FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647202
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGACCTTAGTCGTGATCTTTACTTGTGACC218901.328920193151781No Hit
GTCGTGACTGGGAAAACCCTGGTCACATGTCGTGATGACAATCCCTGACC179941.092397896554278No Hit
GTCGTGACTGGGAAAACCCTGGTGAGATGTCGTGATAGCCCGTGGTGACC168861.0251323152837357No Hit
GTCGTGACTGGGAAAACCCTGGGTTCGCGTCGTGATTAAGTATGCTGACC165621.005462596572855No Hit
GTCGTGACTGGGAAAACCCTGGCCCTACGTCGTGATCTAACGAGCTGACC157690.9573203529378911No Hit
GTCGTGACTGGGAAAACCCTGGGAGTCTGTCGTGATACGTATCCGTGACC154300.9367399991015067No Hit
GTCGTGACTGGGAAAACCCTGGATGTGCGTCGTGATATTGGCTCCTGACC145850.8854408870314631No Hit
GTCGTGACTGGGAAAACCCTGGTCGTGTGTCGTGATGCCAGGTAGTGACC145770.8849552149645277No Hit
GTCGTGACTGGGAAAACCCTGGTTACCAGTCGTGATCAGTGCACTTGACC138570.8412447289403485No Hit
GTCGTGACTGGGAAAACCCTGGGGTCAGGTCGTGATTCGAAGTATTGACC138390.840151966789744No Hit
GTCGTGACTGGGAAAACCCTGGCAGGCTGTCGTGATGTAGTATCCTGACC137670.8357809181873262No Hit
GTCGTGACTGGGAAAACCCTGGATACCAGTCGTGATAAAGACCCGTGACC137080.832199086693678No Hit
GTCGTGACTGGGAAAACCCTGGAATACCGTCGTGATAATAATTACTGACC134170.8145327652589056No Hit
GTCGTGACTGGGAAAACCCTGGGTACCGGTCGTGATGCGATACCGTGACC134090.8140470931919703No Hit
GTCGTGACTGGGAAAACCCTGGGACGGGGTCGTGATAGAAACAGCTGACC128560.780475011565066No Hit
GTCGTGACTGGGAAAACCCTGGATAATAGTCGTGATAAAAATCCGTGACC128530.7802928845399654No Hit
GTCGTGACTGGGAAAACCCTGGGCATGTGTCGTGATGGGGGTGCGTGACC123170.7477528560552986No Hit
GTCGTGACTGGGAAAACCCTGGATTCGTGTCGTGATCTTAACCGTTGACC123130.747510020021831No Hit
GTCGTGACTGGGAAAACCCTGGAAAATGGTCGTGATTTGAGGTGGTGACC122040.7408927381098371No Hit
GTCGTGACTGGGAAAACCCTGGATTCCTGTCGTGATCTTGAGTGGTGACC120270.730147243628893No Hit
GTCGTGACTGGGAAAACCCTGGCATGGCGTCGTGATGTGAAGGCGTGACC120000.7285081004029864No Hit
GTCGTGACTGGGAAAACCCTGGAGCCCAGTCGTGATTGGCAGTATTGACC119590.7260190310599429No Hit
GTCGTGACTGGGAAAACCCTGGTAGCACGTCGTGATGCAAGTGGGTGACC112080.6804265657763893No Hit
GTCGTGACTGGGAAAACCCTGGCGAGCCGTCGTGATACATTCCATTGACC111960.6796980576759863No Hit
GTCGTGACTGGGAAAACCCTGGATACGAGTCGTGATCTGCATTGCTGACC109570.6651886046762935No Hit
GTCGTGACTGGGAAAACCCTGGTATCAGGTCGTGATCAGTATTAGTGACC106380.6458224310072475No Hit
GTCGTGACTGGGAAAACCCTGGTATGTAGTCGTGATGCCGAGGGGTGACC106010.6435761976976716No Hit
GTCGTGACTGGGAAAACCCTGGCTCATTGTCGTGATTTGCACCGTTGACC105680.6415728004215634No Hit
GTCGTGACTGGGAAAACCCTGGCCTCAAGTCGATTGCACACCGTGTGACC104680.6355018995848718No Hit
GTCGTGACTGGGAAAACCCTGGAAAGTTGTCGTGTATGAAGTTGGTGACC98150.5958589171212759No Hit
GTCGTGACTGGGAAAACCCTGGACCTAGGTCGTGATAACAAGCCGTGACC97700.5931270117447648No Hit
GTCGTGACTGGGAAAACCCTGGGAAAGTGTCGTGATTGCAACCCGTGACC92540.5618011634274364No Hit
GTCGTGACTGGGAAAACCCTGGTCCAAAGTCGTGATTCAGTGTGCTGACC91870.557733659866853No Hit
GTCGTGACTGGGAAAACCCTGGAATGTCGTCGTGATTTGTATCCGTGACC91260.5540304103564712No Hit
GTCGTGACTGGGAAAACCCTGGATGCAAGTCGTGATGTGCTCTCCTGACC89620.5440741329842971No Hit
GTCGTGACTGGGAAAACCCTGGTCCCATGTCGTGATCACCTAGCCTGACC88510.5373354330555694No Hit
GTCGTGACTGGGAAAACCCTGGACAAACGTCGTGATAGCTTGGGTTGACC87620.5319323313109139No Hit
GTCGTGACTGGGAAAACCCTGGTATTCCGTCGTGATGGCACAGGGTGACC87460.5309609871770432No Hit
GTCGTGACTGGGAAAACCCTGGCTTCGAGTCGTGATTGCATAGATTGACC87340.5302324790766403No Hit
GTCGTGACTGGGAAAACCCTGGAGACCTGTCGTGATGTGAACGGGTGACC86150.5230081070809773No Hit
GTCGTGACTGGGAAAACCCTGGTGATGGGTCGTGATTCAGACCCGTGACC85900.5214903818718044No Hit
GTCGTGACTGGGAAAACCCTGGAATCCCGTCGTGATGGGTGGGGGTGACC85720.5203976197211999No Hit
GTCGTGACTGGGAAAACCCTGGGCAGAGGTCGTGATTAGGGTCCGTGACC84420.5125054486335009No Hit
GTCGTGACTGGGAAAACCCTGGATCCCCGTCGTGATCGCAACGGGTGACC84000.5099556702820904No Hit
GTCGTGACTGGGAAAACCCTGGGGACGAGTCGTGATTCAAACCGTTGACC82390.500181519935017No Hit
GTCGTGACTGGGAAAACCCTGGACAAACGTCGTGATGTATTGCCTTGACC81890.4971460695166713No Hit
GTCGTGACTGGGAAAACCCTGGACGTACGTCGTGATCACTAGCCATGACC81310.49362494703139015No Hit
GTCGTGACTGGGAAAACCCTGGCTTTCGGTCGTGATTAGCATCCGTGACC81120.4924714758724188No Hit
GTCGTGACTGGGAAAACCCTGGACCCCTGTCGTGATGCAAACCCGTGACC79380.48190810841657555No Hit
GTCGTGACTGGGAAAACCCCGTGGCGGCGACGACCCATTCGAACGTGACC78720.4779013138643591No Hit
GTCGTGACTGGGAAAACCCTGGAGCCTAGTCGTGATGTAATACCGTGACC78710.47784060485599217No Hit
GTCGTGACTGGGAAAACCCTGGTAAATTGTCGTGATTGTTAGTCCTGACC77970.47334813823684035No Hit
GTCGTGACTGGGAAAACCCTGGCTTCTCGTCGTGATATAAATCCGTGACC77210.46873425360095483No Hit
GTCGTGACTGGGAAAACCCTGGCCAACTGTCGTGATCCCCAGGGCTGACC68600.4164637973970406No Hit
GTCGTGACTGGGAAAACCCTGGCATATAGTCGTGATCCGGTTTCCTGACC68050.4131248019368602No Hit
GTCGTGACTGGGAAAACCCTGGGCAAGGGGCGGCGACGACCCATTTGACC67510.40984651548504675No Hit
GTCGTGACTGGGAAAACCCTGGGCGAGCGTCGTGATAAAAACAGTTGACC65490.39758329579492985No Hit
GTCGTGACTGGGAAAACCCTGGTCCGTAGTCGTGATGGACAGTTATGACC63190.3836202238705393No Hit
GTCGTGACTGGGAAAACCCTGGCGCGCCGTCGTGATACTAAATGGTGACC63170.3834988058538054No Hit
GTCGTGACTGGGAAAACCCTGGTTCCTTGTCGTGATGGGTGTTCCTGACC62710.3807061914689273No Hit
GTCGTGACTGGGAAAACCCTGGGCGGCAAGTCGTGATGACTGGGGTGACC60030.36443617722659394No Hit
GTCGTGACTGGGAAAACCCTGGCGATTCGTCGTGATTCTAATCCGTGACC58880.3574546412643987No Hit
GTCGTGACTGGGAAAACCCTGGCTGACCGTCGTGATAACTACCCGTGACC56680.34409865942367723No Hit
GTCGTGACTGGGAAAACCCTGGGCCACAGTCGTGATACCGCGCCCTGACC56470.342823770247972No Hit
GTCGTGACTGGGAAAACCCTGGTTCAAAGTCGTGATGCGGAGGCCTGACC53260.32333617856219216No Hit
GTCGTGACTGGGAAAACCCTGGGGCCCAGTCGTGATTTAAAGTGCTGACC52480.3186008759095727No Hit
GTCGTGACTGGGAAAACCCTGGAGCATAGTCGTGATGTCGTGTCCTGACC52280.31738669574223444No Hit
GTCGTGACTGGGAAAACCCTGGGTCCAAGTCGTGATAATCTAGGGTGACC52050.31599038854979533No Hit
GTCGTGACTGGGAAAACCCTGGGCGTACGTCGTATTACACCCCGTTGACC51890.3150190444159247No Hit
GTCGTGACTGGGAAAACCCTGGATCCCTGTCGTGATCCCCATGGGTGACC51580.3131370651565503No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGAGTCGTGATTAAGGCCCGTGACC51160.31058728680513986No Hit
GTCGTGACTGGGAAAACCCTGGTTCGCGGTCGTGATGCATGTGGGTGACC51120.31034445077167216No Hit
GTCGTGACTGGGAAAACCCTGGCTCGGGGTCGTGATGAAAATCCGTGACC50640.30743041837006024No Hit
GTCGTGACTGGGAAAACCCTGGACCAGTGTCGTGATGCCTACGGGTGACC50610.3072482913449595No Hit
GTCGTGACTGGGAAAACCCTGGTCCCCCGTCGTGATGCTGACCCTTGACC49980.3034236238178438No Hit
GTCGTGACTGGGAAAACCCTGGGAGGGGGTCGTGATGAGAACCCGTGACC49170.2985061941401237No Hit
GTCGTGACTGGGAAAACCCTGGTGTTTGTGTGTGTCTACCCCAACTGACC48270.29304238338710126No Hit
GTCGTGACTGGGAAAACCCTGGTATTAGGTCGTGATTCCTACCCTTGACC48090.29194962123649676No Hit
GTCGTGACTGGGAAAACCCTGGGCTAAGGTCGTGATTGAATTCCGTGACC47750.28988551495202164No Hit
GTCGTGACTGGGAAAACCCTGGATGCGAGTCGTGATGAAACGGGGTGACC46470.2821147618810565No Hit
GTCGTGACTGGGAAAACCCTGGGTTTGCAGTCGTGATAGTCGGTTTGACC45580.27671166013640097No Hit
GTCGTGACTGGGAAAACCCTGGTCCTCCGTCGTGATGCAAGTAAATGACC45060.2735547917013214No Hit
GTCGTGACTGGGAAAACCCTGGATGAGAGTCGTGATAAGGAATCGTGACC44030.2673017638395291No Hit
GTCGTGACTGGGAAAACCCTGGCCGTAAGTCGTGATACCATTTCCTGACC43940.26675538276422683No Hit
GTCGTGACTGGGAAAACCCTGGGAACGCGTCGTGATTCAATGGGGTGACC43780.2657840386303562No Hit
GTCGTGACTGGGAAAACCCTGGAGACCGGTCGTGATCCCTTCGCCTGACC43490.26402347738771564No Hit
GTCGTGACTGGGAAAACCCTGGGTCGCAGTCGTGATCAGCTACCGTGACC43380.2633556782956796No Hit
GTCGTGACTGGGAAAACCCTGGAATATTGTCGTGATAGGTACAACTGACC41930.25455287208247684No Hit
GTCGTGACTGGGAAAACCCTGGGCAATTGTCGTGATCTTGAATCCTGACC41000.24890693430435368No Hit
GTCGTGACTGGGAAAACCCGGGGGCGGGTCCTGATGGACAGGGGCTGACC40830.24787488116211612No Hit
GTCGTGACTGGGAAAACCCTGGCCACCTGTCGTGATATGGATTTGTGACC40210.24411092264336737No Hit
GTCGTGACTGGGAAAACCCTGGGTCCCCGTCGTGATACTGATCCGTGACC38130.23148344890304892No Hit
GTCGTGACTGGGAAAACCCGGGGGCGGGTCGTGATGGACAGGGGCTGACC37730.2290550885683723No Hit
GTCGTGACTGGGAAAACCCTGGTGTCTGGTCGTGATGATAAACCCTGACC37660.2286301255098039No Hit
GTCGTGACTGGGAAAACCCTGGAAATGTGTCGTGATGAGACGTGCTGACC37480.2275373633591994No Hit
GTCGTGACTGGGAAAACCCTGGTGTGAGGTCGTGATCGGAATACCTGACC36740.22304489674004768No Hit
GTCGTGACTGGGAAAACCCTGGCGAACCGTCGTGATGAAAACTGATGACC36720.22292347872331386No Hit
GTCGTGACTGGGAAAACCCTGGGTTCGGGTCGTGATTGGAACCGTTGACC36640.22243780665637852No Hit
GTCGTGACTGGGAAAACCCTGGGACCCGGTCGTGATACCTACCGTTGACC36030.21873455714599668No Hit
GTCGTGACTGGGAAAACCCTGGTTCGACGTCGTGATGGGGTAAAGTGACC35820.21745966797029145No Hit
GTCGTGACTGGGAAAACCCTGGATACGCGTCGTGATTAGATTTGCTGACC35180.21357429143480886No Hit
GTCGTGACTGGGAAAACCCTGGAGCGCTGTCGTGATGTCAACGCCTGACC35170.21351358242644192No Hit
GTCGTGACTGGGAAAACCCTGGCTATTCGTCGTGATAGATACGGGTGACC34220.20774622663158496No Hit
GTCGTGACTGGGAAAACCCTGGAATCCTGTCGTGATAGGTACAACTGACC34040.20665346448098046No Hit
GTCGTGACTGGGAAAACCCTGGGGCTGCGTCGTGATGCGATACCGTGACC33290.2021002888534618No Hit
GTCGTGACTGGGAAAACCCTGGCGCTTTGTCGTGATACGTTCCCCTGACC33160.2013110717446919No Hit
GTCGTGACTGGGAAAACCCTGGATTCCTGTCGTGATTCCCCGTCGTGACC32640.19815420330961228No Hit
GTCGTGACTGGGAAAACCCTGGGGTAGCGTCGTGATTAAGCCGGCTGACC29950.18182348005891202No Hit
GTCGTGACTGGGAAAACCCTGGTCGACTGTCGTGATTCTAGCCCCTGACC29120.17678463236445804No Hit
GTCGTGACTGGGAAAACCCTGGGGGGATGTCGTGATTTGATCTACTGACC28580.17350634591264458No Hit
GTCGTGACTGGGAAAACCCTGGTACACAGTCGTGATTGAAGCGACTGACC28190.17113869458633488No Hit
GTCGTGACTGGGAAAACCCTGGGACTTCGTCGTGATGCATGTGGGTGACC27650.16786040813452144No Hit
GTCGTGACTGGGAAAACCCTGGCTTCGAGTCGTGATAGTTGCGGGTGACC26860.16306439647353513No Hit
GTCGTGACTGGGAAAACCCTGGGCGCAAGTCGTGATGTGATTGATTGACC26760.16245730638986597No Hit
GTCGTGACTGGGAAAACCCTGGGCTATCGTCGTGAAATAACTAACTGACC26610.16154667126436223No Hit
GTCGTGACTGGGAAAACCCTGGCCTAAGGTCGTGATACGATAACTTGACC25970.15766129472887966No Hit
GTCGTGACTGGGAAAACCCTGGATGCAAGTCGTGATATACGCAGCTGACC25970.15766129472887966No Hit
GTCGTGACTGGGAAAACCCTGGCAAACCGTCGTGATAGGGCGGACTGACC24850.15086188579178508No Hit
GTCGTGACTGGGAAAACCCTGGCCTTTCGTCGTGATTGCTGTGGGTGACC24810.15061904975831744No Hit
GTCGTGACTGGGAAAACCCTGGGTGCATGTCGTGATAGTAAGCGTTGACC24470.1485549434738423No Hit
GTCGTGACTGGGAAAACCCTGGGCGGGTGTCGTGATATAACGTTGTGACC24400.1481299804152739No Hit
GTCGTGACTGGGAAAACCCTGGCTCTCGGTCGTGATACTGGCCCGTGACC24230.14709792727303633No Hit
GTCGTGACTGGGAAAACCCTGGCCTGACGTCGTGATTTAGATGATTGACC23940.14533736603039576No Hit
GTCGTGACTGGGAAAACCCTGGCGAAACGTCGTGATACTATAGGCTGACC23930.14527665702202885No Hit
GTCGTGACTGGGAAAACCCTGGTTCGTGGTCGTGATTAAAATAAATGACC23860.14485169396346045No Hit
GTCGTGACTGGGAAAACCCTGGGTGCTAGTCGTGATCTAGACCCGTGACC23760.1442446038797913No Hit
GTCGTGACTGGGAAAACCCTGGGGTAAAGTCGTGATATCGGGATCTGACC21920.13307414634027884No Hit
GTCGTGACTGGGAAAACCCTGGGGAGAGGTCGTGATGTAGTTTTTTGACC21910.13301343733191193No Hit
GTCGTGACTGGGAAAACCCTGGTCGTTGGTCGTGATACTGTCCCGTGACC21910.13301343733191193No Hit
GTCGTGACTGGGAAAACCCTGGTTGAAAGTCGTGATCGCATGTCATGACC21770.13216351121477513No Hit
GTCGTGACTGGGAAAACCCTGGGCCAATGTCGTGATGTCAATTTTTGACC20620.12518197525257985No Hit
GTCGTGACTGGGAAAACCCTGGCACTCGGTCGTGATCCGAATGAGTGACC20620.12518197525257985No Hit
GTCGTGACTGGGAAAACCCTGGGTTATAGTCGTGATGACGTGGGGTGACC20570.12487843021074525No Hit
GTCGTGACTGGGAAAACCCTGGAAAGCAGTCGTGATAGACTCACTTGACC20450.12414992211034227No Hit
GTCGTGACTGGGAAAACCCTGGCCTCAAGTCGATTTCACACCGTGTGACC20170.12245006987606863No Hit
GTCGTGACTGGGAAAACCCTGGTGTCGCGTCGTGATACAAATGAGTGACC20130.12220723384260097No Hit
GTCGTGACTGGGAAAACCCTGGCTTTAAGTCGTGATAGCAAATTCTGACC19720.11971816449955744No Hit
GTCGTGACTGGGAAAACCCTGGCAATATGTCGTGATACGTGTGGCTGACC19370.11759334920671538No Hit
GTCGTGACTGGGAAAACCCTGGAAACACGTCGTGATGACCTGAACTGACC19370.11759334920671538No Hit
GTCGTGACTGGGAAAACCCTGGCTAGGTGTCGTGATACCAGAACGTGACC19260.11692555011467932No Hit
GTCGTGACTGGGAAAACCCTGGAAAGAGGTCGTGATGAAGGTTAGTGACC19060.11571136994734102No Hit
GTCGTGACTGGGAAAACCCTGGCCATACGTCGTGATACCAGAACCTGACC18910.11480073482183727No Hit
GTCGTGACTGGGAAAACCCTGGCCGACAGTCGTGATATCGCGCCCTGACC18750.11382939068796663No Hit
GTCGTGACTGGGAAAACCCTGGCCCTCAGTCGTGATTAAGATAGTTGACC18610.1129794645708298No Hit
GTCGTGACTGGGAAAACCCGGGTGAGATGTCGTGATAGCCCGTGGTGACC18360.11146173936165693No Hit
GTCGTGACTGGGAAAACCCTGGCAAAGCGTCGTGATATGGAATGGTGACC18240.11073323126125394No Hit
GTCGTGACTGGGAAAACCCTGGAAGCTCGTCGTGATGAACACGGCTGACC18190.11042968621941934No Hit
GTCGTGACTGGGAAAACCCTGGCTCCGTGTCGTGATGCTAATGGGTGACC18090.10982259613575021No Hit
GTCGTGACTGGGAAAACCCTGGGAGGGGGTCGTGATTAGAGCTTTTGACC18070.10970117811901638No Hit
GTCGTGACTGGGAAAACCCTGGAAGGTTGTCGTGATCTACTTAGATGACC17990.10921550605208104No Hit
GTCGTGACTGGGAAAACCCTGGCTAAATGTCGTGATTTAAGGCCCTGACC17950.10897267001861337No Hit
GTCGTGACTGGGAAAACCCTGGACTACAGTCGTGATAGAGAGTGCTGACC17820.10818345290984348No Hit
GTCGTGACTGGGAAAACCCTGGCCATGCGTCGTGATTACAATCCTTGACC17540.10648360067556985No Hit
GTCGTGACTGGGAAAACCCTGGTGATCAGTCGTGATCAACTCCCGTGACC17150.10411594934926015No Hit
GTCGTGACTGGGAAAACCCGGGGAGTCTGTCGTGATACGTATCCGTGACC17000.1032053142237564No Hit
GTCGTGACTGGGAAAACCCTGGAAAGCCGTCGGTGCCAGTGCCGGTGACC16560.10053411785561212No Hit
GTCGTGACTGGGAAAACCCTGGGTCCTGGTCGTGATCTAGGATGTTGACC16510.10023057281377755No Hit
GTCGTGACTGGGAAAACCCTGGTTGACGGTCGTGATTCTAAGAACTGACC16510.10023057281377755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG1506250.0240.656434
GGTGACT73900.0240.300083
TGGGAAA1488100.0239.642939
TCGTGAC1466850.0239.288992
TGACTGG1517000.0238.931445
GACTGGG1546200.0238.259346
CGTGACT1448200.0238.201953
CGTGACA17100.0235.89163
GGGACTG26950.0234.568954
TGACGGG38700.0232.464945
ACGGGGA43650.0232.398797
ACTGGGA1580200.0232.253657
GTGACGG38800.0231.595954
GGTGACG15750.0231.499653
GTGACAG19350.0230.942664
CTGGGAA1587500.0230.021538
CGTGACG23500.0227.834123
TCGTGGC4950.0220.390142
ACTGGAA3950.0211.458957
AGGGAAA21550.0207.820779
Produced by FastQC (version 0.11.5)