FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15274
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCCGGTCGTCGTGATATACTTTGCCGAAT1040.6808956396490768No Hit
GTCGTGACTGGGAAAACCCTGGATAACCGTCGTGATAATTTAGCACGAAT910.5957836846929423No Hit
GTCGTGACTGGGAAAACCCTGGCATTCTGTCGTGATCACCCGTGCCGAAT850.5565012439439571No Hit
GTCGTGACTGGGAAAACCCTGGTACGCAGTCGTGATTCAACAGGCCGATG810.5303129501113003No Hit
GTCGTGACTGGGAAAACCCTGGCCTGTTGTCGTGATGTCAGTTGCCGAAT700.458295142071494No Hit
GTCGTGACTGGGAAAACCCTGGTCTGCCGTCGTGATGCTCAGGACCAATG670.4386539216970014No Hit
GTCGTGACTGGGAAAACCCTGGTGCCGAGTCGTGATCCGGATGGCCGAAT660.4321068482388372No Hit
GTCGTGACTGGGAAAACCCTGGCGGGGCGTCGTGATAGAATCGGACGAAT620.4059185544061804No Hit
GTCGTGACTGGGAAAACCCTGGAGCCTTGTCGTGATCCCTGAGGCCGATG580.37973026057352366No Hit
GTCGTGACTGGGAAAACCCTGGCATTATGTCGTGATTCTGACTACCGAAG570.3731831871153594No Hit
GTCGTGACTGGGAAAACCCTGGCTGTCTGTCGTGATCATAAGGAACGAAT540.35354196674086685No Hit
GTCGTGACTGGGAAAACCCTGGCACACAGTCGTGATCCGCTAATCCCATT520.3404478198245384No Hit
GTCGTGACTGGGAAAACCCTGGATTGACGTCGTGATCTACAACATCGATG510.33390074636637423No Hit
GTCGTGACTGGGAAAACCCTGGTGGACAGTCGTGATACCCGACCCCGAAT510.33390074636637423No Hit
GTCGTGACTGGGAAAACCCTGGATGGCCGCGTGACTGCTTAAAGGCGAAT490.32080659945004586No Hit
GTCGTGACTGGGAAAACCCTGGGACGACGTCGTGATAACCTTTGGCGATG480.3142595259918816No Hit
GTCGTGACTGGGAAAACCCTGGTTATAAGTCGTGATTGCGGGAACCGAAT420.27497708524289644No Hit
GTCGTGACTGGGAAAACCCTGGTCAGCCGTCGTGATCTACAGAAACATGG400.261882938326568No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTACATCGGGGGGGAGCGATG380.2487887914102396No Hit
GTCGTGACTGGGAAAACCCTGGTACTACGTCGTGATGAAGTCGCTCATGG380.2487887914102396No Hit
GTCGTGACTGGGAAAACCCTGGTTTTCTGTCGTGATCGAGGCGGTCGAAT330.2160534241194186No Hit
GTCGTGACTGGGAAAACCCTGGCACCGCGTCGTGATTTAGTAGCTCAATG330.2160534241194186No Hit
GTCGTGACTGGGAAAACCCTGGCCAGACGTCGTGATTTTAATCCTCGAAT320.20950635066125442No Hit
GTCGTGACTGGGAAAACCCTGGTGTTTGGTCGTGATCCTGCCCTACGACG320.20950635066125442No Hit
GTCGTGACTGGGAAAACCCTGGGCTGCGGTCGTGATATGAGGGCGCGAAT310.2029592772030902No Hit
GTCGTGACTGGGAAAACCCTGGAGGCACGTCGTGATACAATGGATCGATG300.19641220374492602No Hit
GTCGTGACTGGGAAAACCCTGGATATTTGTCGTGATTCCTTGGCCCGACG300.19641220374492602No Hit
GTCGTGACTGGGAAAACCCTGGCCCCACGTCGTGATCCAGCGGGTCGAAT290.18986513028676183No Hit
GTCGTGACTGGGAAAACCCTGGGCAAAAGTCGTGATTAGACGTTGCAATG280.18331805682859761No Hit
GTCGTGACTGGGAAAACCCTGGACTCCCGTCGTGATATCAAGGCTCAATG280.18331805682859761No Hit
GTCGTGACTGGGAAAACCCTGGCTATGCGTCGTGATCGTTACAAACAATG270.17677098337043343No Hit
GTCGTGACTGGGAAAACCCTGGTGTAGCGTCGTGATATTATGCAACGACG270.17677098337043343No Hit
GTCGTGACTGGGAAAACCCTGGTCGACGGTCGTGATCGCATCGGCCAATG270.17677098337043343No Hit
GTCGTGACTGGGAAAACCCTGGTCTACTGTCGTGATGCAAATGGTCAATG260.1702239099122692No Hit
GTCGTGACTGGGAAAACCCTGGAGGTTTGTCGTGATTTGTCTCCCCGATG260.1702239099122692No Hit
GTCGTGACTGGGAAAACCCTGGAAAATCGTCGTGATGGAGCTTGCCGAAT240.1571297629959408No Hit
GTCGTGACTGGGAAAACCCTGGATACAAGTCGTGATAATCACTGTGGAAG220.1440356160796124No Hit
GTCGTGACTGGGAAAACCCTGGTGCGCGGTCGTGATTGAGATTTCCGACG210.13748854262144822No Hit
GTCGTGACTGGGAAAACCCTGGCTCTCCGTCGTGATAATGTACCACGAAT210.13748854262144822No Hit
GTCGTGACTGGGAAAACCCTGGAAAGGTGTCGTGATACAGCGTGACAATG210.13748854262144822No Hit
GTCGTGACTGGGAAAACCCTGGAATTCGGTCGTGATGTTCGCAATCGACG210.13748854262144822No Hit
GTCGTGACTGGGAAAACCCTGGGGCGAGGTCGTGATGCCTTCAATCGACG200.130941469163284No Hit
GTCGTGACTGGGAAAACCCTGGCTGACCGTCGTGATACAAATATGCGAAT190.1243943957051198No Hit
GTCGTGACTGGGAAAACCCTGGCACCCCGTCGTGATGGAAGTAACCGATG180.11784732224695563No Hit
GTCGTGACTGGGAAAACCCTGGGACTAAGTCGTGATCTGGACAGGCGAAT180.11784732224695563No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGATG170.11130024878879141No Hit
GTCGTGACTGGGAAAACCCTGGTTGTGCGTCGTGATGCTGGCTAACAATG170.11130024878879141No Hit
GTCGTGACTGGGAAAACCCTGGATAACCGTCGTGATGTAATAGGACGACG170.11130024878879141No Hit
GTCGTGACTGGGAAAACCCTGGCCAAAGGTCGTGATAATGCGGTGCAATG160.10475317533062721No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTACATCGACCACAAGCGATG160.10475317533062721No Hit
GTCGTGACTGGGAAAACCCTGGGTGAATGTCGTGATTGGCAAGATCGATG160.10475317533062721No Hit
GTCGTGACTGGGAAAACCCTGGAAACGTGTCGTGATGGTCTATTTCCATG160.10475317533062721No Hit
GTCGTGACTGGGAAAACCCTGGTCAATTGTCGTGATCACCCGCCGCAATG160.10475317533062721No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGC151.5451027E-5242.78622
CGTGGCT301.6370905E-11242.78623
CGAGACT100.0015022182242.78623
TCGTGAC14050.0242.786182
AATCATA100.00151711241.990177
TGGGGAA100.00151711241.990179
ACTGGGA13800.0241.990167
TGACTGG14100.0241.132055
GTGACTG14100.0241.132054
TGGGAAA13550.0240.204279
GACTGGG14000.0239.397426
CGTGACT14400.0237.728153
CTGGGAA14300.0230.144498
GACTGGA304.9276423E-9201.658486
TGGGAGA254.8914444E-7193.592139
GGCGTGG204.8674414E-5182.089651
TCGTGGC204.8674414E-5182.089652
CTGGGGG351.238368E-8172.850118
GCTGGGG506.020855E-10145.19417
GTCGTGA25800.0131.744451
Produced by FastQC (version 0.11.5)