FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1229456
Sequences flagged as poor quality0
Sequence length248
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGACTCTGGTCGTGATTAGGCTGTCTTAGG34260.27865983003865125No Hit
GTCGTGACTGGGAAAACCCTGGGTGCCCGTCGTGATCGCAAGAGCTTAGG34020.27670774716622637No Hit
GTCGTGACTGGGAAAACCCTGGCCTGAAGTCGTGATGTTGGTGTGTTAGG33770.27467432750745047No Hit
GTCGTGACTGGGAAAACCCTGGGTACCTGTCGTGATCTGATGTGATTAGG33440.27199021355786623No Hit
GTCGTGACTGGGAAAACCCTGGATCCATGTCGTGATATAGAGGTATTAGG26530.21578649418930002No Hit
GTCGTGACTGGGAAAACCCTGGTCCAACGTCGTGATCAAGAGGGCTTAGG25290.20570073268177144No Hit
GTCGTGACTGGGAAAACCCTGGCTCGGCGTCGTGATATCTCGTTTTTAGG24650.20049517835530511No Hit
GTCGTGACTGGGAAAACCCTGGACATTAGTCGTGATATGAAGTTTTTAGG24080.195858981533296No Hit
GTCGTGACTGGGAAAACCCTGGACCAGTGTCGTGATAGTATCACTTTAGG23320.18967738577061724No Hit
GTCGTGACTGGGAAAACCCTGGACTGACGTCGTGATCAAAACAGCTTAGG22680.1844718314441509No Hit
GTCGTGACTGGGAAAACCCTGGAACTTGGTCGTGATGAAAAGCTTTTAGG22340.18170638070821565No Hit
GTCGTGACTGGGAAAACCCTGGACGTTAGTCGTGATAATCTATGGTTAGG22160.180242318553897No Hit
GTCGTGACTGGGAAAACCCTGGGGCATTGTCGTGATGATAAGAGTTTAGG22040.17926627711768456No Hit
GTCGTGACTGGGAAAACCCTGGCGTGGCGTCGTGATGTAAAGCCCTTAGG21740.17682617352715346No Hit
GTCGTGACTGGGAAAACCCTGGCCAAGTGTCGTGATACACCAACATTAGG21540.17519943780013275No Hit
GTCGTGACTGGGAAAACCCTGGACGCGAGTCGTGATAGGAAGTTGTTAGG21220.17259666063689957No Hit
GTCGTGACTGGGAAAACCCTGGAGTAATGTCGTGATCAGCTGGGTTTAGG21120.1717832927733892No Hit
GTCGTGACTGGGAAAACCCTGGCTTTACGTCGTGATTCAGCTTGCTTAGG20970.17056324097812367No Hit
GTCGTGACTGGGAAAACCCTGGTTTCACGTCGTGATACATTTCCCTTAGG20310.16519501307895526No Hit
GTCGTGACTGGGAAAACCCTGGGTGGGTGTCGTGATCCGCGGTAGTTAGG19840.16137218412045654No Hit
GTCGTGACTGGGAAAACCCTGGGTACATGGAAGATTAAGCGCCACTTAGG19750.1606401530432972No Hit
GTCGTGACTGGGAAAACCCTGGGTGCAGGTCGTGATGACCGGTAATTAGG19700.16023346911154202No Hit
GTCGTGACTGGGAAAACCCTGGTGGAATGTCGTGATAGATACACATTAGG19550.15901341731627647No Hit
GTCGTGACTGGGAAAACCCTGGACCGAAGTCGTGATCCGAAGTAGTTAGG19530.15885074374357439No Hit
GTCGTGACTGGGAAAACCCTGGTTCGCAGTCGTGATCTAACTTTTTTAGG19250.15657331372574537No Hit
GTCGTGACTGGGAAAACCCTGGTAGCACGTCGTGATCATTGGTATTTAGG19050.15494657799872463No Hit
GTCGTGACTGGGAAAACCCTGGTCGCCAGTCGTGATTACGAGGGGTTAGG18940.15405187334886322No Hit
GTCGTGACTGGGAAAACCCTGGCTCAGTGTCGTGATCAAGGATATTTAGG18630.1515304329719811No Hit
GTCGTGACTGGGAAAACCCTGGTGGTGAGTCGTGATATGGCGGATTTAGG18530.15071706510847074No Hit
GTCGTGACTGGGAAAACCCTGGCCAACCGTCGTGATCTATGACTTTTAGG17870.14534883720930233No Hit
GTCGTGACTGGGAAAACCCTGGCCAAAGGTCGTGATTAGGCGCCTTTAGG17850.14518616363660025No Hit
GTCGTGACTGGGAAAACCCTGGACAAGAGTCGTGATGCCAAGACGTTAGG17790.14469814291849403No Hit
GTCGTGACTGGGAAAACCCTGGCATTATGTCGTGATCTGAGCATGTTAGG17640.14347809112322849No Hit
GTCGTGACTGGGAAAACCCTGGTATCCTGTCGTGATTGTGAGGCTTTAGG17590.14307140719147332No Hit
GTCGTGACTGGGAAAACCCTGGTCCTGAGTCGTGATTGCCACGGGTTAGG17540.14266472325971813No Hit
GTCGTGACTGGGAAAACCCTGGAAGCAAGTCGTGATTACTATGACTTAGG17400.1415260082508036No Hit
GTCGTGACTGGGAAAACCCTGGAAACGTGTCGTGATGGCCAGAGATTAGG17320.1408753139599953No Hit
GTCGTGACTGGGAAAACCCTGGGCATCCGTCGTGATACGCCGTGATTAGG17320.1408753139599953No Hit
GTCGTGACTGGGAAAACCCTGGGATGTCGTCGTGATCGCAACGAGTTAGG17040.1385978839421663No Hit
GTCGTGACTGGGAAAACCCTGGTACCAGTCGTGATACGTGGTGAATTAGG16480.13404302390650824No Hit
GTCGTGACTGGGAAAACCCTGGCGGTAGGTCGTGATGATACCACGTTAGG16220.13192826746138128No Hit
GTCGTGACTGGGAAAACCCTGGGGAATGGTCGTGATAAAAACAGTTTAGG16040.13046420530706265No Hit
GTCGTGACTGGGAAAACCCTGGATTCCGGTCGTGATTTGTGGTACTTAGG15910.12940682708449916No Hit
GTCGTGACTGGGAAAACCCTGGCTGTGAGTCGTGATTCCTTCAGCTTAGG15890.12924415351179708No Hit
GTCGTGACTGGGAAAACCCTGGAACCCGGTCGTGATAGAGAGGTTTTAGG15780.1283494488619357No Hit
GTCGTGACTGGGAAAACCCTGGTTACTGGTCGTGATTGCGCTAGCTTAGG15720.12786142814382948No Hit
GTCGTGACTGGGAAAACCCTGGGGGCACGTCGTGATGCAGAGTACTTAGG15680.1275360809984253No Hit
GTCGTGACTGGGAAAACCCTGGACGTGGGTCGTGATTTAAGCAGATTAGG15590.12680404992126598No Hit
GTCGTGACTGGGAAAACCCTGGGTCGATGTCGTGATCACCATATCTTAGG15560.12656003956221287No Hit
GTCGTGACTGGGAAAACCCTGGTAGAGGGTCGTGATTTAAGGTGATTAGG15400.1252586509805963No Hit
GTCGTGACTGGGAAAACCCTGGCACCTAGTCGTGATCAGGAGTGTTTAGG15390.12517731419424527No Hit
GTCGTGACTGGGAAAACCCTGGCTGCTGGTCGTGATGTTTAGGAATTAGG15360.12493330383519215No Hit
GTCGTGACTGGGAAAACCCTGGGCCGGAGTCGTGATCCAGGTAAATTAGG15290.1243639463307349No Hit
GTCGTGACTGGGAAAACCCTGGTCTCCCGTCGTGATAGAATGACATTAGG15210.1237132520399266No Hit
GTCGTGACTGGGAAAACCCTGGCCAGTGGTCGTGATATGTAGTATTTAGG15190.12355057846722454No Hit
GTCGTGACTGGGAAAACCCTGGTGAGGCGTCGTGATATGAATTAGTTAGG15020.1221678530992569No Hit
GTCGTGACTGGGAAAACCCTGGTGTGATGTCGTGATACCAATTTATTAGG14950.12159849559479965No Hit
GTCGTGACTGGGAAAACCCTGGTTTACCGTCGTGATAGGAACGAATTAGG14910.1212731484493955No Hit
GTCGTGACTGGGAAAACCCTGGTGTTGAGTCGTGATACCCGTTCGTTAGG14900.12119181166304446No Hit
GTCGTGACTGGGAAAACCCTGGTTCCAGGTCGTGATGTATATATGTTAGG14680.11940240236332166No Hit
GTCGTGACTGGGAAAACCCTGGGACTCCGTCGTGATGCAAACCATTTAGG14660.11923972879061959No Hit
GTCGTGACTGGGAAAACCCTGGTAGGGCGTCGTGATCTGATGCACTTAGG14580.1185890344998113No Hit
GTCGTGACTGGGAAAACCCTGGCGATTCGTCGTGATATGTTCAGGTTAGG14530.11818235056805612No Hit
GTCGTGACTGGGAAAACCCTGGACAAGTGTCGTGATTGTAGTTGCTTAGG14500.11793834020900301No Hit
GTCGTGACTGGGAAAACCCTGGTAGTTCGTCGTGATGCGGTTGGGTTAGG14490.11785700342265197No Hit
GTCGTGACTGGGAAAACCCTGGTAAAGTGTCGTGATTATTAGAAATTAGG14400.11712497234549264No Hit
GTCGTGACTGGGAAAACCCTGGCCCTAAGTCGTGACGCCTCTCCATTAGG14400.11712497234549264No Hit
GTCGTGACTGGGAAAACCCTGGTGCCAGGTCGTGATTTAGCATATTTAGG14250.1159049205502271No Hit
GTCGTGACTGGGAAAACCCTGGGAAACTGTCGTGATTGCATACATTTAGG14060.1143595216095574No Hit
GTCGTGACTGGGAAAACCCTGGATAGTAGTCGTGATTGCGCGGACTTAGG14010.1139528376778022No Hit
GTCGTGACTGGGAAAACCCTGGTTAATGGTCGTGATGAAAATAGATTAGG13790.11216342837807941No Hit
GTCGTGACTGGGAAAACCCTGGCTTAGCGTCGTGATTCGGGATAATTAGG13740.11175674444632423No Hit
GTCGTGACTGGGAAAACCCTGGAGTGCGGTCGTGCTGAATTGAGTTTAGG13630.11086203979646284No Hit
GTCGTGACTGGGAAAACCCTGGCTAATGGTCGTGATTCCTGGAGATTAGG13590.11053669265105867No Hit
GTCGTGACTGGGAAAACCCTGGAAGCTCGTCGTGATACGTTGAGATTAGG13520.10996733514660142No Hit
GTCGTGACTGGGAAAACCCTGGTCAATGGTCGTGATCATGTTGTTTTAGG13510.1098859983602504No Hit
GTCGTGACTGGGAAAACCCTGGTACACTGTCGTGATAAAGCTCCTTTAGG13430.10923530406944211No Hit
GTCGTGACTGGGAAAACCCTGGTAGGCAGTCGTGATACCAATGGGTTAGG13400.10899129371038899No Hit
GTCGTGACTGGGAAAACCCTGGGTTCTCGTCGTGATGAATTCTTCTTAGG13270.10793391548782551No Hit
GTCGTGACTGGGAAAACCCTGGAAGATGGTCGTGATTACATTGCATTAGG13260.10785257870147447No Hit
GTCGTGACTGGGAAAACCCTGGTGAGAAGTCGTGATAATCATGGCTTAGG13260.10785257870147447No Hit
GTCGTGACTGGGAAAACCCTGGACCCTCGTCGTGATTAGAAGGGGTTAGG13160.10703921083796411No Hit
GTCGTGACTGGGAAAACCCTGGCCTATCGTCGTGATGTAGTGACTTTAGG13020.10590049582904959No Hit
GTCGTGACTGGGAAAACCCTGGTAAGCTGTCGTGATCGACTACACTTAGG12980.10557514868364545No Hit
GTCGTGACTGGGAAAACCCTGGTGCCAGGTCGTGATTGCAAACGATTAGG12900.10492445439283715No Hit
GTCGTGACTGGGAAAACCCTGGCGGATGGTCGTGATTTACTAGGATTAGG12870.10468044403378406No Hit
GTCGTGACTGGGAAAACCCTGGAGAGCAGTCGTGATAGAGGGTTCTTAGG12860.10459910724743302No Hit
GTCGTGACTGGGAAAACCCTGGAACGGAGTCGTTATGTCCAGTACTTAGG12820.10427376010202886No Hit
GTCGTGACTGGGAAAACCCTGGGAAGTAGTCGTGATCTTGCTAAATTAGG12700.10329771866581643No Hit
GTCGTGACTGGGAAAACCCTGGCGAGCCGTCGTGATGGCAAGGGCTTAGG12660.10297237152041229No Hit
GTCGTGACTGGGAAAACCCTGGCTCCACGTCGTGATGAACGTCTTTTAGG12640.10280969794771021No Hit
GTCGTGACTGGGAAAACCCTGGACGTAGGTCGTGATTTGTGCAAGTTAGG12530.10191499329784881No Hit
GTCGTGACTGGGAAAACCCTGGATAAACGTCGTGATTTGCACGATTTAGG12480.10150830936609362No Hit
GTCGTGACTGGGAAAACCCTGGTAGAAGGTCGTGATTGTTTCACTTTAGG12460.10134563579339154No Hit
GTCGTGACTGGGAAAACCCTGGTTATTCGTCGTGATGCCGACAAATTAGG12400.10085761507528533No Hit
GTCGTGACTGGGAAAACCCTGGGCATATGTCGTGATAAGAAGGAGTTAGG12380.10069494150258325No Hit
GTCGTGACTGGGAAAACCCTGGTTCACGGTCGTGATATGATGAGCTTAGG12360.10053226792988118No Hit
GTCGTGACTGGGAAAACCCTGGAACCGAGTCGTGATAAGTTGTACTTAGG12360.10053226792988118No Hit
GTCGTGACTGGGAAAACCCTGGACACGTGTCGTGATCGCACGGCTTTAGG12300.10004424721177496No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC1189300.0240.735582
ACTGGGA1176700.0240.195947
TGGGAAA1153050.0239.160119
CGTGACT1211600.0235.887743
TGACTGG1210650.0235.759485
GTGACTG1213400.0235.701424
GACTGGG1214200.0233.924766
CTGGGAA1212550.0233.634028
CGTGGCT15800.0202.451193
CTGCGAA1500.0145.278438
TGGTGAC9100.0135.854432
GTCGGAC455.7492798E-8134.752871
GACTGGA10250.0133.467166
TGACAGG4300.0132.343415
TCGTGGC21900.0129.505072
CGTGACA4500.0126.549043
GTCGTGA2271750.0126.047531
TGCGAAA1550.0124.96569
TCGCGAC405.1981606E-6121.203462
GCGTTGC602.2664608E-9121.203452
Produced by FastQC (version 0.11.5)