FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210922
Sequences flagged as poor quality0
Sequence length248
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGAGCGAAGTCGTGATCCCACTCCCCGATG44322.1012506993106457No Hit
GTCGTGACTGGGAAAACCCTGGACGGCCGTCGTGATTCGCGTCCTCGATG35411.6788196584519397No Hit
GTCGTGACTGGGAAAACCCTGGGGTTTGGTCGTGATACTAGATGACGAAT17520.8306388143484321No Hit
GTCGTGACTGGGAAAACCCTGGTCTTAGGTCGTGATGTTCCCGCCTGGTG11510.5456993580565328No Hit
GTCGTGACTGGGAAAACCCTGGTAACAGGTCGTGATTATAGGTAGCGAAT10920.5177269322308721No Hit
GTCGTGACTGGGAAAACCCTGGTAACAAGTCGTGATAAGCCAACTCGAAT10540.4997107935634974No Hit
GTCGTGACTGGGAAAACCCTGGAAGTATGTCGTGATATGTCCTCCCGATG7830.37122727833037805No Hit
GTCGTGACTGGGAAAACCCTGGTGAGCCGTCGTGATTGGAGTCCCCAATG3050.14460321825129668No Hit
GTCGTGACTGGGAAAACCCTGGGGAACCGTCGTGATGAAGGTTTTCGAAT2150.10193341614435668No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC195600.0241.709782
ACTGGGA197550.0240.668767
TGACTGG196950.0240.401175
GTGACTG197300.0240.334
GACTGGG199450.0238.970816
TGGGAAA194950.0238.912789
CGTGACT196950.0236.371343
CTGGGAA204950.0230.975378
CGTGGCT1050.0219.487673
ACGGGGA2000.0217.814737
GTGGTGA5200.0217.139371
TGACGGG2150.0208.444345
CGTGACA2800.0207.935683
GACGGGG2450.0207.590276
TGACCGG650.0204.977515
TGGTGAC5700.0195.915422
GTGACAG3400.0185.379064
TGACAGG3450.0182.56235
CTGCGAA205.0201448E-5181.512288
TGCGAAA205.0201448E-5181.512289
Produced by FastQC (version 0.11.5)