FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317826
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTGCTTAGTCGTGATTTACGGAAAGCCAA43801.3781125521511772No Hit
GTCGTGACTGGGAAAACCCTGGATATTAGTCGTGATTATAACCGCACCAA4320.135923429801212No Hit
GTCGTGACTGGGAAAACCCTGGACGCATGTCGTGATATATTTCACACCAA4210.13246241654238483No Hit
GTCGTGACTGGGAAAACCCTGGGAAATTGTCGTGATCGTTACCGTGCCAA3880.12207937676590336No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAC100.0015261461242.264632
TGACTGG293050.0241.575075
GTGACTG293350.0241.53114
CGTGACT289800.0241.519783
TCGTGAC296650.0240.794632
GACTGGG296250.0240.235436
ACTGGGA294200.0239.89357
TGGGAAA291200.0238.000639
CGTGGCT3550.0235.366153
CTGGGAA300100.0234.249538
GCGTGAC1600.0211.981552
TGACGGG2800.0211.848085
TCGTGGC3900.0201.887192
CGTGACA4500.0199.132643
GTGGTGA6150.0193.115131
TGACAGG4550.0191.561225
AGGGAAA4750.0191.111059
ACGGGGA3050.0190.484447
GTGACGG3200.0189.179844
CGTGACG2800.0185.966053
Produced by FastQC (version 0.11.5)