FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194154
Sequences flagged as poor quality0
Sequence length248
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCAGAT49830.41728286301431805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT1162350.0241.341843
GTGACTG1163550.0241.158134
TGACTGG1164100.0240.98515
TCGTGAC1165850.0240.891942
ACTGGGA1164100.0240.569347
GACTGGG1165800.0240.322346
TGGGAAA1195300.0234.17869
CTGGGAA1219450.0229.630238
ACGTGAC2550.0171.105412
ACCGGGA2700.0152.366177
CGGGGAA2350.0144.166249
CGAGACT2550.0137.719393
GACGTGA3200.0132.595491
TCGAGAC2600.0130.52272
TCGTGGC2800.0125.528212
TGCGAAA1950.0124.099159
GTCGTGA2262700.0124.032281
TGACCGG4050.0122.4904565
CGTTACT1000.0108.988273
GCGTGGC2450.0108.832352
Produced by FastQC (version 0.11.5)