FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936750
Sequences flagged as poor quality0
Sequence length248
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTAGG13580.14496930878035763No Hit
GTCGTGACTGGGAAAACCCTGGCCGCAAGTCGTGATCGGTATTTCTTAAG13190.14080597811582599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC920200.0240.948172
ACTGGGA917050.0240.55487
GTGACTG920600.0240.492484
TGACTGG920700.0240.323325
TGGGAAA920000.0239.467829
GACTGGG924750.0238.81286
CGTGACT944000.0234.761063
CTGGGAA965200.0228.303748
ACGTGAC1400.0216.30762
TCGAGAC2150.0185.923922
ACTGGAA5200.0144.265037
GACTGGA5550.0137.362016
GTCGTGA1786500.0124.141891
GACGTGA2600.0121.1711041
GACTAGG1200.0121.00946
ACCGGGA2200.0120.9964757
ACTAGGA1200.0120.9964757
CGAGACT3750.0119.4469453
TGCGAAA950.0114.6282359
ACTCGGA1200.0110.913447
Produced by FastQC (version 0.11.5)