FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120735
Sequences flagged as poor quality0
Sequence length248
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC1075800.0241.542912
GTGACTG1081650.0241.420234
TGACTGG1081950.0241.366555
GACTGGG1089600.0239.962366
ACTGGGA1082650.0239.670557
CGTGACT1082850.0239.269883
CTGGGAA1106700.0234.604818
TGGGAAA1062700.0232.249779
CGTGGCT13300.0201.254473
TCGTGGC12000.0199.923342
GGTGACT14700.0187.854843
ACTGGGG13950.0138.791527
GTGGTGA20650.0136.20711
TTGGAAA7200.0127.720179
GTCGTGA2101850.0123.621161
TCGGGAC6300.0121.165662
CGTTACT704.7293724E-11121.116953
CGTGACA4650.0119.814613
GTCGTGG21100.0117.214031
CGTGACG3750.0116.272273
Produced by FastQC (version 0.11.5)