FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635232
Sequences flagged as poor quality0
Sequence length248
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGACACACGTCGTGATATGAACCCCCAGTT12540.1974081910231223No Hit
GTCGTGACTGGGAAAACCCTGGCCCTTGGTCGTGATGAAAACGCGCAGTT8060.126882776686313No Hit
GTCGTGACTGGGAAAACCCTGGCGGCTCGTCGTGATCGTCTGTGGCAGAT6650.1046861619062012No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC579200.0241.787582
CGTGACT571150.0241.500273
TGACTGG580700.0241.276085
GTGACTG581300.0241.082174
ACTGGGA584650.0239.711737
GACTGGG588250.0239.479586
TGGGAAA582000.0237.518629
CTGGGAA597800.0233.834848
CGTGACA7900.0210.101043
TGACGGG7500.0208.268525
CGTGACG4900.0205.218633
GGTGACT11050.0205.028433
GTGGTGA15000.0202.953611
TAGATCG2150.0202.717297
AAGATCG4800.0196.734317
GACGGGG8500.0195.131966
ACGGGGA7550.0192.424797
TCGTGGC3250.0186.52182
GTGACGG8450.0186.345414
GAGATCG3250.0182.53227
Produced by FastQC (version 0.11.5)