FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044811
Sequences flagged as poor quality0
Sequence length248
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGATCAC40110.1317323144195157No Hit
GTCGTGACTGGGAAAACCCTGGACAACGGTCGTGATAAGTAGTTTTTCGC34190.11228939990035507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC2995300.0241.323842
ACTGGGA2987750.0241.072537
GTGACTG3010800.0239.892384
TGACTGG3012450.0239.777865
TGGGAAA3006750.0239.649779
GACTGGG3028950.0237.952886
CGTGACT3083400.0234.282033
CTGGGAA3111550.0231.512028
ACGTGAC5050.0163.118062
GACTGGA15650.0150.771586
TCGAGAC6750.0143.572312
GTCGTGA5841300.0123.784291
GACGTGA8100.0103.2401351
GTGACCG8250.099.764374
GTCGGAC3050.099.340111
TCGGACT3200.094.639952
CGAGACT11850.090.929823
CGGGGAA9900.086.774859
ACTGGAA28450.082.9321757
CTGCGAA4400.079.747318
Produced by FastQC (version 0.11.5)