FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160896
Sequences flagged as poor quality0
Sequence length248
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCACAATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCAGAT33710.29037915541099285Illumina Single End PCR Primer 1 (97% over 38bp)
GACCACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCAGAT32400.2790947681790617Illumina Single End PCR Primer 1 (97% over 38bp)
GACCACAATATCGGAACAGCGTCGTGTAGGGAAAGAGTGTAGATCCAGAT17390.14979808699487293Illumina Single End PCR Primer 1 (96% over 28bp)
GACCACAATATCGGAACACCGTCGTGTAGGCAAAGAGTGTAGATCCAGAT14180.12214703125861405No Hit
GACCACAATATCGGAACACCGTCGTGTAGGGAAAGAGTGTAGATCCAGAT14040.12094106621092673Illumina Single End PCR Primer 1 (96% over 28bp)
GACCACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCAGAT11730.10104264292408623Illumina Single End PCR Primer 1 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG662850.0241.204685
TCGTGAC668050.0241.049132
GTGACTG663700.0240.953024
ATATCGG193750.0240.935938
GACTGGG663750.0240.48436
CGTGACT667650.0240.133453
TATCGGA191850.0239.894739
AATATCG93950.0237.937967
CGCGAGA2350.0236.950944
ACTGGGA666950.0236.81597
CACGATA17700.0235.946884
ACGATAT21950.0235.463935
TGGGAAA659100.0235.355939
TAGATCG40650.0234.914637
CGATATC34900.0234.787816
AAGATCG87800.0233.651617
ATGATCG13600.0232.271137
GAGATCG63700.0231.80037
CACAATA34300.0230.808644
CTGGGAA687700.0230.796788
Produced by FastQC (version 0.11.5)