FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797708
Sequences flagged as poor quality0
Sequence length248
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTAGG9190.11520506250407418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC782900.0241.119222
ACTGGGA781100.0240.985667
TGGGAAA786550.0239.423559
GTGACTG788000.0239.410844
TGACTGG790050.0238.7915
GACTGGG796400.0236.522376
CGTGACT806250.0234.215823
CTGGGAA816050.0230.605358
ACGTGAC2100.0184.641452
TCGAGAC1800.0161.561282
CGAGACT2300.0152.646623
GACTGGA3250.0137.766886
GACGTGA2950.0127.3641741
GTCGTGA1516300.0124.550551
TCGTGCT501.0833173E-7121.170962
CGGGGAA2400.0115.9547659
AATGGGA2050.0112.142887
GTCGGAC1450.0108.663311
CGGACTG1350.0107.6129463
TGCGAAA1650.095.330399
Produced by FastQC (version 0.11.5)