FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113564
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTTAAGAGTCGTGATTCTACGCCCCAGAT38730.3478021918812031No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCAGAT17700.15894910395810208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT1090750.0241.17883
TCGTGAC1093150.0240.868272
GTGACTG1092050.0240.815964
TGACTGG1093100.0240.552765
ACTGGGA1094550.0239.78147
GACTGGG1096200.0239.585486
CTGGGAA1119700.0234.363148
TGGGAAA1129700.0232.27799
TCGCGAC650.0186.348712
CGGGGAA2600.0144.264629
CGAGACT3450.0143.882953
ACCGGGA2750.0140.79557
TGCGAAA1300.0130.303539
ACGTGAC2300.0126.393052
TGACCGG4300.0123.821135
GTCGTGA2129650.0123.636721
CGTTACT602.2828317E-9121.072253
TCGAGAC3850.0119.553592
GACGTGA2500.0116.323431
CGCGACT1050.0115.30693
Produced by FastQC (version 0.11.5)