FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005493036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441636
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGGGACGGTCGTGATCGTAAGGGACAGTG133173.0153791810450232No Hit
GTCGTGACTGGGAAAACCCTGGGCCTTTGTCGTGATGAATAGCATCAGTG131712.98232028186108No Hit
GTCGTGACTGGGAAAACCCTGGGAGGCAGTCGTGATACTGAGATCCAGTG127592.8890307855337882No Hit
GTCGTGACTGGGAAAACCCTGGGAGTGAGTCGTGATACTGTGTTGCAGTG116082.628408915939824No Hit
GTCGTGACTGGGAAAACCCTGGATCGGAGTCGTGATCTAAAGAGACAGTG105472.3881658198154136No Hit
GTCGTGACTGGGAAAACCCTGGTGTGTCGTCGTGATGCCCGTTGACAGTG75311.7052504777690225No Hit
GTCGTGACTGGGAAAACCCTGGTCTGCTGTCGTGATCGTGAGAGCCAGTG70551.597469409196714No Hit
GTCGTGACTGGGAAAACCCTGGTGCGGCGTCGTGATTAGAGAGCCCAGTG59061.3373004012354064No Hit
GTCGTGACTGGGAAAACCCTGGGACTCCGTCGTGATTAGGCGTGCCAGTG58751.3302810459292267No Hit
GTCGTGACTGGGAAAACCCTGGCGGGTGGTCGTGATAAGTCTTTACAGTG57971.3126194422556132No Hit
GTCGTGACTGGGAAAACCCTGGGTAGAGGTCGTGATTGGTGTTTGCAGTG49281.1158510628662517No Hit
GTCGTGACTGGGAAAACCCTGGGCCCGTGTCGTGATGCAAAGTGGCAGTG46381.050186126131022No Hit
GTCGTGACTGGGAAAACCCTGGCCAATCGTCGTGATACAAGGAACCAGTG43140.9768225416406271No Hit
GTCGTGACTGGGAAAACCCTGGTAACTGGTCGTGAATGCAGCAGCCAGTG43050.9747846642936717No Hit
GTCGTGACTGGGAAAACCCTGGCGGTTCGTCGTGATTCGTAGCCGCAGTG42440.9609723844976406No Hit
GTCGTGACTGGGAAAACCCTGGTGGATCGTCGTGATATTATCGATCAGTG39490.8941752936807688No Hit
GTCGTGACTGGGAAAACCCTGGGCAACCGTCGTGATCCGGTGGGCCAGTG39350.8910052622521715No Hit
GTCGTTACTGGGAAAACCCTGGATTCACGTCGTGATGTTAAGTGGCAGTG36460.8255667563332699No Hit
GTCGTGACTGGGAAAACCCTGGCTGCACGTCGTGATATCGTGGGGCAGTG35380.8011122281698049No Hit
GTCGTGACTGGGAAAACCCTGGGTCACAGTCGTGATCCTCGTTCCCAGTG34850.7891113949044009No Hit
GTCGTGACTGGGAAAACCCTGGCTAAAAGTCGTGATAAGAAAATCCAGTG34540.782092039598221No Hit
GTCGTGACTGGGAAAACCCTGGCTCGCGGTCGTGATCCGGAGTTACAGTG33700.7630718510266373No Hit
GTCGTGACTGGGAAAACCCTGGTTAGTAGTCGTGATGCGATCACACAGTG31780.7195971342915886No Hit
GTCGTGACTGGGAAAACCCTGGAGTGCGGTCGTGATTGTCAGATCCAGTG31480.7128042098017371No Hit
GTCGTGACTGGGAAAACCCTGGGGATAAGTCGTGATTGATACTGCCAGTG31480.7128042098017371No Hit
GTCGTGACTGGGAAAACCCTGGCCTTGCGTCGTGATGATGAGTACCAGTG31220.7069170085771993No Hit
GTCGTGACTGGGAAAACCCTGGAATCTGGTCGTGATTCGCTGGAGCAGTG31160.705558423679229No Hit
GTCGTGACTGGGAAAACCCTGGGAAAATGTCGTGATATGGAACCCACAGT30330.6867646659239736No Hit
GTCGTGACTGGGAAAACCCTGGGGCTTGGTCGTGATATATAATAGCAGTG29480.6675180465360614No Hit
GTCGTGACTGGGAAAACCCTGGAGAATGGTCGTGATTCGGAGTTCCAGTG28970.655970074903314No Hit
GTCGTGACTGGGAAAACCCTGGTATTCAGTCGTGATATGAACGGCCAGTG28140.6371763171480586No Hit
GTCGTGACTGGGAAAACCCTGGAACGCAGTCGTGATGCCGAATGACAGTG26470.5993623708212192No Hit
GTCGTGACTGGGAAAACCCTGGTCTCTGGTCGTGATACAACGGGACAGTG25300.5728699653107989No Hit
GTCGTGACTGGGAAAACCCTGGGTATGAGTCGTGATACCAACTCCCAGTG25080.5678884873515746No Hit
GTCGTGACTGGGAAAACCCTGGCTCCTAGTCGTGATCTGTATCTTCAGTG24050.5445661132697516No Hit
GTCGTGACTGGGAAAACCCTGGAGTACGGTCGTGATACAGAAGGCCAGTG23900.541169651024826No Hit
GTCGTGACTGGGAAAACCCTGGCAAACTGTCGTGATCATTCTCCACAGTG22670.5133186606164353No Hit
GTCGTGACTGGGAAAACCCTGGGTAAGGGTCGTGATCTCTGCGGCCAGTG22320.505393582044942No Hit
GTCGTGACTGGGAAAACCCTGGGTAGAGGTCGTGATATGGTGATCCAGTG21890.4956570569428217No Hit
GTCGTGACTGGGAAAACCCTGGGTGCCTGTCGTGATCGTTACAAACAGTG21550.4879584091876568No Hit
GTCGTGACTGGGAAAACCCTGGCGGCATGTCGTGATAGTAAGGAGCAGTG21330.48297693122843244No Hit
GTCGTGACTGGGAAAACCCTGGGAGTCTGGTCGTGATCTAGTGGGCAGTG21040.4764104375549094No Hit
GTCGTGACTGGGAAAACCCTGGAATCAAGTCGTGATAAAAGTTTGCAGTG20680.46825892816708786No Hit
GTCGTGACTGGGAAAACCCTGGACTCCGGTCGTGATGACGAGGACCAGTG20630.4671267740854459No Hit
GTCGTGACTGGGAAAACCCTGGATGTTGGTCGTGATGCATAGTGGCAGTG20600.46644748163646077No Hit
GTCGTGACTGGGAAAACCCTGGAAAACAGTCGTGATCGGGATTTGCAGTG20580.4659946200038041No Hit
GTCGTGACTGGGAAAACCCTGGTTGGGGGTCGTGATGTGCAGGGACAGTG20540.4650888967384905No Hit
GTCGTGACTGGGAAAACCCTGGGACAACGTCGTGATTAGGAGTCTCAGTG20360.4610131420445797No Hit
GTCGTGACTGGGAAAACCCTGGCGGTTAGTCGTGTTCCCTCGGGCCAGTG20310.45988098796293775No Hit
GTCGTGACTGGGAAAACCCTGGTCTTTTGTCGTGATTAATAGTGCCAGTG20150.45625809490168373No Hit
GTCGTGACTGGGAAAACCCTGGCCCGTAGTCGTGATCCCGCAGAACAGTG19940.4515030477587878No Hit
GTCGTGACTGGGAAAACCCTGGCCGGCTGTCGTGATAACCCGTATCAGTG19740.4469744314322202No Hit
GTCGTGACTGGGAAAACCCTGGGTTCACGTCGTGATATTAACTCCCAGTG18590.4209348875544566No Hit
GTCGTGACTGGGAAAACCCTGGGTCTAAGTCGTGATCTTAGGTGACAGTG17800.40304685306451465No Hit
GTCGTGACTGGGAAAACCCTGGGCATCAGTCGCGATATTACGAACCAGTG17640.39942396000326064No Hit
GTCGTGACTGGGAAAACCCTGGATAGGTGTCGTGATTTGCACGGGCAGTG17240.39036672735012545No Hit
GTCGTGACTGGGAAAACCCTGGCAGGCCGTCGTGATTATAATTCTCAGTG17060.38629097265621465No Hit
GTCGTGACTGGGAAAACCCTGGGCCTCTGTCGTGATGTTAATGCTCAGTG16770.3797244789826916No Hit
GTCGTGACTGGGAAAACCCTGGCGACTGGTCGTGATAACAGTGGCCAGTG15950.36115715204376453No Hit
GTCGTGACTGGGAAAACCCTGGTTCTAAGTCTGATGCTACTAAGACAGTG15620.35368493510492804No Hit
GTCGTGACTGGGAAAACCCTGGCATGAGGTCGTGATCTGTCGAAACAGTG15580.3527792118396145No Hit
GTCGTGACTGGGAAAACCCTGGTTTTGCGTCGTGATTGTAAGGGGCAGTG15430.34938274959468885No Hit
GTCGTGACTGGGAAAACCCTGGGACCATGTCGTGATTGCCACGGCCAGTG15200.34417484081913613No Hit
GTCGTGACTGGGAAAACCCTGGCGCCCGGGCGCCGCAGAAGGGGTCAGTG15170.343495548370151No Hit
GTCGTGACTGGGAAAACCCTGGACCTGAGTCGTGATCCGGATGGGCAGTG14920.3378347779619415No Hit
GTCGTGACTGGGAAAACCCTGGTATACCGTCGTGATGGAAACCCTCAGTG14740.3337590232680307No Hit
GTCGTGACTGGGAAAACCCTGGTTCAGCGTCGTGATACCTAGAACCAGTG14670.33217400755373205No Hit
GTCGTGACTGGGAAAACCCTGGAATTAAGTCGTGATGACGATGGGCAGTG14110.3194938818393428No Hit
GTCGTGACTGGGAAAACCCTGGAACGCGGTCGTGATACATGAGTGCAGTG14040.31790886612504415No Hit
GTCGTGACTGGGAAAACCCTGGATGACCGTCGTGATTGTGGTGGGCAGTG13870.31405954224746174No Hit
GTCGTGACTGGGAAAACCCTGGATGCTGGTCGTGATAGTGATTTGCAGTG13620.30839877183925224No Hit
GTCGTGACTGGGAAAACCCTGGACCAACGTCGTGATGACACGTCACAGTG13600.30794591020659545No Hit
GTCGTGACTGGGAAAACCCTGGGGATGCGTCGTGTTTTAACTGGACAGTG13470.3050023095943265No Hit
GTCGTGACTGGGAAAACCCTGGGAGACTGTCGTGATTGAACCAACCAGTG13460.30477587877799817No Hit
GTCGTGACTGGGAAAACCCTGATTGGACGTCGTGTGCAGCACGGTCAGTG13270.30047369326775897No Hit
GTCGTGACTGGGAAAACCCTGGATGGTCGTCGTGATGTAAATTTGCAGTG13260.30024726245143063No Hit
GTCGTGACTGGGAAAACCCTGGCAACTCGTCGTGATTGTAGCACTCAGTG13100.29662436939017656No Hit
GTCGTGACTGGGAAAACCCTGGCAACCGGTCGTGATTGGCGGAGGCAGTG12900.29209575306360896No Hit
GTCGTGACTGGGAAAACCCTGGTTGGGGGTCGTGATGGCCGCAAGCAGTG12830.29051073734931027No Hit
GTCGTGACTGGGAAAACCCTGGAAAGGTGTCGTGATGCCGAGGGCCAGTG12730.2882464291860265No Hit
GTCGTGACTGGGAAAACCCTGGCGGTTCGTCGTGATAGGAGGGTTCAGTG12550.2841706744921157No Hit
GTCGTGACTGGGAAAACCCTGGGAAAAGGTCGTGATAATACATCCCAGTG12530.2837178128594589No Hit
GTCGTGACTGGGAAAACCCTGGACGCTCGTCGTGATGCTGGAGGGCAGTG11980.2712641179613981No Hit
GTCGTGACTGGGAAAACCCTGGCATCGTGTCGTGATAACAGAACCCAGTG11960.2708112563287413No Hit
GTCGTGACTGGGAAAACCCTGGTTCAGCGTCGTGATACCAAGAACCAGTG11960.2708112563287413No Hit
GTCGTGACTGGGAAAACCCTGGTGAGATGTCGTGATTACGAGCCTCAGTG11740.265829778369517No Hit
GTCGTGACTGGGAAAACCCTGGCCATCTGTCGTGATCACCAAGGGCAGTG11490.2601690079613075No Hit
GTCGTGACTGGGAAAACCCGGGGAGGCAGTCGTGATACTGAGATCCAGTG11350.2569989765327102No Hit
GTCGTGACTGGGAAAACCCTGGTTTATTGTCGTGATTAGGGACTCCAGTG11290.2556403916347399No Hit
GTCGTGACTGGGAAAACCCTGGCCGGGCGTCGTGATAAGGGGGGCCAGTG10910.24703602061426153No Hit
GTCGTGACTGGGAAAACCCTGGACACGCGTCGTGATTCGGATGGCCAGTG10840.24545100489996285No Hit
GTCGTGACTGGGAAAACCCTGGAAATTTGTCGTGATGGGTAGAGGCAGTG10720.24273383510402233No Hit
GTCGTGACTGGGAAAACCCTGGTCGCCTGTCGTGATTGAGTCGCCCAGTG10420.23594091061417094No Hit
GTCGTGACTGGGAAAACCCGGGGGGACGGTCGTGATCGTAAGGGACAGTG9920.224619369797752No Hit
GTCGTGACTGGGAAAACCCTGGAAACAAGTCGTGATTAATGGTTGCAGTG9870.2234872157161101No Hit
GTCGTGACTGGGAAAACCCTGGTCCAGAGTCGTGATAACAATGGCCAGTG9390.2126185365323479No Hit
GTCGTGACTGGGAAAACCCTGGCCTCTAGTCGTGATTTGTATGGCCAGTG9150.2071841969404668No Hit
GTCGTGACTGGGAAAACCCTGGAAAGGAGTCGTGATTATGATTGCCAGTG9110.20627847367515328No Hit
GTCGTGACTGGGAAAACCCTGGGGGTGCGTCGTGATACGAAGTTTCAGTG9080.20559918122616816No Hit
GTCGTGACTGGGAAAACCCTGGGTTGGTTGTCGTGATACTGTGTTCAGTG8930.20220271898124248No Hit
GTCGTGACTGGGAAAACCCTGGGAAAATGTCGTGATTATAGCTCTCAGTG8310.18816400836888297No Hit
GTCGTGACTGGGAAAACCCTGGACCTTTGTCGTGATGAACATTGCCAGTG8170.18499397694028566No Hit
GTCGTGACTGGGAAAACCCTGGATGATCGTCGTGATCCCGGACCCCAGTG8150.1845411153076289No Hit
GTCGTGACTGGGAAAACCCTGGTCCGACGTCGTGATTACGTAGAGCAGTG8010.18137108387903159No Hit
GTCGTGACTGGGAAAACCCTGGTCCAGCGTCGTGATGGCAAGTGGCAGTG7830.17729532918512078No Hit
GTCGTGACTGGGAAAACCCGGGGAGTGAGTCGTGATACTGTGTTGCAGTG7830.17729532918512078No Hit
GTCGTGACTGGGAAAACCCTGGGCATCAGTCGTGATATTACGAACCAGTG7790.17638960591980726No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGGGTCGTGATGGGTGGGTCCAGTG7640.17299314367488158No Hit
GTCGTGACTGGGAAAACCCGGGGCCTTTGTCGTGATGAATAGCATCAGTG7220.16348304938908967No Hit
GTCGTGACTGGGAAAACCCTGGCCCGGTGTCGTGATTTCATTCAGCAGTG6800.15397295510329773No Hit
GTCGTGACTGGGAAAACCCTGGTGCGATGTCGTGATAAGAAAGTGCAGTG6660.15080292367470044No Hit
GTCGTGACTGGGAAAACCCTGGAGACACGTCGTGATGCCGATGAACAGTG6640.15035006204204365No Hit
GTCGTGACTGGGAAAACCCTGGCATCGGGTCGTGATTTGGACGCCCAGTG6630.15012363122571531No Hit
GTCGTGACTGGGAAAACCCTGGCCGGGGGTCGTGATACCGCATCTCAGTG6620.14989720040938692No Hit
GTCGTGACTGGGAAAACCCTGGACCCGTGTCGTGATGTAGATAATCAGTG6370.14423643000117745No Hit
GTCGTGACTGGGAAAACCCTGGGATTCCGTCGTGATGAAGATCCGCAGTG5350.12114048673568277No Hit
GTCGTGACTGGGAAAACCCTGGTTCTAAGTGTGATGCTACTAAGACAGTG5120.11593257796013005No Hit
GTCGTGACTGGGAAAACCCTGGACCTAGGTCGTGATGCTAAGGAGCAGTG5070.11480042387848816No Hit
GTCGTGACTGGGAAAACCCTGGATGCGCGTCGTGATGGCCCGTTCCAGTG4950.11208325408254761No Hit
GTCGTGACTGGGAAAACCCTGGTGGCTGGTCGTGATAAAGTGGGCCAGTG4670.105743191225353No Hit
GTCGTGACTGGGAAAACCCGGGATCGGAGTCGTGATCTAAAGAGACAGTG4650.10529032959269624No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)