAGCTACTTAGG13480.17018160700421034No Hit
GTCGTGACTGGGAAAACCCTGGTTCAGTGTCGTGATTGAAAGTTGTTAGG12760.1610917882324721No Hit
GTCGTGACTGGGAAAACCCTGGTTGGCCGTCGTGATCAATGAGTGTTAGG12440.15705186877836624No Hit
GTCGTGACTGGGAAAACCCTGGGAGCCAGTCGTGATATTTAGTACTTAGG12380.1562943838807214No Hit
GTCGTGACTGGGAAAACCCTGGTCCGCCGTCGTGATCATAGTAGTTTAGG12360.15604188891483975No Hit
GTCGTGACTGGGAAAACCCTGGTAAGATGTCGTGATAATTCGGTGTTAGG12350.15591564143189895No Hit
GTCGTGACTGGGAAAACCCTGGCGACTTGTCGTGATGTGTAGGACTTCGG12210.15414817667072764No Hit
GTCGTGACTGGGAAAACCCTGGGGTGACGTCGTGATTAAGTCGGCTTAGG12080.15250695939249712No Hit
GTCGTGACTGGGAAAACCCTGGACATATGTCGTGATGTTGGGTACTTAGG12070.15238071190955632No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTGCCTAAACCACAAGTTAGG11960.15099198959720742No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGGGTCGTGATCCAACCCAGTTAGG11690.14758330755780558No Hit
GTCGTGACTGGGAAAACCCTGGCACCAAGTCGTGTAACGACTGCTTTAGG11270.1422809132742916No Hit
GTCGTGACTGGGAAAACCCTGGTTTGAAGTCGTGATCGAGGCACTTTAGG10940.1381147463372449No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTGCCTAAACAACAAGTTAGG10220.12902492756550665No Hit
GTCGTGACTGGGAAAACCCTGGCACTCCGTCGTGATCTTCCTGTCCAAGG9970.12586874049198643No Hit
GTCGTGACTGGGAAAACCCTGGTTTGCGGTCGTGATGCTCGTATTTTAGG9840.12422752321375594No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTGCCTAAAAAACAAGTTAGG9620.12145007858905812No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTGCCTAAACAAAAAGTTAGG9280.11715766416907064No Hit
GTCGTGACTGGGAAAACCCTGGGGTTCGGTCGTGATGGCTTTGAGTTAGG9240.11665267423730741No Hit
GTCGTGACTGGGAAAACCCTGGAGACAGGTCGTGATGCATATCGGTTAGG9200.11614768430554415No Hit
GTCGTGACTGGGAAAACCCTGGTAACCCGTCGTGATGGATGTACATTAGG9150.11551644689084012No Hit
GTCGTGACTGGGAAAACCCTGGTTAAGCGTCGTGATACCACCACGTTAGG9100.11488520947613608No Hit
GTCGTGACTGGGAAAACCCTGGATGTACGTCGTGATTAGTACCCCTTAGG9010.1137489821296688No Hit
GTCGTGACTGGGAAAACCCTGGGGAGTGGTCGTGATCCATTCTTATTAGG8990.11349648716378717No Hit
GTCGTGACTGGGAAAACCCTGGATATCAGTCGTGATTCAAAAACGTTAGG8980.11337023968084636No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTGCCTAAGGGGGGAGTTAGG8900.1123602598173199No Hit
GTCGTGACTGGGAAAACCCTGGTTATTAGTCGTGATGCGCAGTGATTAGG8760.11059279505614857No Hit
GTCGTGACTGGGAAAACCCTGGAAACGGGTCGTGATTAAACGGGCTTAGG8300.10478541084087137No Hit
GTCGTGACTGGGAAAACCCTGGAAAGGGGTCGTGATAGCTATCCCTTAGG8290.10465916335793055No Hit
GTCGTGACTGGGAAAACCCTGGTAGAAGGTCGTGATGAAGAAGGGTTAGG8110.102386708664996No Hit
GTCGTGACTGGGAAAACCCTGGCCAAAAGTCGTGATTAGCTGGGCTTAGG8030.10137672880146953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC735150.0241.878522
TGGGAAA720150.0240.071879
ACTGGGA737550.0240.021367
CGTGACT737300.0238.026953
CTGGGAA746050.0236.296958
TGACTGG764300.0232.879965
GTGACTG767100.0232.845524
GACTGGG772800.0230.825426
CGTGACA9150.0229.122023
CGTGACG7900.0214.754753
GACGGGG13250.0198.277736
ACGGGGA12500.0196.589927
TCGGGAC5100.0194.941932
TCGAGAC1450.0192.318652
GTGACGG12400.0190.509874
GTGACAG11450.0188.32984
GTGGTGA24400.0179.43881
CGTGGCT8000.0178.744983
ACGTGAC2150.0174.817472
GGTGACT19700.0171.009553
Produced by FastQC (version 0.11.5)