FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4541522
Sequences flagged as poor quality0
Sequence length248
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTAGCT58260.1282829853075687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT4467650.0241.577623
TGACTGG4468050.0241.558655
GTGACTG4470300.0241.378724
TCGTGAC4479050.0241.309342
ACTGGGA4466900.0241.18517
GACTGGG4469350.0241.174476
CTGGGAA4639600.0232.168418
TGGGAAA4675350.0230.406139
TGACCGG8100.0222.597785
ACGTGAC5200.0214.336882
ACCGGGA7950.0193.293817
TCGTGGC9450.0169.22112
CGTGGCT9450.0166.5693
CGGGGAA7400.0161.873329
CGTGACC10150.0161.046173
TGACGGG5600.0142.618385
ACTGGAA14850.0136.072817
CCGTGAC3050.0127.104982
GTCGTGA8770500.0123.164351
GACGGGA6050.0112.005136
Produced by FastQC (version 0.11.5)