FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559258
Sequences flagged as poor quality0
Sequence length248
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGATGTCGTCGTGATGAGATGGTTCGATG9210.16468249001355367No Hit
GTCGTGACTGGGAAAACCCTGGCCACTGGTCGTGATAAAGAGCCCCGATG7970.14251025465885156No Hit
GTCGTGACTGGGAAAACCCTGGGCCATGGTCGTGATACGGACACCCGATG7830.14000693776396583No Hit
GTCGTGACTGGGAAAACCCTGGCAGGTAGTCGTGATTCTAAGAAACGATG7430.13285460377857805No Hit
GTCGTGACTGGGAAAACCCTGGGCCGATGTCGTGATACATAGAACCGATG6900.12337776124793924No Hit
GTCGTGACTGGGAAAACCCTGGCTTCTAGTCGTGATGAGAGGTCCCGATG6730.12033801930414942No Hit
GTCGTGACTGGGAAAACCCTGGGTACTTGTCGTGATACCAAGATTCGATG6500.11622542726255146No Hit
GTCGTGACTGGGAAAACCCTGGAATAATGTCGTGATAACATGTCTCGATG6400.1144373437662045No Hit
GTCGTGACTGGGAAAACCCTGGCTATAAGTCGTGATATGAAGGATCGATG6190.11068236842387592No Hit
GTCGTGACTGGGAAAACCCTGGCACTTAGTCGTGATGTAGTCGGGCGATG6140.10978832667570244No Hit
GTCGTGACTGGGAAAACCCTGGATACTTGTCGTATAGACCGTCTGCGATG6110.10925190162679836No Hit
GTCGTGACTGGGAAAACCCTGGTCAATAGTCGTGATAGGAATGCGCGATG6070.10853666822825958No Hit
GTCGTGACTGGGAAAACCCTGGTTATAGGTCGTGATGAGACGGCTCGATG6010.10746381813045142No Hit
GTCGTGACTGGGAAAACCCTGGAAAAAGGTCGTGATTGCCGCGACCGATG6010.10746381813045142No Hit
GTCGTGACTGGGAAAACCCTGGTATTACGTCGTGATCGTGGGACTCGATG6000.10728500978081673No Hit
GTCGTGACTGGGAAAACCCTGGTTGACAGTCGTGATAGGTGTATACGATG5940.10621215968300857No Hit
GTCGTGACTGGGAAAACCCTGGGCCCTGGTCGTGATTCAAAGTATCGATG5900.10549692628446979No Hit
GTCGTGACTGGGAAAACCCTGGAGTTGGGTCGTGATATGCACTCGCGATG5880.10513930958520039No Hit
GTCGTGACTGGGAAAACCCTGGTGGGCCGTCGTGATGCTGCTTTACGATG5840.10442407618666161No Hit
GTCGTGACTGGGAAAACCCTGGAGAGTTGTCGTGATGACCACGGGCGATG5670.10138433424287181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACT350.0242.301243
CGTTACA201.6496779E-7242.301243
TCGTGAC534850.0241.207662
ACTGGGA532750.0240.131847
TGGGAAA521450.0239.556149
GTGACTG539800.0238.716724
TGACTGG540350.0238.341455
CGTGACT541450.0237.624833
GACTGGG543000.0237.114626
CTGGGAA552450.0232.795738
CGTGGCT9350.0222.865333
GTGACTT2500.0188.893524
TCGTGGC9800.0179.349822
TCGTTAC900.0175.08942
TGGGAAC14550.0174.684549
TCGAGAC600.0161.620972
CGAGACT650.0149.108463
GCGTGGC2950.0143.815292
GTCGTGG12750.0142.683431
TCGGGAC1800.0134.684162
Produced by FastQC (version 0.11.5)