FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380592
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGGAAGTGTCGTGATGGAAAGACATTAGG18810.49423004161937195No Hit
GTCGTGACTGGGAAAACCCTGGTCGACCGTGTGACATCACCTGGGTTAGG14410.3786206751587001No Hit
GTCGTGACTGGGAAAACCCTGGTCTGTGGTCGTGATCTACATAAATTAGG12600.331063185773742No Hit
GTCGTGACTGGGAAAACCCTGGCCAATCGTCGTGATATGCTCTCTTTAGG10530.2766742338251986No Hit
GTCGTGACTGGGAAAACCCTGGCTACGAGTCGTGATACGAATGAGTTAGG9940.2611720687770631No Hit
GTCGTGACTGGGAAAACCCTGGAACACAGTCGTGATTATGTCTTCTTAGG9460.24856013789044434No Hit
GTCGTGACTGGGAAAACCCTGGGTATACGTCGTGATGGCAGGAGCTTAGG9230.24251692100727287No Hit
GTCGTGACTGGGAAAACCCTGGTTAAGGGTCGTGATATCATTATTTTAGG9120.23962668684575608No Hit
GTCGTGACTGGGAAAACCCTGGGCTCCCGTCGTGATGCCGCGGGCTTAGG8330.2188695505948627No Hit
GTCGTGACTGGGAAAACCCTGGTGTCTAGTCGTGATCGAGTTTGATTAGG8250.2167675621137596No Hit
GTCGTGACTGGGAAAACCCTGGCCTCACGTCGTGATTTATACTTATTAGG8120.21335183083196707No Hit
GTCGTGACTGGGAAAACCCTGGCGTGTGGTCGTGATTGTACTGAGTTAGG8090.21256358515155338No Hit
GTCGTGACTGGGAAAACCCTGGTGTTTGGTCGTGATTGCCGTGAGTTAGG8080.2123008365914155No Hit
GTCGTGACTGGGAAAACCCTGGGACTCCGTCGTGATTCGTGCTACTTAGG8070.2120380880312776No Hit
GTCGTGACTGGGAAAACCCTGGCTCGTTGTCGTGATGGCCACGGATTAGG8000.21019884811031234No Hit
GTCGTGACTGGGAAAACCCTGGACACTAGTCGTGATGAGTACTACTTAGG7880.20704586538865766No Hit
GTCGTGACTGGGAAAACCCTGGCCGGAGGTCGTGATGCAAGCGCATTAGG7500.19706142010341784No Hit
GTCGTGACTGGGAAAACCCTGGTTCCTCGTCGTGATTACTTGTAATTAGG7450.19574767730272838No Hit
GTCGTGACTGGGAAAACCCTGGTGATAAGTCGTGATTAGACCCCCTTAGG7270.19101820322024637No Hit
GTCGTGACTGGGAAAACCCTGGGCTTCGGTCGTGATGAGTAAGATTTAGG7090.18628872913776434No Hit
GTCGTGACTGGGAAAACCCTGGTCGTCTGTCGTGATGTTATGTTATTAGG6850.17998276369445496No Hit
GTCGTGACTGGGAAAACCCTGGCTGTTTGTCGTGATAACGACACCTTAGG6840.17972001513431707No Hit
GTCGTGACTGGGAAAACCCTGGCGAAGTGTCGTGATCCAAAGTACTTAGG6660.17499054105183504No Hit
GTCGTGACTGGGAAAACCCTGGTTCATGTCCAGTCCCCCGGCCAATTAGG6600.1734140496910077No Hit
GTCGTGACTGGGAAAACCCTGGAACACCGTCGTGATGCCGAGTTGTTAGG6440.16921007272880145No Hit
GTCGTGACTGGGAAAACCCTGGTAAATTGTCGTGATGCTTAGGGGTTAGG6210.16316685584562995No Hit
GTCGTGACTGGGAAAACCCTGGGCGAGCGTCGTGATATACCACCTTTAGG6170.1621158616050784No Hit
GTCGTGACTGGGAAAACCCTGGATGTTGCCCTCCAGCTGCACATGTTAGG6020.15817463320301003No Hit
GTCGTGACTGGGAAAACCCTGGAATCCTGTCGTGATCGAACCCTCTTAGG5990.15738638752259637No Hit
GTCGTGACTGGGAAAACCCTGGTCGGTTGTCGTGATGCCATGTTTTTAGG5980.15712363896245848No Hit
GTCGTGACTGGGAAAACCCTGGGCGTTGGTCGTGATTGGGACGAGTTAGG5960.1565981418421827No Hit
GTCGTGACTGGGAAAACCCTGGTTCCCCGTCGTGATTGCACTCCGTTAGG5790.15213141631983856No Hit
GTCGTGACTGGGAAAACCCTGGATACTCGTCGTGATGTTAACCTCTTAGG5750.151080422079287No Hit
GTCGTGACTGGGAAAACCCTGGCAGTCCGTCGTGATCCGTGATCTTTAGG5620.14766469079749442No Hit
GTCGTGACTGGGAAAACCCTGGCCAGATGTCGTGATTAGTATACCTTAGG5610.14740194223735653No Hit
GTCGTGACTGGGAAAACCCTGGACGTATGTCGTGATTAACAGCTCTTAGG5450.1431979652751503No Hit
GTCGTGACTGGGAAAACCCTGGACTGGTGTCGTGATGCGTAACCCTTAGG5400.14188422247446084No Hit
GTCGTGACTGGGAAAACCCTGGCTGCAAGTCGTGATGGCACTAAATTAGG5360.14083322823390929No Hit
GTCGTGACTGGGAAAACCCTGGGGCCTGGTCGTGATTACAAGACATTAGG5300.13925673687308193No Hit
GTCGTGACTGGGAAAACCCTGGAGACCCGTCGTGATTGTTGTGACTTAGG5280.13873123975280616No Hit
GTCGTGACTGGGAAAACCCTGGAGTGTTGTCGTGATACAAACACTTTAGG5210.13689199983184092No Hit
GTCGTGACTGGGAAAACCCTGGTTACTAGTCGTGATTTAGCAGACTTAGG5190.13636650271156514No Hit
GTCGTGACTGGGAAAACCCTGGGGATCAGTCGTGATAGGTAAAGTTTAGG5110.134264514230462No Hit
GTCGTGACTGGGAAAACCCTGGTTTACGGTCGTGATCCGTTGTGATTAGG5060.13295077142977257No Hit
GTCGTGACTGGGAAAACCCTGGACCTAAGTCGTGATTTCCAAGAGTTAGG5010.1316370286290831No Hit
GTCGTGACTGGGAAAACCCTGGGAGATGGTCGTGATGACGATAGTTTAGG4960.13032328582839367No Hit
GTCGTGACTGGGAAAACCCTGGTTCCCTGTCGTGATGAGGGTAGCTTAGG4900.12874679446756632No Hit
GTCGTGACTGGGAAAACCCTGGTCATGTGTCGTGATTAAATGATATTAGG4890.12848404590742843No Hit
GTCGTGACTGGGAAAACCCTGGTTCTACGTCGTGATAATCTCACATTAGG4880.12822129734729054No Hit
GTCGTGACTGGGAAAACCCTGGTTGAACGTCGTGATTCGAAGTTATTAGG4780.12559381174591164No Hit
GTCGTGACTGGGAAAACCCTGGGTGATTGTCGTGATTAGGCCGTATTAGG4760.12506831462563583No Hit
GTCGTGACTGGGAAAACCCTGGCCCTCCGTCGTGATTCTCGTCCCTTAGG4690.12322907470467062No Hit
GTCGTGACTGGGAAAACCCTGGCCGCAAGTCGTGATACGAAGATTTTAGG4690.12322907470467062No Hit
GTCGTGACTGGGAAAACCCTGGAGCGATGTCGTGATCATCAACTGTTAGG4610.12112708622356749No Hit
GTCGTGACTGGGAAAACCCTGGAAGGTGGTCGTGATGTGGACACATTAGG4580.12033884054315383No Hit
GTCGTGACTGGGAAAACCCTGGTTTAATGTCGTGATTAGAACGAATTAGG4570.12007609198301593No Hit
GTCGTGACTGGGAAAACCCTGGTGAGTGGTCGTGATCGCAAACCCTTAGG4540.11928784630260225No Hit
GTCGTGACTGGGAAAACCCTGGTCTGTCGTCGTGATATATCCTCATTAGG4540.11928784630260225No Hit
GTCGTGACTGGGAAAACCCTGGGACATGGTCGTGATACTCCACGCTTAGG4520.11876234918232649No Hit
GTCGTGACTGGGAAAACCCTGGTGCAACGTCGTGATGATTACTCCTTAGG4410.11587211502080967No Hit
GTCGTGACTGGGAAAACCCTGGGTGTCAGTCGTGATGATGTTCCGTTAGG4400.1156093664606718No Hit
GTCGTGACTGGGAAAACCCTGGCCGAGTGTCGTGATGCATTGTTATTAGG4400.1156093664606718No Hit
GTCGTGACTGGGAAAACCCTGGGATTTTAGTCGTGATAAACATTTTTAGG4320.11350737797956867No Hit
GTCGTGACTGGGAAAACCCTGGGCCGGTGTCGTGATAGTGTATACTTAGG4320.11350737797956867No Hit
GTCGTGACTGGGAAAACCCTGGTTTCCAGTCGTGATTGCGAAACCTTAGG4320.11350737797956867No Hit
GTCGTGACTGGGAAAACCCTGGGGTCCCGTCGTGATCTTTGCCCCTTAGG4300.11298188085929288No Hit
GTCGTGACTGGGAAAACCCTGGTCGACCGTCTGACATCACCTGGGTTAGG4280.11245638373901712No Hit
GTCGTGACTGGGAAAACCCTGGTCCCGAGTCGTGATCCTCGCACTTTAGG4280.11245638373901712No Hit
GTCGTGACTGGGAAAACCCTGGTGAAGTGTCGTGATGTTCATTATTTAGG4260.11193088661874133No Hit
GTCGTGACTGGGAAAACCCTGGAGGGGTGTCGTGATACGGCATAGTTAGG4240.11140538949846554No Hit
GTCGTGACTGGGAAAACCCTGTTATGCGTCGTGATGAAGGTTATATTAGG4230.11114264093832767No Hit
GTCGTGACTGGGAAAACCCTGGTTCGCTGTCGTGATGACCGGGAATTAGG4160.10930340101736243No Hit
GTCGTGACTGGGAAAACCCTGGAAAGGCGTCGTGATCGCTACGCTTTAGG4160.10930340101736243No Hit
GTCGTGACTGGGAAAACCCTGGCCAGGAGTTCGTGATTCGACGGTTTAGG4100.10772690965653509No Hit
GTCGTGACTGGGAAAACCCTGGCCTCCCGTCGTGATAGCACTCCTTTAGG4040.10615041829570775No Hit
GTCGTGACTGGGAAAACCCTGGTAGTAAGTCGTGATTGACTAACTTTAGG4000.10509942405515617No Hit
GTCGTGACTGGGAAAACCCTGGGCGGGTCCTACATTCCTGATATCTTAGG3870.10168369277336359No Hit
GTCGTGACTGGGAAAACCCTGGTGTGTGTCGTGATTTTACGACCCTTAGG3830.10063269853281204No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC353650.0241.391422
ACTGGGA352150.0239.831867
CGTGACT356250.0239.194933
TGGGAAA347400.0238.754879
CTGGGAA363300.0235.137158
TGACTGG374300.0227.577585
GACTGGG374850.0226.923116
GTGACTG375900.0226.797414
CGTGGCT13350.0216.942473
TCGTGGC11300.0203.833052
TCGAGAC204.9872415E-5181.840532
GTCGTGG13250.0178.456561
ACGGGGA351.2933015E-8172.953787
CGTTACT301.2421733E-6161.572223
GGCGTGG5500.0154.329571
CGTGACA1450.0150.42933
GCGTGGC5550.0146.346152
TAGTGAC251.21358185E-4145.472432
GCTGGGG10900.0141.060477
GTCGTGA669450.0127.788771
Produced by FastQC (version 0.11.5)