FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528687
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCTAAACGTCGTGATTAGTCGTGCCGATG6590.12464842146676577No Hit
GTCGTGACTGGGAAAACCCTGGACGAAAGTCGTGATTAATCACTACGATG6120.11575847335001618No Hit
GTCGTGACTGGGAAAACCCTGGCCGAATGTCGTGATGCAGAAGCCCGATG6030.11405614285957477No Hit
GTCGTGACTGGGAAAACCCTGGCCCCATGTCGTGATGCTTAGGAACGATG5710.10800341222689418No Hit
GTCGTGACTGGGAAAACCCTGGATACTCGTCGTGATCACCATTCCCGATG5430.10270727292329866No Hit
GTCGTGACTGGGAAAACCCTGGAACGTAGTCGTGATAAAGACCCGCGATG5430.10270727292329866No Hit
GTCGTGACTGGGAAAACCCTGGCGGATCGTCGTGATAAGGAATATCGATG5330.10081579460058598No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT502600.0240.783513
TCGTGAC504000.0240.543622
ACTGGGA500550.0239.730137
TGGGAAA492550.0238.904199
CGTGGCT9350.0238.43883
CTGGGAA512850.0235.135538
GTGACTG517100.0234.181064
TGACTGG517300.0233.88245
GACTGGG518150.0233.266376
TGGGAAC13000.0171.314039
TCGTGGC11150.0170.654952
GTGACTT2500.0164.672764
CGTTGCT150.005131884161.550933
CGTTACA301.243443E-6161.550933
CGAGACT453.0559022E-10161.550923
TGCGAAA600.0161.382789
CTGCGAA557.2759576E-12154.047218
GTCGTGG13500.0144.649031
GTCGTGA962000.0126.131051
TCGTTGC405.1966363E-6121.197632
Produced by FastQC (version 0.11.5)