FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794123
Sequences flagged as poor quality0
Sequence length248
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTAGG10000.12592507709762846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA779700.0241.03087
TCGTGAC781750.0240.875152
GTGACTG782400.0240.661714
TGACTGG784350.0240.002645
TGGGAAA783800.0239.56939
GACTGGG790800.0237.72416
CGTGACT804850.0233.903123
CTGGGAA813400.0230.970298
ACGTGAC1300.0214.219072
CTGCGAA1200.0181.49478
GACTGGA2950.0151.75836
TCGAGAC1800.0141.260392
TGCGAAA1550.0140.512029
GCGTGGC1350.0134.53372
TCGCGAC900.0134.53372
GTCGTGA1512650.0124.3973851
GCGACTG1000.0121.026964
GTCGCGA1050.0115.350951
CGAGACT2350.0113.316113
CGGGGAA1950.0111.689069
Produced by FastQC (version 0.11.5)