FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073413
Sequences flagged as poor quality0
Sequence length248
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGAGTCCTGTCGTGATCTAAGTTGGTGACC105390.9818215356065186No Hit
GTCGTGACTGGGAAAACCCTGGTCACGCGTCGTGATCCTTGTATCTGACC53530.49868969352895853No Hit
GTCGTGACTGGGAAAACCCTGGAGACTTGTCGTGATCAGTCCTTTTGACC38100.35494259898100733No Hit
GTCGTGACTGGGAAAACCCTGGCACAGGGTCGTGATATAGGACTCTGACC35570.3313729198360743No Hit
GTCGTGACTGGGAAAACCCTGGAAACACGTCGTGATGGTGACGGATGACC24770.23075926973122182No Hit
GTCGTGACTGGGAAAACCCTGGCCCAAAGTCGTGATCGAAACCGATGACC23250.21659883008683517No Hit
GTCGTGACTGGGAAAACCCTGGGATCTCGTCGTGATACTAGCGAGTGACC21950.20448792775939922No Hit
GTCGTGACTGGGAAAACCCTGGGAACTCGTCGTGATTATGATTCCTGACC15540.1447718632064266No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGACC14460.13471049819594136No Hit
GTCGTGACTGGGAAAACCCTGGCCGGAGGTCGTGATCGTGCGAGCTGACC13760.12818924309655277No Hit
GTCGTGACTGGGAAAACCCTGGTTCACGGTCGTGATTAGTACGGCTGACC13620.12688499207667506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA1050400.0240.763577
GTGACTG1056000.0240.355474
TGACTGG1055850.0240.333345
TCGTGAC1059400.0240.026542
TGGGAAA1053900.0239.791769
GACTGGG1068150.0237.124886
CGTGACT1075500.0236.221663
CTGGGAA1099750.0229.849568
GACTGCG1250.0135.517656
TGACCGG3800.0130.568625
TGCGAAA1300.0130.299369
ACCGGGA3350.0130.021557
GTCGTGA2052900.0123.770251
CTGCGAA1300.0120.992278
ACTGGAA5000.0113.732747
GACCGGG4550.0106.371796
CGGGGAA1950.0105.480449
TCGAGAC2350.0103.0729752
CTCGGAA1300.0102.3780758
GACTCGG1350.098.590886
Produced by FastQC (version 0.11.5)