FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005493042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581410
Sequences flagged as poor quality0
Sequence length248
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCCCATCGTCGTGATACTAAGTAACAGAT298065.126502812129134No Hit
GTCGTGACTGGGAAAACCCTGGAGCGACTGTCGTGAAGGAGGGCCCAGAT228923.937324779415559No Hit
GTCGTGACTGGGAAAACCCTGGGAGCGGGTCGTGATTAAACGGGTCAGAT227823.918405256187544No Hit
GTCGTGACTGGGAAAACCCTGGGCGGACGTCGTGATGATGCGCTCCAGAT227753.9172012865275794No Hit
GTCGTGACTGGGAAAACCCTGGATACGGGTCGTGATCACTAAGGACAGAT221653.8122839304449525No Hit
GTCGTGACTGGGAAAACCCTGGTGTGCCGTCGTGATATGGTCTTTCAGAT174853.0073442149257836No Hit
GTCGTGACTGGGAAAACCCTGGAAGGGTGTCGTGATACAATGAGCCAGAT168132.8917631275691855No Hit
GTCGTGACTGGGAAAACCCTGGCCCAGAGTCGTGATGTAAGCATGCAGAT148192.5488037701449926No Hit
GTCGTGACTGGGAAAACCCTGGGGTAATGTCGTGATAGCGGGACTCAGAT132832.2846184276156243No Hit
GTCGTGACTGGGAAAACCCTGGGAGTCAGTCGTGATAGGAACACCCAGAT117132.0145852324521423No Hit
GTCGTGACTGGGAAAACCCTGGAGTGAAGTCGTGATGAGATGTTCCAGAT94831.6310348979205727No Hit
GTCGTGACTGGGAAAACCCTGGACCATAGTCGTGATAATAACATCCAGAT85251.4662630501711358No Hit
GTCGTGACTGGGAAAACCCTGGCGCTCGGTCGTGATCCCACGGCGCAGAT85071.4631671281883696No Hit
GTCGTGACTGGGAAAACCCTGGCTCCCTGTCGTGATGTACGTTTCCAGAT82901.425844068729468No Hit
GTCGTGACTGGGAAAACCCTGGGTATTTGTCGTGATATAACAATTCAGAT82321.415868320118333No Hit
GTCGTGACTGGGAAAACCCTGGCAAGCCGTCGTGATGAACAAGGGCAGAT80711.388177017939148No Hit
GTCGTGACTGGGAAAACCCTGGCTGGATGTCGTGATAGGTCTTGGCAGAT80531.385081095956382No Hit
GTCGTGACTGGGAAAACCCTGGAGCTTGGTCGTGATCTAAAAGTGCAGAT77781.3377822878863452No Hit
GTCGTGACTGGGAAAACCCTGGCGGGGCGTCGTGATGAAAGAAGCCAGAT76281.3119829380299617No Hit
GTCGTGACTGGGAAAACCCTGGCGCTTTGTCGTGATCGAGAGACCCAGAT73361.2617602036428681No Hit
GTCGTGACTGGGAAAACCCTGGAGAAGCGTCGTGATATACATTGTCAGAT71151.2237491615211296No Hit
GTCGTGACTGGGAAAACCCTGGTTTCCGGTCGTGATCTACGTGTTCAGAT69671.1982938029961645No Hit
GTCGTGACTGGGAAAACCCTGGCGACGTGTCGTGATATCAAATACCAGAT66241.1392992896579006No Hit
GTCGTGACTGGGAAAACCCTGGGTACCGGTCGTGATCACCAGGGGCAGAT65941.134139419686624No Hit
GTCGTGACTGGGAAAACCCTGGACAGAGGTCGTGATACTGATATCCAGAT62981.0832287026366936No Hit
GTCGTGACTGGGAAAACCCTGGCCGTCTGTCGTGATTGAAAGGGGCAGAT62501.0749729106826507No Hit
GTCGTGACTGGGAAAACCCTGGATGAGCTGTCGTGATGGATATTACAGAT61761.062245231420168No Hit
GTCGTGACTGGGAAAACCCTGGCGAAGGGTCGTGATAAAAACCCCCAGAT58531.0066906313960888No Hit
GTCGTGACTGGGAAAACCCTGGCCGCGGGTCGTTGATGCTAGTAACAGAT57120.9824392425310882No Hit
GTCGTGACTGGGAAAACCCTGGGACGGAGTCGTGATGTTTGACGCCAGAT56850.9777953595569391No Hit
GTCGTGACTGGGAAAACCCTGGAAAGTTGTCGTGATTAGGTCTCCCAGAT56820.9772793725598115No Hit
GTCGTGACTGGGAAAACCCTGGACGATCGTCGTGATCATTGATCCCAGAT51880.8923135136994548No Hit
GTCGTGACTGGGAAAACCCTGGAAGCGGGTCGTGATTAAACGGGTCAGAT50860.8747699557971139No Hit
GTCGTGACTGGGAAAACCCTGGAGGAACGTCGTGAGAGAAATTCCCAGAT49520.851722536592078No Hit
GTCGTGACTGGGAAAACCCTGGGGGTGGGTCGTGATCATAAGGTGCAGAT46070.7923840319223956No Hit
GTCGTGACTGGGAAAACCCTGGGCATGTGTCGTGATATCTAGTAGCAGAT44960.7732925130286717No Hit
GTCGTGACTGGGAAAACCCTGGCTTGATGTCGTGATCTCGCGCCTCAGAT42650.733561514249841No Hit
GTCGTGACTGGGAAAACCCTGGTCATGGGTCGTGATACTAAGTAACAGAT38950.669923117937428No Hit
GTCGTGACTGGGAAAACCCTGGATTCCCGTCGTGATTTGACCGCGCAGAT36590.6293321408300511No Hit
GTCGTGACTGGGAAAACCCTGGAGGTCAGTCGTGATCGCAAGGGGCAGAT35820.6160884745704408No Hit
GTCGTGACTGGGAAAACCCTGGGACTCTGTCGTGATAAGAAGTCCCAGAT33410.5746375191345178No Hit
GTCGTGACTGGGAAAACCCTGGATCTGAGTCGTGATTACGAGGGGCAGAT32210.5539980392494109No Hit
GTCGTGACTGGGAAAACCCTGGGCCAGGGTCGTGATCTACGGGGACAGAT31930.5491821606095526No Hit
GTCGTGACTGGGAAAACCCTGGTATAGAGTCGTGATGCGTTAGGGCAGAT31370.5395504033298362No Hit
GTCGTGACTGGGAAAACCCTGGATAAACGTCGTGATCCATACGATCAGAT29530.5079032008393388No Hit
GTCGTGACTGGGAAAACCCTGGTAGCGAGTCGTGATTAGGATGCGCAGAT29210.5023993395366436No Hit
GTCGTGACTGGGAAAACCCTGGATAGTGGTCGTGATGTGAACGATCAGAT28110.4834798163086291No Hit
GTCGTGACTGGGAAAACCCTGGTCACGAGTCGTGATTCGGACAGCCAGAT25650.4411688825441599No Hit
GTCGTGACTGGGAAAACCCTGGTAAGACGTCGTGATTCGGAGTAACAGAT25190.4332570819215356No Hit
GTCGTGACTGGGAAAACCCTGGAGCCGGGTCGTGATAACGAGTATCAGAT25020.4303331556044788No Hit
GTCGTGACTGGGAAAACCCTGGGCTTCCGTCGTGATACGGTCACCCAGAT24730.42534528129891125No Hit
GTCGTGACTGGGAAAACCCTGGGCCTACGTCGTGATTACGATGGGCAGAT24280.4176054763419962No Hit
GTCGTGACTGGGAAAACCCTGGAACGTCGTCGTGATCTCTGAACACAGAT24150.41536953268777627No Hit
GTCGTGACTGGGAAAACCCTGGATGGACGTCGTGATAATGATCTCCAGAT20220.3477752360640512No Hit
GTCGTGACTGGGAAAACCCTGGTAGTTTGTCGTGATTCTACGTGGCAGAT19160.32954369549887347No Hit
GTCGTGACTGGGAAAACCCTGGGAAGCGGTCGTGATGCGCGTGGTCAGAT15500.2665932818492974No Hit
GTCGTGACTGGGAAAACCCTGGCCCCCTGTCGTGATTCCCTGCCCCAGAT14800.2545535852496517No Hit
GTCGTGACTGGGAAAACCCGGGGGTAATGTCGTGATAGCGGGACTCAGAT14650.25197365026401336No Hit
GTCGTGACTGGGAAAACCCTGGTATAGAGTCGTGGTGCGTTAGGGCAGAT14380.24732976728986428No Hit
GTCGTGACTGGGAAAACCCTGGTTAAGAGACGTGATGGCTCTCAGCAGAT12750.21929447377926076No Hit
GTCGTGACTGGGAAAACCCTGGCCAAGCGTCGTGATGAGTCCGCTCAGAT12340.2122426514851826No Hit
GTCGTGACTGGGAAAACCCGGGGAGCGGGTCGTGATTAAACGGGTCAGAT11840.20364286819972138No Hit
GTCGTGACTGGGAAAACCCTGGAATACCGTCGTGATACGATGTTCCAGAT8040.1382845152302162No Hit
GTCGTGACTGGGAAAACCCGGGAAGGGTGTCGTGATACAATGAGCCAGAT7640.13140468860184723No Hit
GTCGTGACTGGGAAAACCCGGGAGGAACGTCGTGAGAGAAATTCCCAGAT7250.1246968576391875No Hit
GTCGTGACTGGGAAAACCCTGGCCCTCGGTCGTGATTCTAGGTTCCAGAT7050.12125694432500302No Hit
GTCGTGACTGGGAAAACCCGGGCTGGATGTCGTGATAGGTCTTGGCAGAT7030.12091295299358457No Hit
GTCGTGACTGGGAAAACCCTGGTCGCTTGGTCGTGATTGACACACCAGAT6110.10508935174833593No Hit
GTCGTGACTGGGAAAACCCGGGGAGTCAGTCGTGATAGGAACACCCAGAT5860.10078946010560533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)