FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980650
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGATCA14710.15000254932952634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT960350.0241.614093
TGACTGG961550.0241.365165
GTGACTG961700.0241.2384
TCGTGAC963350.0241.009232
GACTGGG964850.0240.30196
ACTGGGA965700.0239.952267
CTGGGAA988350.0234.37988
TGGGAAA989950.0234.037679
CGAGACT2100.0184.480743
ACGTGAC1700.0178.146552
TCGAGAC2600.0149.094942
TCGTTAC850.0142.517232
CGTTACT700.0138.360553
ACCGGGA3250.0126.594997
CGGGGAA2700.0125.4917769
GTCGTGA1877300.0123.609951
CGTGACC3100.0121.0654753
GCGTGGC1600.0113.568412
CCGTGAC1050.0103.833982
GACGTGA3050.0103.308781