FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882438
Sequences flagged as poor quality0
Sequence length248
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGAGCTGAGTCGTGATTGTATTAGATGACC46500.5269492020969179No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGACC13910.15763147099286295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC868650.0240.69222
ACTGGGA868950.0240.031167
TGACTGG871750.0239.662195
GTGACTG872750.0239.41384
TGGGAAA873500.0238.559889
GACTGGG875200.0238.551826
CGTGACT884750.0236.028563
CTGGGAA922950.0225.869728
TCGAGAC1700.0171.072172
TGCGAAA750.0145.197119
GTCGTGA1681950.0124.27631
ACCGGGA2200.0121.01137
CTGCGAA900.0121.004458
GACCGGG2750.0118.817836
CGAGACT2500.0116.2433243
TGACCGG2900.0112.678475
TCGCGAC551.9120853E-7110.160112
ACTGGAA3850.0106.8671347
CTCGGAA1007.2759576E-1296.8035668
GTGACCG3200.090.79454
Produced by FastQC (version 0.11.5)