FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1897017
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGAAACCGTCGTGATCCCGGGTCGACTTG30670.16167488219662765No Hit
GTCGTGACTGGGAAAACCCTGGTCCATTGTCGTGACTTAACTGTGACTTG27980.14749472461237828No Hit
GTCGTGACTGGGAAAACCCTGGACGGCTGTCGTGATATCAAATACACTTG27530.14512257929159306No Hit
GTCGTGACTGGGAAAACCCTGGATATCAGTCGTGATGCGTAGGGTACTTG25460.1342107108159811No Hit
GTCGTGACTGGGAAAACCCTGGAAAGTGGTCGTGATCTGGACCCCACTTG24260.12788498996055386No Hit
GTCGTGACTGGGAAAACCCTGGAGAGCCGTCGTGATACCAAGAAGACTTG23460.12366784272360236No Hit
GTCGTGACTGGGAAAACCCTGGGTACCCGTCGTGATTCGGAAACCACTTG23360.12314069931898344No Hit
GTCGTGACTGGGAAAACCCTGGCCGGAGGTCGTGTCTGAAGACATACTTG22250.11728940752771325No Hit
GTCGTGACTGGGAAAACCCTGGCTCTGTGTCGTGATGCCATGATGACTTG21440.11301954595029987No Hit
GTCGTGACTGGGAAAACCCTGGCGAATGGTCGTGATGGGGGCACTACTTG21390.11275597424799039No Hit
GTCGTGACTGGGAAAACCCTGGAGTGCCGTCGTGATCAGTGAAGCACTTG20930.1103311145867433No Hit
GTCGTGACTGGGAAAACCCTGGGCCGTGGTCGTGATGGCAAGGGAACTTG20580.108486112670577No Hit
GTCGTGACTGGGAAAACCCTGGTACGATGTCGTGATTCGGATGCTACTTG19820.1044798227954731No Hit
GTCGTGACTGGGAAAACCCTGGCTCCTAGTCGTGGCTTACGTTCAACTTG19370.1021076774746879No Hit
GTCGTGACTGGGAAAACCCTGGCGCATCGTCGTGATAGCGGTGGGACTTG19340.10194953445330221No Hit
GTCGTGACTGGGAAAACCCTGGCCAGTCGTCGTGATAATGACGGAACTTG19060.1004735329203692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG1828750.0241.429344
TGACTGG1829200.0241.405565
CGTGACT1830750.0240.817693
GACTGGG1833250.0240.683066
TCGTGAC1837100.0240.568022
ACTGGGA1829850.0239.794727
CTGGGAA1872400.0234.753468
TGGGAAA1875600.0228.397499
TCGTGGC21500.0191.661512
ACTGGGG15050.0172.897677
CGTGGCT28250.0161.238593
ACTTGGA10200.0157.811517
TTGGAAA10650.0156.813089
GGCGTGG6250.0155.206861
GCGTGGC6150.0153.714192
TGGCTGG32400.0153.197625
CTTGGAA11550.0141.450558
CGTGACA6500.0137.917053
GTCGTGG30900.0136.951831
CGTTGCT1250.0135.680563
Produced by FastQC (version 0.11.5)