FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117092
Sequences flagged as poor quality0
Sequence length248
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTTAGTTGTCGTGATCTAGTCAGCCAGAT1327411.33638506473542No Hit
GTCGTGACTGGGAAAACCCTGGTCGGTGGTCGTGATGCCCGCCCTCAGAT100008.540293102859291No Hit
GTCGTGACTGGGAAAACCCTGGCCAATTGTCGTGATCCTTAGACCCAGAT85167.272913606394972No Hit
GTCGTGACTGGGAAAACCCTGGGCAGTAGTCGTGATGAAGCAATTCAGAT74966.401803709903324No Hit
GTCGTGACTGGGAAAACCCTGGGATGGTGTCGTGATACCTAGAAGCAGAT62275.31804051515048No Hit
GTCGTGACTGGGAAAACCCTGGCGCAAGGTCGTGATCCACCACCGCAGAT60305.149796741024152No Hit
GTCGTGACTGGGAAAACCCTGGTTACAGGTCGTGATCCCCTGGGGCAGAT53584.5758890445120075No Hit
GTCGTGACTGGGAAAACCCTGGCCGTGGTCCTGATCTCGCTAAAACAGAT51604.4067912410753936No Hit
GTCGTGACTGGGAAAACCCTGGCTGTAAGTCGTGATCATGTGTTCCAGAT50154.282956991083934No Hit
GTCGTGACTGGGAAAACCCTGGCCGTGGTCGTGATCTCGCTAAAACAGAT35733.0514467256516244No Hit
GTCGTGACTGGGAAAACCCTGGCCCGCCGTCGTGATGTACTCCAGCAGAT23462.0035527619307896No Hit
GTCGTGACTGGGAAAACCCTGGGTGAATGTCGTGATGTAAATGAACAGAT20421.743927851603867No Hit
GTCGTGACTGGGAAAACCCTGGTGAATTGTCGTGATACATACGAACAGAT14911.2733577016363202No Hit
GTCGTGACTGGGAAAACCCTGGGCCTGGGTCGTGATGCTAGGAAACAGAT10560.9018549516619411No Hit
GTCGTGACTGGGAAAACCCGGGTTAGTTGTCGTGATCTAGTCAGCCAGAT9940.8489051344242134No Hit
GTCGTGACTGGGAAAACCCGGGGCAGTAGTCGTGATGAAGCAATTCAGAT4090.349297987906945No Hit
GTCGTGACTGGGAAAACCCGGGGTGAATGTCGTGATGTAAATGAACAGAT3530.3014723465309329No Hit
GTCGTGACTGGGAAAACCCTGGCTGTAAGTCGTGATACCTACTTACAGAT2920.24937655860349126No Hit
GTCGTGACTGGGAAAACCCTGGAGCGACTGTCGTGAAGGAGGGCCCAGAT2850.24339835343148977No Hit
GTCGTGACTGGGAAAACCCGGGCCAATTGTCGTGATCCTTAGACCCAGAT2040.17422197929832953No Hit
GTCGTGACTGGGAAAACCCTGGACCCGTGTCGTGATTTCGAGGACCAGAT2030.1733679499880436No Hit
GTCGTGACTGGGAAAACCCTGGTTAGTAGTCGTGATCTAGTCAGCCAGAT1780.15201721723089534No Hit
GTGGTGACTGGGAAAACCCTGGTTAGTTGTCGTGATCTAGTCAGCCAGAT1700.14518498274860792No Hit
GTCGTGACAGGGAAAACCCTGGTTAGTTGTCGTGATCTAGTCAGCCAGAT1530.13066648447374712No Hit
GTCGTGACTGGGAAAACCCTGGATAGTTGTCGTGATCTAGTCAGCCAGAT1490.1272503672326034No Hit
GTCGTGACAGGGAAAACCCTGGTCGGTGGTCGTGATGCCCGCCCTCAGAT1340.11443992757831449No Hit
GTCGTGACTGGGAAAACCCGGGCCGTGGTCGTGATCTCGCTAAAACAGAT1250.10675366378574112No Hit
GTGGTGACTGGGAAAACCCGGGTTAGTTGTCGTGATCTAGTCAGCCAGAT1210.10333754654459741No Hit
GTGGTGACTGGGAAAACCCTGGCCCGCCGTCGTGATGTACTCCAGCAGAT1210.10333754654459741No Hit
GTGGTGACTGGGAAAACCCTGGCCGTGGTCGTGATCTCGCTAAAACAGAT1200.10248351723431148No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGGC301.8189894E-11242.395392
GCGTGAC700.0242.395392
TCGAGAC151.5819363E-5242.395392
AGGGCAT151.5819363E-5242.395392
GAGGGCA151.5819363E-5242.395391
GTCGAGA100.001522455242.395371
GACCACG201.6409649E-7242.395371
TCGTGGA100.001522455242.395372
TCGTGAC101450.0242.395372
AGCATGT100.001522455242.395372
GTCAATT100.001522455242.395371
TCCACAT100.001522455242.395372
ACCACGT100.001522455242.395372
CGTGAGT251.720764E-9242.188133
CGTGACT100350.0242.188133
CGTGCCT100.0015263606242.188113
CGTGACG650.0242.188113
CGTGACC151.5873486E-5242.188113
CCTAATG100.0015263606242.188113
GGGACTG700.0242.188114
Produced by FastQC (version 0.11.5)