FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005493043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545144
Sequences flagged as poor quality0
Sequence length248
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCGGGATGTCGTGATATGTATTCTCGATG5530010.144108712560351No Hit
GTCGTGACTGGGAAAACCCTGGCCCCCTGTCGTGATCATAAGGCTCGATG356256.5349705765816No Hit
GTCGTGACTGGGAAAACCCTGGCGAGAAGTCGTGATCGAAATGGCCGATG264244.847159649560483No Hit
GTCGTGACTGGGAAAACCCTGGTATCTGGTCGTGATCGCTTTACCCGATG242394.446348120863478No Hit
GTCGTGACTGGGAAAACCCTGGTCGCAAGTCGTGATATTTAACCGCGATG236684.341605153867602No Hit
GTCGTGACTGGGAAAACCCTGGCGCACAGTCGTGATAAAATGTCGCGATG178793.2796839000337523No Hit
GTCGTGACTGGGAAAACCCTGGACCGCCGTCGTGATGGCCGTGGGCGATG172793.169621237691326No Hit
GTCGTGACTGGGAAAACCCTGGAGTGAAGTCGTGATCCCATGCAGCGATG165053.027640403269595No Hit
GTCGTGACTGGGAAAACCCTGGGCGCAGGTCGTGATAAAACGGACCGATG153812.821456349148115No Hit
GTCGTGACTGGGAAAACCCTGGACTTAGGTCGTGATGCAGAGGATCGATG147182.699837107259733No Hit
GTCGTGACTGGGAAAACCCTGGTTTTGCGTCGTGATAAAAACCCGCGATG139922.5666612858253965No Hit
GTCGTGACTGGGAAAACCCTGGGGAGGAGTCGTGATGCGGAGTCCCGATG122502.247112689491217No Hit
GTCGTGACTGGGAAAACCCTGGCTAAGGGTCGTGATCAAGGACCGCGATG104821.9227947111221988No Hit
GTCGTGACTGGGAAAACCCTGGGGTGGAGTCGTGATCGAACACCGCGATG104751.921510646728204No Hit
GTCGTGACTGGGAAAACCCTGGTCCCGCGTCGTGATTCTGTTCCCCGATG104651.9196762690224967No Hit
GTCGTGACTGGGAAAACCCTGGAGAACAGTCGTGATAGAATCGGCCGATG74761.3713807727866398No Hit
GTCGTGACTGGGAAAACCCTGGGTCAGGGTCGTGATGTAAAGTCCCGATG68381.2543474751625259No Hit
GTCGTGACTGGGAAAACCCTGGAGGCTTGTCGTGATATGGCGACACGATG66021.2110561613078379No Hit
GTCGTGACTGGGAAAACCCTGGACACGAGTCGTGATGCTTATTACCGATG57461.0540334296993088No Hit
GTCGTGACTGGGAAAACCCTGGCCCTTGGTCGTGATACACGTACCCGATG53160.9751551883539029No Hit
GTCGTGACTGGGAAAACCCTGGGGGCAGGTCGTGATACTAACCCACGATG48240.8849038052331127No Hit
GTCGTGACTGGGAAAACCCTGGTCCTCAGTCGTGATCAATACTGGCGATG47970.8799509854277034No Hit
GTCGTGACTGGGAAAACCCTGGTAGTTAGTCGTGATTCCGATGGCCGATG46130.8461984356426925No Hit
GTCGTGACTGGGAAAACCCTGGTTAAAAGTCGTGATTCGGGTTAGCGATG43400.7961199242768884No Hit
GTCGTGACTGGGAAAACCCTGGCTTTGTGTCGTGATGTTCCCGTCCGATG43380.7957530487357469No Hit
GTCGTGACTGGGAAAACCCTGGTAACAGGTCGTGATATTTACACTCGATG39160.7183423095549065No Hit
GTCGTGACTGGGAAAACCCTGGAGGTCGGTCGTGATCGCAGGCTCCGACC36240.6647784805482588No Hit
GTCGTGACTGGGAAAACCCTGGGACACTGTCGTGATGCGGAGAACCGATG34810.638546879356647No Hit
GTCGTGACTGGGAAAACCCTGGTAGCTTGTCGTGATAAATTCAGACGACC33890.6216706044641416No Hit
GTCGTGACTGGGAAAACCCTGGAGACTTGTCGTGATATCCAGACCCGATG24010.44043408714027854No Hit
GTCGTGACTGGGAAAACCCTGGGGACGTGTCGTGGTCGGTCTGGCCGATG23500.4310787608411722No Hit
GTCGTGACTGGGAAAACCCGGGCGGGATGTCGTGATATGTATTCTCGATG21370.39200651570961065No Hit
GTCGTGACTGGGAAAACCCTGGGATATTGTCGTGATGGTACCTCCCGAAT21230.3894383869216207No Hit
GTCGTGACTGGGAAAACCCTGGGAGCCGTCGTGATGCCAAAGGCCCGATG19650.3604552191714483No Hit
GTCGTGACTGGGAAAACCCTGGGAGCCGTCCTGATGCCAAAGGCCCGATG19430.35641958821889264No Hit
GTCGTGACTGGGAAAACCCTGGCGTGTCGTCGTGATGGAATTGCGCGATG18600.3411942532615236No Hit
GTCGTGACTGGGAAAACCCGGGCGAGAAGTCGTGATCGAAATGGCCGATG16650.3054238880002348No Hit
GTCGTGACTGGGAAAACCCTGGAAAATAGTCGTGATAGTTAGTGGCGATG15680.2876304242548758No Hit
GTCGTGACTGGGAAAACCCTGGAGCGCTGTCGTGATTTATCCGCCCGATG15630.2867132354020222No Hit
GTCGTGACTGGGAAAACCCGGGAGTGAAGTCGTGATCCCATGCAGCGATG15610.2863463598608808No Hit
GTCGTGACTGGGAAAACCCTGGCACACGTCCTGATGTAAAGAACCCGATG13030.2390194150536372No Hit
GTCGTGACTGGGAAAACCCTGGTGGTTCGTCGTGATGGCCGTGGGCGATG11520.21132031169745977No Hit
GTCGTGACCGGGAAAACCCTGGAGTGAAGTCGTGATCCCATGCAGCGATG11480.21058656061517692No Hit
GTCGTGACTGGGAAAACCCGGGGGAGGAGTCGTGATGCGGAGTCCCGATG11450.21003624730346476No Hit
GTCGTGACTGGGAAAACCCGGGGGTGGAGTCGTGATCGAACACCGCGATG10260.1882071526055501No Hit
GTGGTGACTGGGAAAACCCTGGGTCAGGGTCGTGATGTAAAGTCCCGATG8900.1632596158079333No Hit
GTCGTGACTGGGAAAACCCTGGCCCCCTGTCGTGATCAAAAGGCTCGATG8440.1548214783616806No Hit
GTCGTGACTGGGAAAACCCGGGTATCTGGTCGTGATCGCTTTACCCGATG8170.14986865855627138No Hit
GTCGTGACTGGGAAAACCCTGGGTCCCTGTCGTGATGAATATTACCGATG7890.14473240098029144No Hit
GTCGTGACTGGGAAAACCCGGGTTTTGCGTCGTGATAAAAACCCGCGATG7590.1392292678631701No Hit
GTCGTGACTGGGAAAACCCTGGCACACGTCGTGATGTAAAGAACCCGATG7020.12877331494063954No Hit
GTCGTGACTGGGAAAACCCTGGAGAGGGGTCGTGATTATATGTGCCGATG6820.1251045595292253No Hit
GTCGTGACTGGGAAAACCCGGGTCGCAAGTCGTGATATTTAACCGCGATG6800.12473768398808388No Hit
GTCGTGACAGGGAAAACCCTGGCGGGATGTCGTGATATGTATTCTCGATG6700.12290330628237676No Hit
GTGGTGACTGGGAAAACCCTGGGGGCAGGTCGTGATACTAACCCACGATG6460.1185007997886797No Hit
GTCGTGACTGGGAAAACCCGGGCTAAGGGTCGTGATCAAGGACCGCGATG6270.11501548214783618No Hit
GTCGTGACTGGGAAAACCCTGGGATCATGTCGTGATCGAGAACTGCAATG5850.10731109578386627No Hit
GTCGTGACTGGGAAAACCCTGGCCCCCTGTCGTGATCATAAGGCACGATG5780.10602703138987131No Hit
GTCGTGACTGGGAAAACCCTGGCCCTACGTCGTGATGGCGTATTACGATG5780.10602703138987131No Hit
GTCGTGACTGGGAAAACCCTGGGAGCCGTCCTGATTCCAAAGGCCCGATG5710.10474296699587633No Hit
GTCGTGACTGGGAAAACCCTGGAAACCCGTCGTGATAGATCGCTACGATG5600.10272515151959849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)