FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004545
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCTCAATGTCGTGATACCGAGTCCACTTG18190.18107700501221946No Hit
GTCGTGACTGGGAAAACCCTGGCTCCTAGTCGTGATTCAAGTTGAACTTG18170.18087790989950675No Hit
GTCGTGACTGGGAAAACCCTGGTACCTTGTCGTGATAGGTCAGCCACTTG16900.16823537024224897No Hit
GTCGTGACTGGGAAAACCCTGGCAGACGGTCGTGATACGGATGATACTTG16290.162162969304511No Hit
GTCGTGACTGGGAAAACCCTGGTCTAATGTCGTGATCAGTTTGTCACTTG15910.1583801621629693No Hit
GTCGTGACTGGGAAAACCCTGGTCAGGTGTCGTGATATCAAGTCGACTTG15240.15171047588709316No Hit
GTCGTGACTGGGAAAACCCTGGCCCAGGGTCGTGATCACCCAGAGACTTG15030.1496199772036096No Hit
GTCGTGACTGGGAAAACCCTGGCAGCCAGTCGTGATCGGTTGCCCACTTG14840.14772857363283876No Hit
GTCGTGACTGGGAAAACCCTGGGACCGCGTCGTGATGATAATGATACTTG14690.14623536028749334No Hit
GTCGTGACTGGGAAAACCCTGGTATTCGGTCGTGATCTTATCTACACTTG14400.1433484811531589No Hit
GTCGTGACTGGGAAAACCCTGGGGCTTAGTCGTGATCTGTATAGCACTTG14300.1423530055895953No Hit
GTCGTGACTGGGAAAACCCTGGGCTACCGTCGTGATAGAGGTTAAACTTG13870.1380724606662718No Hit
GTCGTGACTGGGAAAACCCTGGTCCAGCGTCGTGATGTGGAGTTGACTTG13780.13717653265906454No Hit
GTCGTGACTGGGAAAACCCTGGATGGGGGTCGTGATCGCAACGGTACTTG13470.13409055841201736No Hit
GTCGTGACTGGGAAAACCCTGGCCACTTGTCGTGATGGTTACAGCACTTG13400.13339372551752285No Hit
GTCGTGACTGGGAAAACCCTGGTGGCTGGTCGTGATGCTACGGATACTTG13110.1305068463831884No Hit
GTCGTGACTGGGAAAACCCTGGTCCCGGGTCGTGATTCCAATGGAACTTG12950.12891408548148664No Hit
GTCGTGACTGGGAAAACCCTGGCAGCTAGTCGTGATTGCAATGACACTTG12780.12722177702342852No Hit
GTCGTGACTGGGAAAACCCTGGCCGTAGGTCGTGATTCTGAGTGAACTTG12700.12642539657257765No Hit
GTCGTGACTGGGAAAACCCTGGCCCGCCGTCGTGATTGCAAGTTGACTTG12670.12612675390350855No Hit
GTCGTGACTGGGAAAACCCTGGCGGACGGTCGTGATCGATGTGCAACTTG12580.1252308258963013No Hit
GTCGTGACTGGGAAAACCCTGGCTACGTGTCGTGATCTGGCGGGCACTTG12390.12333942232553047No Hit
GTCGTGACTGGGAAAACCCTGGGCCAAGGTCGTGATCAACGTTCAACTTG12210.12154756631111599No Hit
GTCGTGACTGGGAAAACCCTGGTAAGGCGTCGTGATATTTATGATACTTG12080.12025344807848329No Hit
GTCGTGACTGGGAAAACCCTGGTCCACCGTCGTGATGCCACGGGCACTTG11660.11607245071151616No Hit
GTCGTGACTGGGAAAACCCTGGACCTCGGTCGTGATCGTAGTGAAACTTG11650.11597290315515979No Hit
GTCGTGACTGGGAAAACCCTGGGGGCGCGTCGTGATGATGATGAAACTTG11620.11567426048609071No Hit
GTCGTGACTGGGAAAACCCTGGCCGCATGTCGTGATGTTGTATTGACTTG11610.11557471292973436No Hit
GTCGTGACTGGGAAAACCCTGGCCTGTAGTCGTGATCCTGATTTCACTTG11610.11557471292973436No Hit
GTCGTGACTGGGAAAACCCTGGAAGTGAGTCGTGATGCCTAGTATACTTG11610.11557471292973436No Hit
GTCGTGACTGGGAAAACCCTGGACCCGCGTCGTGATCAAGTGCCCACTTG11230.11179190578819266No Hit
GTCGTGACTGGGAAAACCCTGGCAACTAGTCGTGATTTACCAATCACTTG11150.11099552533734179No Hit
GTCGTGACTGGGAAAACCCTGGTTCCGAGTCGTGATTTAGAAGATACTTG11100.11049778755555999No Hit
GTCGTGACTGGGAAAACCCTGGTTGCATGTCGTGATCATAAGGACACTTG11050.11000004977377818No Hit
GTCGTGACTGGGAAAACCCTGGTTCATCGTCGTGATCGGATCGACACTTG11000.10950231199199638No Hit
GTCGTGACTGGGAAAACCCTGGTAGAACGTCGTGATCCTAATACCACTTG10990.10940276443564001No Hit
GTCGTGACTGGGAAAACCCTGGGCCCAAGTCGTGATTGCTATTGAACTTG10890.1084072888720764No Hit
GTCGTGACTGGGAAAACCCTGGAGCGTAGTCGTGATACACCACGAACTTG10820.1077104559775819No Hit
GTCGTGACTGGGAAAACCCTGGGCCAGTCGTGATTAGCTTGCCCTACTTG10700.10651588530130557No Hit
GTCGTGACTGGGAAAACCCTGGTATTCTGTCGTGATAGTACTGACACTTG10680.10631679018859284No Hit
GTCGTGACTGGGAAAACCCTGGGCTGCCGTCGTGATAAGAAGTGGACTTG10670.10621724263223649No Hit
GTCGTGACTGGGAAAACCCTGGCGAGCCGTCGTGATGCAGAAGGCACTTG10640.10591859996316741No Hit
GTCGTGACTGGGAAAACCCTGGAAGGCAGTCGTGATGTGCGGTTGACTTG10590.1054208621813856No Hit
GTCGTGACTGGGAAAACCCTGGTTTAATGTCGTGATGCGTACAGCACTTG10540.1049231243996038No Hit
GTCGTGACTGGGAAAACCCTGGTCTAAAGTCGTGATGAGAACGCAACTTG10520.10472402928689108No Hit
GTCGTGACTGGGAAAACCCTGGCGTTTAGTCGGATGTTACAAAAAACTTG10500.10452493417417835No Hit
GTCGTGACTGGGAAAACCCTGGGGCTATGTCGTATCATCAAGCTCACTTG10490.10442538661782201No Hit
GTCGTGACTGGGAAAACCCTGGACCTTAGTCGTGATTGAATCCCTACTTG10220.10173760259620027No Hit
GTCGTGACTGGGAAAACCCTGGTGCCAAGTCGTGTCTCTAGGTCCACTTG10200.10153850748348754No Hit
GTCGTGACTGGGAAAACCCTGGCCTCGGGTCGTGATATCGGTTAAACTTG10140.1009412221453494No Hit
GTCGTGACTGGGAAAACCCTGGACCGGCGTCGTGATGAAAATGAAACTTG10070.10024438925085487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG959100.0241.239795
GTGACTG960100.0240.99814
TCGTGAC962950.0240.53832
GACTGGG961750.0240.424646
CGTGACT962400.0240.103243
ACTGGGA962350.0238.779117
CTGGGAA983300.0235.107448
TGGGAAA978650.0228.963919
TCGTGGC15900.0220.306842
CGTGGCT19800.0215.360783
CGTGACA3300.0165.191483
TCGACTC750.0161.609162
GTCGTGG23050.0153.668851
GCGGGGC7650.0129.92112
GTCGTGA1876500.0123.4239651
CTTGGAA6650.0121.9600758
GGCGTGG7450.0118.8613051
GCGTGGC7450.0118.7664642
GTGGTGA10250.0118.345041
GTGGCTG37800.0116.2926944
Produced by FastQC (version 0.11.5)