FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924229
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT1828200.0241.622153
TGACTGG1838900.0241.48785
GTGACTG1838750.0241.41134
TCGTGAC1835350.0241.382642
GACTGGG1843200.0240.991646
ACTGGGA1833500.0240.689947
TGGGAAA1805700.0235.198369
CTGGGAA1919450.0230.820318
CGTGGCT35300.0215.859973
TCGTGGC31000.0206.408042
GTGGTGA18900.0185.441541
GCGTGGC9450.0171.841232
GGTGACT20550.0168.00773
GTGACTT9550.0162.330864
GTCGTGG41000.0160.319531
CGTGACA7100.0158.679493
TGCGAAA1800.0147.943629
TGACTTG10950.0144.845445
GTGGCTG54650.0143.1654
CTTGGAA12400.0137.63968
Produced by FastQC (version 0.11.5)