FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1642045
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTGCTCTGTCGTGATCTCCCGCCCACTTG20800.12667131534154058No Hit
GTCGTGACTGGGAAAACCCTGGGCTTGTGTCGTGATGCGCCAGCCACTTG19910.12125124463702275No Hit
GTCGTGACTGGGAAAACCCTGGGACGGCGTCGTGATAGTACGAGCACTTG18880.11497857854078299No Hit
GTCGTGACTGGGAAAACCCTGGCTCGTTGTCGTGATTAGCGCCCCACTTG18770.11430868216157292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC1517100.0241.41112
TGACTGG1532400.0241.133075
GTGACTG1533300.0240.996114
GACTGGG1557050.0239.830676
CGTGACT1506000.0239.572333
ACTGGGA1555650.0239.223927
TGGGAAA1552900.0234.862789
CTGGGAA1589550.0233.179678
CGTGACG17450.0211.099883
CGTGACA20400.0206.114793
GACGGGG32350.0205.109186
TGACGGG28050.0205.077965
ACGGGGA30050.0201.436517
GTGACGG31350.0182.365264
GGTGACT51150.0175.305663
GCGTGAC13700.0166.368972
GGCGTGA14600.0165.368761
AGGGAAA27750.0164.456599
GTGGTGA70650.0163.656781
GTGAGTG11700.0160.466194
Produced by FastQC (version 0.11.5)