FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779019
Sequences flagged as poor quality0
Sequence length248
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCTCTTTGTCGTGATGTCGTGGAATGACC16980.21796644241026214No Hit
GTCGTGACTGGGAAAACCCTGGATATTGGTCGTGATCAGCAAAGCTGACC15930.2044879521552106No Hit
GTCGTGACTGGGAAAACCCTGGTTGTTTGTCGTGATTAGAGCATATGACC15310.1965292245760373No Hit
GTCGTGACTGGGAAAACCCTGGGTACGCGTCGTGATGGAGAATACTGACC14800.189982529309298No Hit
GTCGTGACTGGGAAAACCCTGGGGTGGGGTCGTGATGGAGTGAATTGACC14090.180868502565406No Hit
GTCGTGACTGGGAAAACCCTGGAACTTCGTCGTGATGTGGCTTGTTGACC13710.1759905727588159No Hit
GTCGTGACTGGGAAAACCCTGGTGACAAGTCGTGATTAAAACCCGTGACC13370.17162610924765634No Hit
GTCGTGACTGGGAAAACCCTGGGCATCAGTCGTGATAACAGGACTTGACC12860.16507941398091702No Hit
GTCGTGACTGGGAAAACCCTGGGCCACCGTCGTGATTCTCGGACATGACC12810.16443758111162884No Hit
GTCGTGACTGGGAAAACCCTGGAATCGTGTCGTGATTGCCAAGGGTGACC12760.16379574824234067No Hit
GTCGTGACTGGGAAAACCCTGGCTCCAAGTCGTGATTGTCGAAACTGACC12350.1585327187141777No Hit
GTCGTGACTGGGAAAACCCTGGCATGGGGTCGTGATGCCTTCGGATGACC12330.15827598556646244No Hit
GTCGTGACTGGGAAAACCCTGGGTGTTGGTCGTGATTATTCGAGATGACC12320.1581476189926048No Hit
GTCGTGACTGGGAAAACCCTGGCGTTCGGTCGTGATTGAATAGGATGACC12190.15647885353245555No Hit
GTCGTGACTGGGAAAACCCTGGGTCTACGTCGTGATGGATAGCTCTGACC12030.15442498835073343No Hit
GTCGTGACTGGGAAAACCCTGGACGGCCGTCGTGATGAAAATGTATGACC11870.1523711231690113No Hit
GTCGTGACTGGGAAAACCCTGGGACTTCGTCGTGATAAGTATGGCTGACC11760.1509590908565773No Hit
GTCGTGACTGGGAAAACCCTGGCCGCTCGTCGTGATTATAACAGGTGACC11650.14954705854414335No Hit
GTCGTGACTGGGAAAACCCTGGCACGCGGTCGTGATGAATCTCCTTGACC11620.14916195882257044No Hit
GTCGTGACTGGGAAAACCCTGGACCTTAGTCGTGATCGGTAGGTTTGACC11570.1485201259532823No Hit
GTCGTGACTGGGAAAACCCTGGATCGCAGTCGTGATAGCTAGTAATGACC11480.14736482678856358No Hit
GTCGTGACTGGGAAAACCCTGGTCTATAGTCGGATATGAAGGCTGTGACC11290.14492586188526851No Hit
GTCGTGACTGGGAAAACCCTGGCAGCTAGTCGTGATACCGTCCCGTGACC11250.14441239558983798No Hit
GTCGTGACTGGGAAAACCCTGGACATTAGTCGTGATCGGACGGTCTGACC11210.14389892929440745No Hit
GTCGTGACTGGGAAAACCCTGGTTTCGTGTCGTGATCGGGGCTCCTGACC10980.14094649809568188No Hit
GTCGTGACTGGGAAAACCCTGGGCAACGGTCGTGATGCGAGACCCTGACC10900.13991956550482082No Hit
GTCGTGACTGGGAAAACCCTGGCGAGGGGTCGTGATCATACCATTTGACC10890.13979119893096317No Hit
GTCGTGACTGGGAAAACCCTGGGGCACCGTCGTGATACTGATCCGTGACC10750.13799406689695629No Hit
GTCGTGACTGGGAAAACCCTGGCGGATCGTCGTGATTATGATGGTTGACC10730.13773733374924105No Hit
GTCGTGACTGGGAAAACCCTGGATTTCGGTCGTGATGGGACCCGTTGACC10630.1364536680106647No Hit
GTCGTGACTGGGAAAACCCTGGCCAATGGTCGTGATTATACAGAGTGACC10630.1364536680106647No Hit
GTCGTGACTGGGAAAACCCTGGCCAGGTGTCGTGATTCTAATGCCTGACC10610.13619693486294943No Hit
GTCGTGACTGGGAAAACCCTGGACATCGGTCGTGATCATACCCTTTGACC10560.13555510199366128No Hit
GTCGTGACTGGGAAAACCCTGGTTGCCAGTCGTGATTGGATGTACTGACC10520.13504163569823074No Hit
GTCGTGACTGGGAAAACCCTGGGGTGGAGTCGTGATAAATGTAGCTGACC10480.1345281694028002No Hit
GTCGTGACTGGGAAAACCCTGGGTTTTCGTCGTGATTAAGGTTACTGACC10190.1308055387609288No Hit
GTCGTGACTGGGAAAACCCTGGCCTGTAGTCGTGATCAATATAGCTGACC10190.1308055387609288No Hit
GTCGTGACTGGGAAAACCCTGGAGAGATGTCGTGATAGAGATCCGTGACC10150.13029207246549826No Hit
GTCGTGACTGGGAAAACCCTGGCCAGCAGTCGTGATTCAACGACCTGACC10140.13016370589164064No Hit
GTCGTGACTGGGAAAACCCTGGTGGAGGGTCGTGATTGCATGGACTGACC10050.12900840672692193No Hit
GTCGTGACTGGGAAAACCCTGGAATTGGGTCGTGATGCGTACACCTGACC9980.1281098407099185No Hit
GTCGTGACTGGGAAAACCCTGGATAGCGGTCGTGATCTAGAGACATGACC9960.12785310756220325No Hit
GTCGTGACTGGGAAAACCCTGGTTCCTCGTCGTGATGCCGGAGCCTGACC9900.12708290811905743No Hit
GTCGTGACTGGGAAAACCCTGGCGCGTTGTCGTGATAATACACCCTGACC9810.12592760895433872No Hit
GTCGTGACTGGGAAAACCCTGGTCCTCGGTCGTGATCCGTGGCGGTGACC9700.12451557664190475No Hit
GTCGTGACTGGGAAAACCCTGGGAGTAGGTCGTGATGGGAACCCCTGACC9670.12413047692033186No Hit
GTCGTGACTGGGAAAACCCTGGGGCCAAGTCGTGATTCCTGGCCCTGACC9520.12220497831246734No Hit
GTCGTGACTGGGAAAACCCTGGCGGTAAGTCGTGATCGATAGTATTGACC9450.1213064122954639No Hit
GTCGTGACTGGGAAAACCCTGGTACCTCGTCGTGATGAGAATGACTGACC9420.12092131257389102No Hit
GTCGTGACTGGGAAAACCCTGGTATGATGTCGTGATCTGATCACATGACC9420.12092131257389102No Hit
GTCGTGACTGGGAAAACCCTGGAGACAGGTCGTGATCTTATCACTTGACC9390.1205362128523181No Hit
GTCGTGACTGGGAAAACCCTGGACTATCGTCGTGATTCCCTGCCCTGACC9350.12002274655688756No Hit
GTCGTGACTGGGAAAACCCTGGCTCGATGTCGTGATTTACAAAGCTGACC9320.11963764683531466No Hit
GTCGTGACTGGGAAAACCCTGGGGCAGAGTCGTATCAACGGTAGGTGACC9310.11950928026145703No Hit
GTCGTGACTGGGAAAACCCTGGTCCAAGGTCGTGATATTACAGCCTGACC9220.11835398109673832No Hit
GTCGTGACTGGGAAAACCCTGGTCCGTAGTCGTGATCAAAACTATTGACC9180.11784051480130779No Hit
GTCGTGACTGGGAAAACCCTGGCTGAAGGTCGTGATCGAATGAAGTGACC9170.11771214822745017No Hit
GTCGTGACTGGGAAAACCCTGGCATAAAGTCGTGATTGCAGGAGCTGACC9030.1159150161934433No Hit
GTCGTGACTGGGAAAACCCTGGTATCACGTCGTGAAAAAATACCCTGACC9010.11565828304572802No Hit
GTCGTGACTGGGAAAACCCTGGGTACCTGTCGTGATAAGGAAGAATGACC8990.11540154989801277No Hit
GTCGTGACTGGGAAAACCCTGGCTCCCAGTCGTGATCCCGGGATCTGACC8950.11488808360258222No Hit
GTCGTGACTGGGAAAACCCTGGAAGGGCGTCGTGATCTTGGATACTGACC8930.11463135045486694No Hit
GTCGTGACTGGGAAAACCCTGGCCCTACGTCGTGATATCTATGATTGACC8920.11450298388100934No Hit
GTCGTGACTGGGAAAACCCTGGAAATGAGTCGTGATTTGTACACCTGACC8920.11450298388100934No Hit
GTCGTGACTGGGAAAACCCTGGCCGCGGTCGTGATCGTATGTCCATGACC8860.11373278443786353No Hit
GTCGTGACTGGGAAAACCCTGGATCGCAGTCGTGATTAGCAGACTTGACC8820.11321931814243298No Hit
GTCGTGACTGGGAAAACCCTGGCTCAGTGTCGTGATGCAAAGAGCTGACC8790.1128342184208601No Hit
GTCGTGACTGGGAAAACCCTGGAAAGGAGTCGTGATGCGCTATCGTGACC8760.11244911869928717No Hit
GTCGTGACTGGGAAAACCCTGGAGATCCGTCGTGATACTAAGGGTTGACC8700.11167891925614139No Hit
GTCGTGACTGGGAAAACCCTGGCTCGTAGTCGTGATGCCCTAGGGTGACC8650.11103708638685321No Hit
GTCGTGACTGGGAAAACCCTGGACGCTAGTCGTGATACAAATGCGTGACC8640.11090871981299558No Hit
GTCGTGACTGGGAAAACCCTGGATATTCGTCGTGATGAAAATTAGTGACC8630.11078035323913793No Hit
GTCGTGACTGGGAAAACCCTGGCGTCCAGTCGTGATATCGTAGGATGACC8620.11065198666528031No Hit
GTCGTGACTGGGAAAACCCTGGGAATCGGTCGTGATAACCCATCTTGACC8600.11039525351756505No Hit
GTCGTGACTGGGAAAACCCTGGTAACCAGTCGTGATGTCAGCACCTGACC8530.10949668750056162No Hit
GTCGTGACTGGGAAAACCCTGGTCGAGTGTCGTGACGGACAATTATGACC8500.10911158777898869No Hit
GTCGTGACTGGGAAAACCCTGGTACCAGGTCGTGATGCGATTCCCTGACC8480.10885485463127344No Hit
GTCGTGACTGGGAAAACCCTGGAGTAGTGTCGTGATGCTGATCGCTGACC8450.10846975490970054No Hit
GTCGTGACTGGGAAAACCCTGGGCAACAGTCGTGATATTCACTGGTGACC8450.10846975490970054No Hit
GTCGTGACTGGGAAAACCCTGGCTCGGCGTCGTGATCGAGGTAGATGACC8400.10782792204041237No Hit
GTCGTGACTGGGAAAACCCTGGAGTCTCGTCGTGATCCGTACACCTGACC8400.10782792204041237No Hit
GTCGTGACTGGGAAAACCCTGGTATCTTGTCGTGATAATGTCGGTTGACC8370.10744282231883948No Hit
GTCGTGACTGGGAAAACCCTGGATTGCCGTCGTGATATGGATGACTGACC8300.10654425630183602No Hit
GTCGTGACTGGGAAAACCCTGGTCTCGAGTCGTGATGAGATTAAATGACC8220.10551732371097496No Hit
GTCGTGACTGGGAAAACCCTGGTAATCGGTCGTGATAGTAAGACTTGACC8190.10513222398940206No Hit
GTCGTGACTGGGAAAACCCTGGCGTTCAGTCGTGATTACGATGAGTGACC8190.10513222398940206No Hit
GTCGTGACTGGGAAAACCCTGGCCTTTTGTCGTGATTTAACCCCTTGACC8180.10500385741554442No Hit
GTCGTGACTGGGAAAACCCTGGGTCGACGTCGTGATTTAATGATATGACC8170.10487549084168678No Hit
GTCGTGACTGGGAAAACCCTGGCCTAACGTCGTGATAGCCTTCCCTGACC8130.10436202454625625No Hit
GTCGTGACTGGGAAAACCCTGGATTTCCGTCGTGATCGATGCCCATGACC8130.10436202454625625No Hit
GTCGTGACTGGGAAAACCCTGGACTACCGTCGTGATTACAATCCTTGACC8120.10423365797239861No Hit
GTCGTGACTGGGAAAACCCTGGAAGCTAGTCGTGATTTAATGATATGACC8120.10423365797239861No Hit
GTCGTGACTGGGAAAACCCTGGACATATGTCGTGATATGGAAGAGTGACC8090.10384855825082571No Hit
GTCGTGACTGGGAAAACCCTGGCCCGTGGTCGTGATGATGACCCGTGACC8060.10346345852925282No Hit
GTCGTGACTGGGAAAACCCTGGTAAACCGTCGTGATGGAAAGTTGTGACC8030.10307835880767992No Hit
GTCGTGACTGGGAAAACCCTGGAGAAATGTCGTGATTAATGGTGATGACC8010.10282162565996465No Hit
GTCGTGACTGGGAAAACCCTGGCCACGTGTCGTGATGTCGAAACGTGACC7990.10256489251224939No Hit
GTCGTGACTGGGAAAACCCTGGCTCCCTGTCGTGATACCGGGAGCTGACC7960.10217979279067647No Hit
GTCGTGACTGGGAAAACCCTGGTAATTAGTCGTGATATCATGACTTGACC7930.10179469306910358No Hit
GTCGTGACTGGGAAAACCCTGGCAGCATGTCGTGATTCGAAGAGCTGACC7890.10128122677367304No Hit
GTCGTGACTGGGAAAACCCTGGAAGTCGGTCGTGATACAAAGGCCTGACC7860.10089612705210015No Hit
GTCGTGACTGGGAAAACCCTGGCCCCCAGTCGTGATGCGCGGGAGTGACC7850.10076776047824251No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG720000.0240.048665
GTGACTG722300.0239.583154
TGGGAAA694850.0239.565759
TCGTGAC721300.0239.441092
GACTGGG723050.0238.939836
CGTGGCT25050.0238.821723
CGTGACT725250.0237.897723
ACTGGGA720300.0237.600897
CTGGGAA746450.0232.195188
GTGACTT3650.0202.300224
TCGTGGC23250.0196.472
GCGTGGC8550.0195.564872
CGTGACA2300.0194.817753
TCGACAA1100.0187.255312
GGCGTGG9150.0185.448961
TCGCGAC401.3460522E-10181.747792
GCTGGGG21200.0165.532037
CGTTACA453.074092E-10161.470553
TGCGAAA750.0161.335829
GTCGTGG28850.0160.905321
Produced by FastQC (version 0.11.5)