FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147267
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCGGGCTGTCGTGATCTTCAGCAGTCTTC67300.5866114862538537No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGACTTG16450.14338423401004302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC1126350.0241.146742
GTGACTG1125450.0241.138924
TGACTGG1125950.0241.119745
CGTGACT1128250.0240.583183
ACTGGGA1129550.0240.031137
GACTGGG1131250.0239.894276
CTGGGAA1153450.0235.057548
TGGGAAA1188950.0228.079839
GTCGTGA2190750.0123.9267961
GCGTGGC2450.0123.621242
TGACCGG3350.0104.7653665
CGTGGCT5550.0104.708813
ACGTGAC2700.0103.2008362
ACCGGGA2600.097.727287
GACCGGG3600.097.48156
GACGTGA3050.091.386031
ACTAGGA2200.087.996867
CGTGACC3450.087.731573
TCGTGGC4450.087.118252
GTGACTA2150.084.463454
Produced by FastQC (version 0.11.5)