FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005304
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGACTTG14310.14234500210881484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG986450.0241.352394
TCGTGAC987650.0241.140052
TGACTGG986950.0241.096565
CGTGACT988250.0240.947713
ACTGGGA987400.0240.656347
GACTGGG987850.0240.595156
CTGGGAA1017650.0233.276868
TGGGAAA1026000.0231.437339
ACGTGAC2350.0159.818442
ACCGGGA2100.0144.042887
GTCGTGA1923550.0123.819271
TGCGAAA2200.0115.49629
TCGTGGC2600.0107.173532
ACTGCGA2300.0105.213937
GACTGCG2350.0102.985586
ACTAGGA1200.0100.8300257
CGGGGAA2300.099.953239
GCGTGGC2200.099.124922
GTCGTCA1150.094.843481
GTGACTA1600.090.778624
Produced by FastQC (version 0.11.5)