FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106646
Sequences flagged as poor quality0
Sequence length248
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGATGCACGTCGTGATATCAAGAACCGATG43704.0976689233539No Hit
GTCGTGACTGGGAAAACCCTGGCTCTAAGTCGTGATAAGGAGACACGATG41183.8613731410460774No Hit
GTCGTGACTGGGAAAACCCTGGTGTACTGTCGTGATTCAGAACCCCGATG40113.761041201732836No Hit
GTCGTGACTGGGAAAACCCTGGACCCCAGTCGTGATGTCAGCGAGCGATG32003.000581362639011No Hit
GTCGTGACTGGGAAAACCCTGGTAGTAGGTCGTGATCTAACATCACGATG29342.7511580368696436No Hit
GTCGTGACTGGGAAAACCCTGGCCATGGGTCGTGATCGTGTGTCGCGATG20091.8838024867318042No Hit
GTCGTGACTGGGAAAACCCTGGCTATTCGTCGTGATCCTGACCCTCGATG18881.7703430039570165No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGGTCAAGCGTCGTGATACACGATG17881.6765748363745474No Hit
GTCGTGACTGGGAAAACCCTGGCTGGGCGTCGTGATACCAAGGCCCGACC17051.5987472572810981No Hit
GTCGTGACTGGGAAAACCCTGGCGTCATGTCGTGATATCCAAGAACGATG15901.4909138645612587No Hit
GTCGTGACTGGGAAAACCCTGGCCTATAGTCGTGATTGAGCGCCCCGACC15571.459970369259044No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGCCCTGTCATCAGGTCGTGCGATG13911.3043152110721452No Hit
GTCGTGACTGGGAAAACCCTGGCAAAGGGTCGTGATAAATGTGGACGATG13381.2546180822534365No Hit
GTCGTGACTGGGAAAACCCTGGATGCACGTCGTGATATCTAGAACCGATG13061.2246122686270466No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGCGTCGTGATATGCTGGTCCGATG11591.086773062280817No Hit
GTCGTGACTGGGAAAACCCTGGGCACAGGTCGTGATAAATAGTCCCAATG11081.0389512968137578No Hit
GTCGTGACTGGGAAAACCCTGTCAACTTCTTAGAGGGACAAGTGGCGACC10971.028636798379686No Hit
GTCGTGACTGGGAAAACCCTGGCCCCATGTCGTGATGCAAGCGTCCGATG10040.9414324025279898No Hit
GTCGTGACTGGGAAAACCCTGGTTGTATGTCGTGATATGTACCGACGACC9030.8467265532696959No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGGTCAATCGTCGTGATACACGATG7620.7145134369784146No Hit
GTCGTGACTGGGAAAACCCTGGCTATTCGTCGTGAACCTGACCCTCGATG5790.5429176903024961No Hit
GTCGTGACTGGGAAAACCCTGGCCGTCTGTCGTGATCGATGCGTCCGATG5620.5269771018134763No Hit
GTCGTGACTGGGAAAACCCTGGCCATGGCTCGTGATCGTGTGTCGCGATG4720.4425857509892541No Hit
GTCGTGACTGGGAAAACCCTGGCCTTCGTCCTGAGTAATATCAACGGAAG4650.4360219792584813No Hit
GTCGTGACTGGGAAAACCCTGGCCCTAAGTCGTGATCTGGGGTGACGATG4550.42664516250023443No Hit
GTCGTGACTGGGAAAACCCTGGATGCACGTCGTGATATCGAGAACCGATG4290.40226543892879246No Hit
GTCGTGACTGGGAAAACCCTGGCCTTCGTCGTGAGTAATATCAACGGAAG4270.4003900755771431No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGCGTCGTGATATGATGGTCCGATG3950.37038426195075297No Hit
GTCGTGACTGGGAAAACCCTGGAGCCGGGTCGTGATATCCAGAACCGATG3420.32068713313204433No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGGACAATCGTCGTGATACACGATG2590.242859554038595No Hit
GTCGTGACTGGGAAAACCCGGGCTCTAAGTCGTGATAAGGAGACACGATG2250.21097837706055547No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGCCCTGTCATCAGGTCGAGCGATG2250.21097837706055547No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGCCCTGGCATCAGGTCGTGCGATG2090.19597547024736042No Hit
GTCGTGACTGGGAAAACCCTGGCCATGGGTCGAGATCGTGTGTCGCGATG2030.1903493801924123No Hit
GTCGTGACTGGGAAAACCCTGGCCATGCGTCGTGATCGTGTGTCGCGATG1820.17065806500009378No Hit
GTCGTGACTGGGAAAACCCTGGCATCGGGTCGTGATGTGGCCAAACGATG1820.17065806500009378No Hit
GTCGTGACTGGGAAAACCCTGGCCATGGCTCGAGATCGTGTGTCGCGATG1760.16503197494514563No Hit
GTCGTGACTGGGAAAACCCTGTCAACTTCTTAGAGTGACAAGTGGCGACC1630.15284211315942464No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGCCCTGTCGTCAGGTCGTGCGATG1600.15002906813195055No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGGGCAATCGTCGTGATACACGATG1560.14627834142865181No Hit
GTCGTGACTGGGAAAACCCTGGCGTCATGTCGTGATATCCAAAAACGATG1490.13971456969787896No Hit
GTCGTGACTGGGAAAACCCTGGCCCGCTGTCGTGATGGACCCTCGCGATG1460.13690152467040487No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGCCCTGGCCTCAGGTCGTGCGATG1440.13502616131875553No Hit
GTCGTGACTGGGAAAACCCTGGCCGTCTGTCGTGATCGATGAGTCCGATG1410.13221311629128144No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGCCTCGTGATATGCTGGTCCGATG1340.1256493445605086No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGGTCAATCGACGTGATACACGATG1310.12283629953303453No Hit
GTCGTGACTGGGAAAACCCTGGTGAAGCGTCGTGATTTGAGAACCCGATG1260.11814789115391106No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGGACAAGCGTCGTGATACACGATG1170.10970875607148885No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGGTCAATCGTCGTGAAACACGATG1160.10877107439566415No Hit
GTCGTGACTGGGAAAACCCTGGCCATGGGTCGTGATCGTCTGTCGCGATG1150.10783339271983948No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGCCTCGTGATATGATGGTCCGATG1150.10783339271983948No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGCGTCGTGATAAGATGGTCCGATG1130.1059580293681901No Hit
GTCGTGACTGGGAAAACCCTGGCCATGGGTAGTGATCGTGTGTCGCGATG1090.10220730266489132No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGCGTCGTGATAAGCTGGTCCGATG1090.10220730266489132No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGGTCTAGCGTCGTGATACACGATG1090.10220730266489132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC102950.0242.106782
AGACTGG201.6537888E-7241.997215
TGACTGG102150.0241.878725
GTGACTG102450.0241.170474
CGTGACT102100.0241.162233
GACTGGG102800.0240.937856
TGGGAAA103500.0234.632059
ACTGGGA111600.0221.18027
CTGGGAA111200.0220.996548
TCGTGGC550.0220.204012
CGTGACA1300.0214.174883
CGAGACT255.017919E-7193.688583
GAGACTG255.0296876E-7193.597754
TGACGGG650.0186.151675
TGGTGAC1000.0181.66832
GGTGACT600.0181.583073
CGGGGAA600.0181.49798
GGGACTG401.3460522E-10181.49794
GACTGGA205.0148046E-5181.49796
CTGGGGA503.6379788E-12169.398048
Produced by FastQC (version 0.11.5)