FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3413071
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT3360800.0241.298693
TGACTGG3363750.0241.175955
TCGTGAC3367450.0241.042882
GTGACTG3365150.0241.031054
ACTGGGA3376750.0239.724497
GACTGGG3380050.0239.612036
CTGGGAA3489050.0231.981038
TGGGAAA3492400.0231.748119
ACGTGAC5950.0150.6552
ACCGGGA6800.0140.569277
GTCGTGA6575450.0123.42821
CGAGACT9450.0112.743213
TGCGAAA4300.0109.7376569
TCGAGAC9800.0107.5382
CGTGGCT12350.0101.9543843
GACGTGA8800.0101.912651
TCGGACT4400.096.35722
ACGGGAA5800.087.6180347
TGACCGG12000.086.717865
CGTTACT3650.082.925233
Produced by FastQC (version 0.11.5)