FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250926
Sequences flagged as poor quality0
Sequence length248
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCACAATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCAGAT20610.1647579473126308Illumina Single End PCR Primer 1 (97% over 38bp)
GACCACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCAGAT19860.15876238882236038Illumina Single End PCR Primer 1 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA100.0015296852242.105624
TGACTGG932050.0241.232485
GTGACTG931900.0241.131364
TCGTGAC937550.0240.599822
CGTGACT937050.0239.872913
GACTGGG938300.0239.32276
ACTGGGA934250.0239.271967
CGCAATA3850.0238.961384
TATCGGA125350.0237.326149
CTGGGAA949000.0236.08878
TATATCG26800.0235.734827
AAGATCG51350.0234.043627
TGGGAAA939150.0232.752769
ACGATAT11200.0230.188895
CACGATA8950.0227.227624
CGATATC19850.0226.814326
ACAATAT28100.0225.70845
TAGATCG27350.0224.799017
GAGATCG40150.0223.066447
CACAATA22500.0221.123124
Produced by FastQC (version 0.11.5)