FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069402
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGATCA13660.12773493971397099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT1048600.0241.607593
TGACTGG1049900.0241.20615
GTGACTG1050300.0241.113754
TCGTGAC1052550.0240.871082
GACTGGG1053650.0240.049736
ACTGGGA1055100.0239.605947
CTGGGAA1074800.0235.101628
TGGGAAA1095800.0230.62959
ACGTGAC1700.0192.376622
CGAGACT2800.0142.688863
CGGGGAA2150.0135.065029
GTCGTGA2043400.0123.952371
TCGTGGC3600.0121.1260152
ACCGGGA3150.0119.080757
CGTGACC3200.0117.285933
TCGAGAC3250.0111.8086242
CGTGGCT4750.0104.501963
GACGTGA2850.0102.043841
TCGGAAA1500.088.730229
CTCGTGA3150.088.4784851
Produced by FastQC (version 0.11.5)