FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470447
Sequences flagged as poor quality0
Sequence length248
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGCGAGGGTCGTGATATCGTCAACTGACC82540.5613259097403716No Hit
GTCGTGACTGGGAAAACCCTGGTGACAAGTCGTGATCTGAAAAGCTGCCA65590.44605483910674776No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGACC18330.124655971959547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA1443000.0241.148827
TGACTGG1446650.0241.108815
GTGACTG1448500.0240.81614
GACTGGG1449650.0240.192646
TCGTGAC1454200.0240.176452
TGGGAAA1450200.0239.910039
CTGGGAA1494250.0232.756588
CGTGACT1523450.0229.038023
GACTGGA5650.0160.622096
CTGCGAA1850.0137.344248
TGCGAAA1900.0133.729939
TCGAGAC3100.0128.957642
CGGGGAA2950.0127.145969
GTCGTGA2820650.0123.814941
ACCGGGA3150.0115.236067
CGAGACT3750.0109.794343
GCGTGGC2600.0102.50482
GTGACCG4450.097.922534
GACCGGG5300.095.888366
ACTGGGG4550.095.7345667
Produced by FastQC (version 0.11.5)