FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169700
Sequences flagged as poor quality0
Sequence length248
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCGAAAAGTCGTGATGACGCATGCATCAC301242.5753612037274514No Hit
GTCGTGACTGGGAAAACCCTGGGCATAGGTCGTGATTGTTTACCCATCAC222881.905445840813884No Hit
GTCGTGACTGGGAAAACCCTGGGACAAGGTCGTGATTACGAGAGCATCAC220901.8885184235274002No Hit
GTCGTGACTGGGAAAACCCTGGACCGGTGTCGTGATTCGAACCCGATCAC192991.6499102333931777No Hit
GTCGTGACTGGGAAAACCCTGGCCGCAAGTCGTGATACCATGAGCATCAC182141.557151406343507No Hit
GTCGTGACTGGGAAAACCCTGGAATGTCGTCGTGATGTGAACTCGATCAC178861.5291100282123622No Hit
GTCGTGACTGGGAAAACCCTGGATCCTTGTCGTGATACAAACCCGATCAC172071.4710609558006327No Hit
GTCGTGACTGGGAAAACCCTGGTCAGCCGTCGTGATGGACAGTAGATCAC147831.2638283320509531No Hit
GTCGTGACTGGGAAAACCCTGGGCTGTTGTCGTGATAAATGCGATATCAC147381.2599811917585706No Hit
GTCGTGACTGGGAAAACCCTGGATTACAGTCGTGATGGCTGGTCGATCAC138121.180815593741985No Hit
GTCGTGACTGGGAAAACCCTGGGCAAGCGTCGTGATATGTTGAGTATCAC135461.1580747200136787No Hit
GTCGTGACTGGGAAAACCCTGGAAACATGTCGTGATCTTACGTGGATCAC131551.1246473454731982No Hit
GTCGTGACTGGGAAAACCCTGGTCATACGTCGTGATTTGTGCAAAATCAC126811.0841241343934342No Hit
GTCGTGACTGGGAAAACCCTGGCACAGGGTCGTGATCCTGATAGCATCAC108240.9253654783277764No Hit
GTCGTGACTGGGAAAACCCTGGTCTGCAGTCGTGATCTTCACCGAATCAC107020.9149354535350944No Hit
GTCGTGACTGGGAAAACCCTGGCTACGCGTAGTGATCGCAAGAAGATCAC105220.8995468923655637No Hit
GTCGTGACTGGGAAAACCCTGGCATGTAGTCGTGATTTCCTGTATATCAC101200.865179105753612No Hit
GTCGTGACTGGGAAAACCCTGGCTACTGGTCGTGATTCCAATATTATCAC97910.8370522356159699No Hit
GTCGTGACTGGGAAAACCCTGGACTCGCGTCGTGATTGATACAAGATCAC89290.7633581260152176No Hit
GTCGTGACTGGGAAAACCCTGGAGCGTAGTCGTGATTCCGCGTCCATCAC89280.7632726340087201No Hit
GTCGTGACTGGGAAAACCCTGGAAGCAGGTCGTGATAAAAGATTTATCAC89060.7613918098657776No Hit
GTCGTGACTGGGAAAACCCTGGTAGACTGTCGTGATAAATATAGCATCAC88410.755834829443447No Hit
GTCGTGACTGGGAAAACCCTGGGTTCTCGTCGTGATTGGCCGTTTATCAC79290.6778661195178252No Hit
GTCGTGACTGGGAAAACCCTGGGTTGCAGTCGTGATGTACTGGATATCAC71060.607506198170471No Hit
GTCGTGACTGGGAAAACCCTGGCGGCGCGTCGTGATTCAAATAGCATCAC70540.6030606138326067No Hit
GTCGTGACTGGGAAAACCCTGGTCTATGGTCGTGATTGCGATGATATCAC69790.5966487133453022No Hit
GTCGTGACTGGGAAAACCCTGGTATGCTGTCGTGATCGTAAAATAATCAC67440.576558091818415No Hit
GTCGTGACTGGGAAAACCCTGGCCGGCTGTCGTGATGATATACCGATCAC67100.5736513635975036No Hit
GTCGTGACTGGGAAAACCCTGGTCCAAGTCCTGATAGTAACAGCGATCAC66680.5700606993246131No Hit
GTCGTGACTGGGAAAACCCTGGTGATACGTCGTGATAACATTCCGATCAC62190.5316747884072839No Hit
GTCGTGACTGGGAAAACCCTGGACAAAGGTCGTGATATGAAGAAAATCAC62160.5314183123877918No Hit
GTCGTGACTGGGAAAACCCTGGAGGCAGGTCGTGATTTGGAGGAGATCAC61850.5287680601863726No Hit
GTCGTGACTGGGAAAACCCTGGTAGACTGTCGTGATCAATGCGAGATCAC61100.5223561596990681No Hit
GTCGTGACTGGGAAAACCCTGGACGTTCGTCGTGATAACTGCCCGATCAC60940.5209882875951098No Hit
GTCGTGACTGGGAAAACCCTGGGGTCAAGTCGTGATTGAGGCAAAATCAC58060.4963665897238608No Hit
GTCGTGACTGGGAAAACCCTGGAAAACCGTCGTGATCGGAATCCTATCAC52920.45242369838420105No Hit
GTCGTGACTGGGAAAACCCTGGAGGATCGTCGTGATTATAGTCCCATCAC51420.4395998974095922No Hit
GTCGTGACTGGGAAAACCCTGGTACAGTGTCGTGATGCACTCCCCATCAC49870.4263486364024963No Hit
GTCGTGACTGGGAAAACCCTGGTGGAGTGTCGTGATCTATCCAAAATCAC49060.41942378387620755No Hit
GTCGTGACTGGGAAAACCCTGGTGCCCCGTCGTGATGTAAGCAGTATCAC44380.3794135248354279No Hit
GTCGTGACTGGGAAAACCCTGGGACCTGGTCGTGATATGCAGAACATCAC43970.37590835256903476No Hit
GTCGTGACTGGGAAAACCCTGGCAGTGAGTCGTGATTGCCTACCCATCAC42750.3654783277763529No Hit
GTCGTGACTGGGAAAACCCTGGCGTTACGTCGTGATTATGCACCGATCAC42390.3624006155424468No Hit
GTCGTGACTGGGAAAACCCTGGGATCGGGTCGTGATGAACTCCAGAGCAC41910.35829699923057196No Hit
GTCGTGACTGGGAAAACCCTGGTCCAAGTCGTGATAGTAACAGCGATCAC41500.3547918269641789No Hit
GTCGTGACTGGGAAAACCCTGGGACCCGGTCGTGATATATGCTCCATCAC39900.34111310592459604No Hit
GTCGTGACTGGGAAAACCCTGGGCCATGGTCGTGATAACAATGCCATCAC37000.3163204240403522No Hit
GTCGTGACTGGGAAAACCCTGGACCTGTGTCGTGATTGGATGTGGATCAC36160.30913909549457125No Hit
GTCGTGACTGGGAAAACCCTGGAGATCAGTCGTGATTAGGATTCAATCAC35150.3005044028383346No Hit
GTCGTGACTGGGAAAACCCTGGCCGTCAGTCGTGATCACCGATTAATCAC33450.2859707617337779No Hit
GTCGTGACTGGGAAAACCCTGGGCTGTTGTCGTGATAAATGCGATACACG33430.28579977772078313No Hit
GTCGTGACTGGGAAAACCCTGGATCATCGTCGTGATACCTAGAACATCAC32760.2800718132854578No Hit
GTCGTGACTGGGAAAACCCGGGGCATAGGTCGTGATTGTTTACCCATCAC31030.26528169616140895No Hit
GTCGTGACTGGGAAAACCCTGGTGCGTGGTCGTGATCCTGCCGTCATCAC30210.25827135162862275No Hit
GTCGTGACTGGGAAAACCCTGGACTTTGTCGTGATTCCAACGACCATCAC29470.2519449431478157No Hit
GTCGTGACTGGGAAAACCCTGGCGTGGTGTCGTGACTAGATAGGCATCAC28540.2439941865435582No Hit
GTCGTGACTGGGAAAACCCTGGCGTTACGTCGTGATGGAGGCGTCATCAC26550.22698127725057707No Hit
GTCGTGACTGGGAAAACCCGGGGTTCTCGTCGTGATTGGCCGTTTATCAC25760.22022740873728305No Hit
GTCGTGACTGGGAAAACCCTGGTAGCGGGTCGTGATAACTTCACCATCAC25120.21475592032144994No Hit
GTCGTGACTGGGAAAACCCTGGCCACTCGTCGTGATGCAAGCGTCATCAC25010.2138155082499786No Hit
GTCGTGACTGGGAAAACCCTGGGTCCAGGTCGTGATAAAGGCCAGATCAC23630.20201761135333848No Hit
GTCGTGACTGGGAAAACCCTGGCACTCCGTCGTGATAAAACTTCCATCAC23620.20193211934684108No Hit
GTCGTGACTGGGAAAACCCTGGTAGCATGTCGTGATTGATCCAAAATCAC23560.2014191673078567No Hit
GTCGTGACTGGGAAAACCCTGGACTTTGTCCTGATTCCAACGACCATCAC23530.20116269128836453No Hit
GTCGTGACTGGGAAAACCCTGGCATGCTGTCGTGATCGTATGTGTATCAC22500.1923570146191331No Hit
GTCGTGACTGGGAAAACCCTGGACTCCCGTCGTGATTGCGGACAGATCAC22200.18979225442421135No Hit
GTCGTGACTGGGAAAACCCTGGGGTGGTGTCGTGATTCGAACCCGATCAC21670.18526117807984954No Hit
GTCGTGACTGGGAAAACCCTGGTACCCTGTCGTGATATAAAGGGCATCAC20560.17577156535863897No Hit
GTCGTGACTGGGAAAACCCTGGAGTCTTGTCGTGATCATTGCGCTATCAC20500.17525861331965462No Hit
GTCGTGACTGGGAAAACCCTGGGCGTAAGTCGTGATTGGCGTGCCATCAC20070.17158245704026673No Hit
GTCGTGACTGGGAAAACCCTGGCAGGGCGTCGTGATAAGTACGGCATCAC19880.1699581089168163No Hit
GTCGTGACTGGGAAAACCCTGGTGGTTTGTCGTGATCTGGGAGGAATCAC19870.16987261691031888No Hit
GTCGTGCCTGGGAAAACCCTGGCTACTGGTCGTGATTCCAATATTATCAC19440.166196460630931No Hit
GTCGTGACTGGGAAAACCCTGGCGCGGGTCGTGATAAGTACGGCAATCAC19130.16354620842951184No Hit
GTCGTGACTGGGAAAACCCTGGACGATCGTCGTGATTGGCCGTTTATCAC19080.16311874839702487No Hit
GTCGTGACTGGGAAAACCCTGGCAATTGGTCGTGAGGTAACCGCGATCAC19060.1629477643840301No Hit
GTCGTGACTGGGAAAACCCGGGCTACTGGTCGTGATTCCAATATTATCAC18700.15987005215012395No Hit
GTCGTGACTGGGAAAACCCTGGTGCCAAGTCGTGATTTGGCGACTATCAC17490.14952551936393946No Hit
GTCGTGACTGGAAAACCCTGGGCTGTTGTCCTGATAAATGCGATAATCAC17270.14764469522099685No Hit
GTCGTGACTGGGAAAACCCTGGAGCTTGTCCTGATTCACTGAAAAATCAC17030.1455928870650594No Hit
GTCGTGACTGGGAAAACCCTGGCGCGGGTCCTGATAAGTACGGCAATCAC16330.13960844661024194No Hit
GTCGTGACTGGGAAAACCCTGGCGCTGTGTCGTGATGACCCGTCGATCAC16140.1379840984867915No Hit
GTCGTGACTGGGAAAACCCTGGCATATCGTCGTGATAGGCGATGGATCAC16020.13695819440882276No Hit
GTCGTGACTGGGAAAACCCTGGGGATTCGTCGTGATGCTACCCACATCAC15730.1344789262203984No Hit
GTCGTGACTGGGAAAACCCGGGATTACAGTCGTGATGGCTGGTCGATCAC15610.13345302214242968No Hit
GTCGTGACTGGGAAAACCCTGGCGTCCGTCCTGATCCGAGGTTCCCATCA15550.13294007010344533No Hit
GTCGTGACTGGGAAAACCCTGGACCGGAGTCGTGATCTAAGAAGCATCAC15350.13123022997349748No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGTGTCGTGATAGCACGACCATCAC15290.13071727793451313No Hit
GTCGTGACTGGGAAAACCCGGGAATGTCGTCGTGATGTGAACTCGATCAC15010.12832350175258614No Hit
GTCGTGACTGGGAAAACCCTGGCACTCAGTCGTGATTGCCATAGGATCAC14660.1253312815251774No Hit
GTCGTGACTGGGAAAACCCTGGAAGCAGGTCGTGATAAAAGATTTTCACG14280.12208258527827648No Hit
GTCGTGACTGGGAAAACCCTGGCAGCAAGTCGTGATATAGGGATTATCAC13880.11866290501838078No Hit
GTCGTGACTGGGAAAACCCTGGGACCTGGTCGAACCCTGGCCCTGATCAC13860.118491921005386No Hit
GTCGTGACTGGGAAAACCCTGGCTAAGTGTCGTGATGAGCTTCCCACCTC13660.11678208087543814No Hit
GTCGTGACTGGGAAAACCCGGGCGAAAAGTCGTGATGACGCATGCATCAC13660.11678208087543814No Hit
GTCGTGACTGGGAAAACCCTGGCCCGCTGTCGTGATCCCACCGTCATCAC13520.11558519278447466No Hit
GTCGTGACTGGGAAAACCTGGCGTGACGTCGTGATACAAATGGGCATCAC13220.11302043258955288No Hit
GTCGTGACTGGAAAACCCTGGGCTGTTGTCGTGATAAATGCGATAATCAC13150.11242198854407112No Hit
GTCGCGACTGGGAAAACCCTGGGCCATGGTCGTGATAACAATGCCATCAC12920.1104556723946311No Hit
GTCGTGACTGGGAAAACCCGGGATCCTTGTCGTGATACAAACCCGATCAC12830.10968624433615456No Hit
GTCGTGACTGGGAAAACCCTGGGATCTCGTCGTGATCGATAGTAGAGCAC12610.10780542019321195No Hit
GTCGTGACTGGGAAAACCCGGGCATGTAGTCGTGATTTCCTGTATATCAC12390.10592459605026928No Hit
GTCGTGACTGGGAAAACCCGGGTCAGCCGTCGTGATGGACAGTAGATCAC12300.10515516799179277No Hit
GTCGTGACTGGGAAAACCCGGGTCTGCAGTCGTGATCTTCACCGAATCAC12230.10455672394631102No Hit
GTCGTGACTGGGAAAACCCTGGAATAACGTCTGATTGACACATCGATCAC12210.10438573993331622No Hit
GTCGTGACTGGGAAAACCCTGGACTCGTGTCGTGATACTTAATGGATCAC12060.10310335983585535No Hit
GTCGTGACTGGGAAAACCCTGGTACATCGTCGTGATACACCGGCCATCAC11760.10053859964093358No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG1050600.0241.062454
TGACTGG1050600.0240.94665
TCGTGAC1019500.0240.879152
ACTGGGA1059000.0240.867977
GACTGGG1068050.0240.663096
GGTGACT54100.0239.184723
TGGGAAA1038150.0238.79249
CTGGGAA1070450.0237.727268
TGGTGAC65750.0236.411152
CGTGACT1023450.0234.838273
TGAGTGG11550.0232.699775
GTGGTGA72500.0231.046161
CGTGAGT6900.0230.028523
GTGAGTG11750.0228.827014
GGGACTG20800.0225.923074
TGACGGG34250.0223.399125
TCGGGAC16150.0222.976752
GGACTGG21250.0222.193135
CGGGGAA40000.0221.211698
GTGACGG35250.0217.832324
Produced by FastQC (version 0.11.5)