FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256011
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTCAGAGCGTCGTGATGTGACACCACTTG61190.48717726198257816No Hit
GTCGTGACTGGGAAAACCCTGGTACCCAGTCGTGATAACATGGCAACTTG52870.42093580390617596No Hit
GTCGTGACTGGGAAAACCCTGGAAGGTCGTCGTGATACTGAGCCCACTTG43970.3500765518773323No Hit
GTCGTGACTGGGAAAACCCTGGCTAAAGGTCGTGATAAGTGTATCACTTG29360.23375591455807315No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGACTTG17630.14036501272679935No Hit
GTCGTGACTGGGAAAACCCTGGGATTAGGTCGTGATACTAGCCTCACTTG16250.12937784780547304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG1233600.0241.348364
TGACTGG1234300.0241.173875
TCGTGAC1235900.0240.924442
CGTGACT1237100.0240.713473
ACTGGGA1236300.0240.177357
GACTGGG1238700.0240.034336
CTGGGAA1272900.0233.12898
TGGGAAA1291800.0229.549489
GTCGTGA2403900.0123.818161
CGTTGCT602.2846507E-9121.061323
ACGTGAC3650.0112.841362
ACCGGGA2700.0112.026887
CGTGGCT6000.0106.93753
TCGTGGC5500.0105.7208942
GCGTGGC1900.0102.011392
ACTGGGG7750.0101.474687
CGTGACC3750.093.620763
ACTGCGA1700.092.5210347
GACCGGG4250.088.2508246
GACGTGA4700.087.6740651
Produced by FastQC (version 0.11.5)